Method and kit for detecting polymorphism of mitochondrial ND1 gene mononucleotide, and application of kit

A single nucleotide polymorphism and mitochondrial technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problem of increasing PCR product contamination, cannot achieve closed-tube operation, and is difficult to separate Type and other problems, to achieve the effect of low detection cost, convenient and simple detection, and easy operation
CN101775435AInactive Publication Date: 2010-07-14卫生部北京医院
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
卫生部北京医院
Publication Date
2010-07-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting SNP of mitochondrial ND1 gene mt DNA 3970 locus, a kit for detecting the SNP of the locus and application of the kit. The method does not have special restriction on samples, namely, either body fluid or histiocyte can be used as a detection sample, so the detection is convenient and rapid; auele specific primers designed by the method do not have strict requirement on the length of basic group of a region to be amplified, and PCR products can be obtained and high-resolution melting curve can be analyzed by using the auele specific primers; and before PCR reaction, saturated fluorescent dye is added, a light scanner detects fluorescent signals by optics and draws a temperature melting curve, so that wild type, heterozygous mutation and homozygous mutation can be accurately differentiated according to the curve. The invention has the advantages of simple and convenient operation, low detection cost, accurate detection result, and high application and market values.
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Description

technical field

[0001] The invention relates to a method for detecting the single nucleotide polymorphism (SNP) of the mitochondrial ND1 gene, in particular to a method for detecting the SNP at the mtDNA 3970 site of the mitochondrial ND1 gene and a kit for detecting the SNP at the site and Application of the kit. Background technique

[0002] Health and longevity has always been a research hotspot in various countries. This is because the long-lived elderly do not suffer from aging-related degenerative diseases such as stroke, cardiovascular disease, type 2 diabetes, Parkinson's disease, senile dementia, cancer, etc. during the aging process. And healthy life. These aging-related degenerative diseases not only bring a heavy economic burden to patients and affect the quality of life, but also since my country entered an aging society in 1998, the proportion of the elderly population in the total population (10%) has been increasing year by year. In 2020, the population over...

Claims

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