Methods for producing fermentation products
A technology for fermentation products and organisms, which can be used in fermentation, biofuels, etc., to solve the problems of complex, toxic and expensive fermentation organisms
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Embodiment 1
[0366] The purpose of this experiment was to investigate the effect of introducing pretreated lignocellulose-containing material (in this case PCS filtrate) into a fermentation process based on starch-containing material (in this case a corn mash fermentation (SSF) process) .
[0367] PCS filtrate
[0368] At pH 5 (using 1M NH 4 OH-adjusted PCS) and 50°C, unwashed, acid-treated steam-exploded PCS (ie, pretreated corn stover) was hydrolyzed at 15% TS (total solids) for 48 hours in a batch reactor. Cellulase preparation A was added at a dose of 5 mg-EP / g-TS (about 15 mg-EP / g-CEL). After hydrolysis, the PCS hydrolyzate was filtered in a Buchner funnel under vacuum using glass fiber filter paper (Whatman: GF / D), and finally the filtrate was sterile filtered. The PCS filtrate (PCS-f) was analyzed using HPLC (see Table 11). Hydrolysis yield represents 90% conversion of cellulose (glucose only).
[0369] Table 1 - Composition of PCS Filtrate. All concentrations are expressed in...
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