Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Modified culture method for promoting cloned mouse embryonic development

A technology of cloning embryos and culture methods, applied in tissue culture, biochemical equipment and methods, cells modified by introducing foreign genetic material, etc., can solve problems such as the impact of cloned embryos, and achieve the effect of improving the development rate

Inactive Publication Date: 2010-08-11
赵巍
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term effects of these drugs on cloned embryos, especially whether they can be safely used in therapeutic cloning, remains to be studied.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Modified culture method for promoting cloned mouse embryonic development
  • Modified culture method for promoting cloned mouse embryonic development
  • Modified culture method for promoting cloned mouse embryonic development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] Materials and Methods

[0014] 1 reagent

[0015] Unless otherwise specified, all reagents used in this study were products of Sigma (St.Louis, MO).

[0016] 2 test animals

[0017] B6D2F1 mice (C57BL / 6×DBA / 2, 8-12 weeks) were used to provide oocytes and granulosa cells, and C57BL / 6 (7-8 weeks) and ICR female mice (8-12 weeks) were used to provide fertilized eggs. 8-12 weeks ICR female mice also served as pseudopregnant recipients. The 8-12 week old ICR male mice are used as normal breeding male mice, and secondly, ligated and used as pseudopregnant male mice. DBA male mice, C57BL / 6 and ICR female mice and ICR male mice were purchased from Beijing Weitong Lihua Co., Ltd. B6D2F1 mice were bred in this laboratory, and all mice were kept in a clean environment.

[0018] 3 medium

[0019] Table 1 Components of each culture medium

[0020]

[0021]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a modified culture method for promoting cloned mouse embryonic development. The method is a continuous culture method-D culture method. The invention also relates to two culture systems capable of increasing the developmental rate of cloned mouse embryo, a method of using the culture systems for obtaining cloning animal and a method of using the culture systems for obtaining embryonic stem cells. By using the culture method of the invention, the developmental rate of cloned mouse embryo and the establishment efficiency of nuclear transfer of embryonic stem cells are increased, thus paving a new way for using clone technology in the production and facilitating the further development of animal cloning technology.

Description

technical field [0001] The present invention relates to a method for improving the developmental rate of mouse cloned embryos. The method is a continuous culture method-D culture method. The present invention also relates to two culture systems for improving the developmental rate of mouse cloned embryos. Using the culture system A method for obtaining somatic cell cloned animals, and a method for obtaining embryonic stem cells using the culture system. Background technique [0002] In recent years, the technique of somatic cell nuclear transfer (SCNT) has achieved success in many species, but the efficiency of SCNT is still relatively low. Studies suggest that the inefficiency of somatic cell nuclear transfer (SCNT) is largely caused by incomplete donor nuclear reprogramming (Jouneau et al., 2003; Latham 2004). In order to improve the efficiency of reprogramming, many methods have been tried, including the activation timing of reconstituted embryos and the influence of DMS...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/16C12N5/18A01K67/027
Inventor 周琪代相鹏郝捷王柳
Owner 赵巍
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com