Steroid saponin extract of Smilax riparia A.DC. and preparation method and application thereof

A technology of brassic steroids and extracts, which is applied in the field of preparing products for the prevention and/or treatment of rheumatoid arthritis by using brass steroidal saponins extracts. See the research report on the biological activity of oxtail steroid saponins in rheumatoid arthritis and other issues

Inactive Publication Date: 2010-07-28
JILIN ACAD OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no high-content oxtail steroid saponin extract, especially products containing one or more of the 4 new compounds; Active research reports

Method used

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  • Steroid saponin extract of Smilax riparia A.DC. and preparation method and application thereof
  • Steroid saponin extract of Smilax riparia A.DC. and preparation method and application thereof
  • Steroid saponin extract of Smilax riparia A.DC. and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Take 20Kg of coarse powder of oxtail and rhizome, add 8 times the amount of water to reflux and extract 3 times, 2 hours each time, filter, combine the extracts, and recover the ethanol under reduced pressure to obtain the extract. Take the extract and dissolve it in water, add it to the HPD100 macroporous adsorption resin column, and elute with water until the eluent is nearly colorless, discard the eluent, and elute with 10% ethanol until the eluent is nearly colorless, discard the wash Deliquoring, eluting with 90% ethanol until the eluate is nearly colorless, collect the eluate, add the 90% ethanol eluate to the D941 decolorizing resin column, eluting with 6 times the column volume of 90% ethanol, and collect the eluate The liquid was concentrated under reduced pressure and dried to obtain 215 g of the steroidal saponins extract of the saponins, in which the content of steroidal saponins was 52%.

Embodiment 2

[0062] Take 20 kg of coarse powder of oxtail and rhizome, add 8 times the amount of 30% ethanol and reflux for 3 times, 2 hours each time, filter, combine the extracts, and recover the ethanol under reduced pressure to obtain the extract. Take the extract and dissolve it in water, add it to the HPD300 macroporous adsorption resin column, eluted with water until the eluent is almost colorless, discard the eluent, eluted with 50% ethanol until the eluent is almost colorless, discard the wash Deliquoring, eluting with 90% ethanol until the eluate is nearly colorless, collect the eluate, add the 90% ethanol eluate to the D941 decolorizing resin column, eluting with 6 times the column volume of 90% ethanol, and collect the eluate The liquid was concentrated under reduced pressure and dried to obtain 72 g of steroidal saponins extract of Cyclops vulgare, in which the content of steroidal saponins was 95%.

Embodiment 3

[0064] Take 20Kg of coarse powder of oxtail and rhizome, add 8 times the amount of 50% ethanol to reflux and extract 3 times for 2 hours each time, filter, combine the extracts, and recover the ethanol under reduced pressure to obtain the extract. Take the extract and dissolve it in water, add it to the HPD400 macroporous adsorption resin column, and elute with water until the eluent is nearly colorless, discard the eluent, and elute with 30% ethanol until the eluent is nearly colorless, discard the wash Deliquoring, eluting with 70% ethanol until the eluate is nearly colorless, collect the eluate, add the 70% ethanol eluate to the D941 decolorizing resin column, eluting with 6 times the column volume of 90% ethanol, and collect the eluate The liquid was concentrated under reduced pressure and dried to obtain 136 g of steroidal saponins extract of Cyclops, in which the content of steroidal saponins was 88%.

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Abstract

The invention provides a steroid saponin extract of Smilax riparia A.DC. and a preparation method and application thereof. Roots and rootstocks of Smilax riparia A.DC. are extracted by 0 to 100 percent aqueous solution of ethanol, and steroid saponin of the Smilax riparia A.DC. is separated by a macroporous adsorption resin method, wherein the steroid saponin accounts for 50 to 95 weight percent of the extract, and the extract contains one or more of four new compounds. The steroid saponin extract of the Smilax riparia A.DC. prepared is proved to have the effect of resisting rheumatoid arthritis and can be used for preparing products for preventing and / or treating the rheumatoid arthritis through pharmacological activity study.

Description

Technical field [0001] The invention belongs to the field of medicine, and relates to the steroidal saponins extract of sauerkraut and a preparation method thereof, as well as the use of the steroidal saponins extract of sauerkraut in preparing products for preventing and / or treating rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis is a common autoimmune disease characterized by chronic inflammatory lesions of joint tissues. The main pathological changes are joint synovial cell infiltration, synovial pannus formation, and erosion of cartilage and bone tissue. The purpose of rheumatoid arthritis treatment is to prevent the progression of the disease, that is, to prevent joint inflammation and destruction, and prevent loss of function. Although there are many treatment measures, drug treatment is still the basis. Current treatment drugs include non-steroidal anti-inflammatory drugs, steroidal anti-inflammatory drugs, and disease-modifying anti-rheumatic dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00C07J17/00A61K31/7048A61K31/704A61P29/00A61P19/02
Inventor 董方言王威师海波马恒
Owner JILIN ACAD OF TRADITIONAL CHINESE MEDICINE
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