Preparation method of sanguinarine liposome with acid sensitivity
A technology of sanguinarine liposomes and plastids, applied in the field of preparation of sanguinarine liposomes, can solve the problems of loss of anticancer drugs, lack of targeting and immunogenicity, etc., achieve simple preparation process, improve in vivo and in vitro inhibition The effect of tumor rate and high encapsulation rate
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Embodiment 1
[0054] Take by weighing 1.6g soybean lecithin (purity is greater than 99% phosphatidylcholine), 160mg cholesterol is dissolved in 50mL ethanol, ultrasonic, dissolve; / L). The mixed solution was rotary evaporated at 50° C. for 2 h, and the ethanol was completely evaporated to dryness, and homogenized under high pressure to reduce the particle size (5000 psi, 3 times), to obtain blank liposomes before drug loading. Then the blank liposomes were placed in a dialysis bag, the two ends were tied tightly, put into 500 mL of normal saline for 2 hours, and the normal saline was changed every hour. Finally, dissolve 90 mg of sanguinarine in 90 mL of distilled water, mix it with the above-mentioned liposomes after dialysis, incubate in a water bath at 30°C for 5 min, and after sterile filtration (the pore size of the membrane filter is 0.2 μm), divide the subsequent filtrate into vials That's it. The molar ratio of sanguinarine: soybean lecithin: cholesterol is 1:7.5:1.5.
[0055] Th...
Embodiment 2
[0060] It is basically the same as Example 1, but there are the following changes: when preparing blank liposomes in the early stage, the operation process is: take 1g of soybean phospholipid (purity greater than 99% phosphatidylcholine), dissolve 160mg of cholesterol in 50mL of ether, ultrasonically, Dissolving; under the condition of 300rpm magnetic stirring, slowly inject the above solution into 30mL of ammonium citrate solution (300mmol / L), and the mixed solution was rotatated at 50°C for 2h, and the ether was completely evaporated to dryness, and the water bath was sonicated to reduce the particle size to obtain Blank liposomes before drug loading. Then the blank liposomes were placed in a dialysis bag, the two ends were tied tightly, put into 500 mL of normal saline for 2 hours, and the normal saline was changed every hour. Afterwards, the sanguinarine aqueous solution prepared in Example 1 was mixed with the above solution, incubated in a water bath at 30°C for 5 minute...
Embodiment 3
[0063] Take by weighing 1.6g soybean lecithin (purity is greater than 99%), 160mg cholesterol is dissolved in the dichloromethane, this solution is placed in the eggplant shape bottle of grind mouth, removes organic solvent under reduced pressure on 30 ℃ constant temperature water baths, makes phospholipid, Cholesterol forms a uniform film on the bottom of the bottle, and then put it in a vacuum desiccator to vacuum overnight, and set aside. In addition, 30 mL of 300 mmol / L ammonium citrate solution was added to the above-mentioned eggplant-shaped bottle, and the membrane was washed by rotating at 30° C. until milky white liposome suspension was formed, homogenized under high pressure to reduce the particle size (5000 psi, 3 times), to obtain blank liposomes before drug loading. Then the blank liposomes were placed in a dialysis bag, the two ends were tied tightly, put into 500 mL of normal saline for 2 hours, and the normal saline was changed every hour. Mix the sanguinarine...
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