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Method for separating and purifying coptisine monomers

A technology for separation and purification of coptisine, which is applied in the field of separation and purification of coptisine monomer, can solve the problems of lack of high purity, inability to strengthen the determination of coptisine content, and no coptisine, and achieves good separation effect, easy product quality, and ease of use. Amplify the effect of production

Inactive Publication Date: 2010-07-21
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to solve the above-mentioned problems such as the absence of high-purity coptisine separation and preparation method in the above-mentioned prior art, the lack of high-purity coptisine monomer compound, and the inability to strengthen the quality control of coptis and its preparations through the content determination of coptisine , providing a method for separation and purification of coptisine monomer

Method used

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  • Method for separating and purifying coptisine monomers
  • Method for separating and purifying coptisine monomers
  • Method for separating and purifying coptisine monomers

Examples

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Effect test

Embodiment 1

[0047] The method for separating and purifying coptisine monomer of the present embodiment comprises the following main steps:

[0048] (1), extraction:

[0049] Take 1 kg of Coptis chinensis medicinal materials, pulverize it into coarse powder, add 10 L of 85% ethanol solution by volume, extract by reflux for 3 times, 2 hours each time, filter, collect and combine the filtrate (the residues are evaporated to dryness to recover ethanol).

[0050] (2), concentrated:

[0051] The filtrate collected and merged in step (1) was recovered with ethanol, concentrated to a relative density of 1.10 g / mL (measured at about 90° C.), left to stand for crystallization for 8 hours, filtered, and the precipitate was removed (the crystal in the precipitate was barberry Alkali, separately purified and processed to obtain berberine product), coptisine is mainly retained in the filtrate, and the obtained filtrate (about 1.5L) is subjected to the next extraction treatment.

[0052] (3), extracti...

Embodiment 2

[0068] The method for separating and purifying coptisine monomer of the present embodiment comprises the following main steps:

[0069] (1), extraction:

[0070] Take 10 kg of Coptis chinensis medicinal materials, pulverize it into coarse powder, add 80 L of 95% ethanol solution by volume, extract by reflux for 4 times, 3 hours each time, filter, collect and combine the filtrate (the drug residues are evaporated to dryness to recover ethanol).

[0071] (2), concentrated:

[0072] The filtrate collected and merged in step (1) was recovered with ethanol, concentrated to a relative density of 1.05 g / mL (measured at about 90° C.), left to stand for crystallization for 12 hours, filtered, and the precipitate was removed (the crystal in the precipitate was barberry Alkali, separately purified treatment to obtain berberine product), coptisine is mainly retained in the filtrate, and the obtained filtrate (about 30L) is subjected to the next extraction treatment.

[0073] (3), extrac...

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Abstract

The invention discloses a method for separating and purifying coptisine monomers, comprising the steps of extracting, concentrating, leaching, dissolving and filtering, performing preparative high performance liquid chromatography and separating, recycling products and the like. The method of the invention has simple and convenient operation, short production cycle, high separating efficiency, stable process and low cost, and can realize preparing great amount of the high-purity coptisine monomers by separation; and the obtained coptisine monomers have high purity, and can be used as comparison products in assaying.

Description

technical field [0001] The invention relates to the technical field of methods for separating compound monomers from plants, in particular to a method for separating and purifying coptisine monomers. Background technique [0002] Rhizoma coptidis is the dry rhizome of Coptis chinensis Franch., Coptis deltoidea C.Y.Cheng et Hsiao or Coptis teetaWall. "Yalian", "Clouds". Coptis chinensis is bitter and cold in nature, and it returns to the heart, spleen, stomach, liver, gallbladder, and large intestine meridians. For damp-heat fullness, vomiting and acid swallowing, diarrhea, jaundice, high fever, dizziness, hyperactivity of heart fire, restlessness, insomnia, red eyes, toothache, diabetes, carbuncle and furuncle; external treatment of eczema, wet sore, ear canal pus. [0003] Coptisine is the characteristic chemical component of Coptis chinensis, and it is also the most active protoberberine alkaloid in Coptis chinensis. Its English name is Coptisine, and its chemical name ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/22
Inventor 文焕松郭建华刘丁夏柯
Owner CHENGDU PUSH BIOLOGICAL TECH
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