Application of belamcanda chinensis total isoflavone or isoflavone compounds in preparing medicaments and food for preventing and treating women's disease

A technology of isoflavones and compounds, applied in the field of application of Shegan total isoflavones or isoflavone compounds in the preparation of medicines or food for preventing and treating women's diseases

Inactive Publication Date: 2010-08-11
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] So far, there is no report that the total isoflavones of Shegan or the above 12 isoflavones have the function of preventing and treating hypoestrogenism or menopause syndrome in women

Method used

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  • Application of belamcanda chinensis total isoflavone or isoflavone compounds in preparing medicaments and food for preventing and treating women's disease
  • Application of belamcanda chinensis total isoflavone or isoflavone compounds in preparing medicaments and food for preventing and treating women's disease
  • Application of belamcanda chinensis total isoflavone or isoflavone compounds in preparing medicaments and food for preventing and treating women's disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Preparation of Shegan Total Isoflavones

[0021] Shoot dry medicinal material 10Kg, heat extract three times with 10 times the volume of 80% ethanol aqueous solution according to the routine, each extraction time is 100 minutes, filter, combine the filtrate, recover the ethanol under reduced pressure, add water to dilute to 10 times the volume, and add AB- 8 macroporous resin columns, first eluted with water for 5 column bed volumes, then eluted with 90% ethanol aqueous solution for 5 column bed volumes, collected the 90% ethanol eluate, recovered ethanol under reduced pressure, concentrated and dried, and shot to dryness Total isoflavones 198g, total isoflavones were heated and refluxed with 30 times the amount of 80% ethanol to dissolve, filtered, the filtrate was concentrated under reduced pressure to about one-eighth volume, the concentrated solution was left overnight to precipitate, filtered, and dried to obtain total isoflavones product. According to ...

Embodiment 2

[0022] Example 2. Preparation of 12 isoflavone compounds such as iridin, irisin, irisaxanthin, and iridinin (see Jin Li (Jin Li), Chen Hai-sheng (Chen Haisheng), et al.Chemicalconstituents from Belamcanda chinensis Journal of Asian Natural Products Research 2008, 10(1): 89-91)

[0023] 4Kg of dried rhizomes of traditional Chinese medicine were crushed and extracted three times under reflux with 80% ethanol aqueous solution, and the solvent was recovered under reduced pressure to obtain about 200g of liquid extract, which was dispersed in 800ml of distilled water, and then washed with petroleum ether, chloroform and ethyl acetate respectively. Extracted 3 times (500ml each time), and recovered the solvent respectively to obtain 22g of petroleum ether extract, 65g of chloroform extract, and 28g of ethyl acetate extract.

[0024] Get chloroform extract 65g, carry out gradient solvent system for silica gel column chromatography: CHCl 3 →CHCl 3 / CH 3 OH:H 2 O (20:1), fractions ...

Embodiment 3

[0026] Example 3. Test of compound on estrogen-like effect in natural aging rats

[0027] 1. Experimental materials

[0028] (1) Experimental animals

[0029] 100 female SD rats aged 12 to 15 months, all of which were clean animals, were provided by the Animal Experiment Center of the Naval Medical Research Institute, and the Animal Qualification Certificate: SCXK (Army) 2007-011. The pre-experiment animals were acclimatized indoors for one week, the temperature of the feeding environment was 18-22°C, the relative humidity was (50±20)%, and they had free access to drinking water.

[0030] (2) Test drugs

[0031] 1. Shegan total isoflavones and 4 isoflavones: prepared by Example 1

[0032] (1) Shegan total isoflavones (BC), total isoflavones content ≥ 90%.

[0033] (2) Irisin (BC-1)

[0034] (3) Irisin (BC-2)

[0035] (4) Irisxanthin (BC-3)

[0036] (5) Irisin (BC-7

[0037] 2. Positive control drugs:

[0038] (1) Soybean isoflavones, batch number: 090118, Handan Maila...

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Abstract

The invention relates to the technical field of medicaments, in particular to application of 12 isoflavone compound monomers comprising belamcanda chinensis total isoflavone or tecoridin, wild tectoridin, tectorigenin, iridin and the like in preparing medicaments and food for preventing and treating lower women's estrogen level and retarding womb and ovarian failure or climacteric syndrome. Animal experiment results show that the total isoflavone or 12 isoflavone compound monomers in the belamcanda chinensis have remarkable pharmacological effects of improving the estrogen level, promoting the growth of follicle, promoting epitheliosis of womb and vagina, retarding womb and ovarian failure, improving the expression of an epithelial cell estrogen receptor of the vagina and the like, so the total isoflavone or 12 isoflavone compound monomers can be used for preparing the medicaments or food for preventing and treating lower women's estrogen level and retarding womb and ovarian failure or climacteric syndrome. The application provides a new source for seeking a plant estrogen medicament.

Description

technical field [0001] The invention relates to the technical field of medicine, which is total isoflavones or 12 isoflavone compound monomers irisin, iridogenin, irisaxanthin, subirididinin, Irisin, demethyliridin, 5,6,7,3'-tetrahydroxy-4'-methoxyisoflavone, 5,7-dihydroxy-6,3',4',5 '-tetramethoxyisoflavone, 5,7,3'-trihydroxy-8,4',5'-trimethoxyisoflavone, 5,7,4'-trihydroxy-6,3',5' -Trimethoxyisoflavone, irisin-4'-glucoside, 5,3',5'-trimethoxy-6,7-methylenedioxyisoflavone-4'-O-β-D- Glucose is used for preparing medicine or food for preventing and treating low estrogen level in women, slowing down uterine and ovarian recession or preventing and treating menopausal syndrome. Background technique [0002] Shegan is the dry rhizome of Belamcanda chinensis (L.) DC. The reported isoflavones contained in Shegan are mainly irisin, irisin, irisaxanthin, irisaxanthin, irisin, demethylirisanthin, 5, 6, 7, 3 '-tetrahydroxy-4'-methoxyisoflavone, 5,7-dihydroxy-6,3',4',5'-tetramethoxyis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K31/352A23L1/30A61P5/30A61P15/00A61P15/12A23L33/105
Inventor 陈海生金丽刘建国金永生赵卫权
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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