Modified culture method for promoting cloned mouse embryonic development

A technology of cloning embryos and culture methods, applied in tissue culture, biochemical equipment and methods, cells modified by introducing foreign genetic material, etc., can solve problems such as the impact of cloned embryos, and achieve the effect of improving the development rate

Inactive Publication Date: 2010-08-11
赵巍
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, the long-term effects of these drugs on cloned embryos, especially whether they can be safely used in therapeutic cloning, remains to be studied.

Method used

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  • Modified culture method for promoting cloned mouse embryonic development
  • Modified culture method for promoting cloned mouse embryonic development
  • Modified culture method for promoting cloned mouse embryonic development

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Experimental program
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Embodiment Construction

[0013] Materials and Methods

[0014] 1 reagent

[0015] Unless otherwise specified, all reagents used in this study were products of Sigma (St.Louis, MO).

[0016] 2 test animals

[0017] B6D2F1 mice (C57BL / 6×DBA / 2, 8-12 weeks) were used to provide oocytes and granulosa cells, and C57BL / 6 (7-8 weeks) and ICR female mice (8-12 weeks) were used to provide fertilized eggs. 8-12 weeks ICR female mice also served as pseudopregnant recipients. The 8-12 week old ICR male mice are used as normal breeding male mice, and secondly, ligated and used as pseudopregnant male mice. DBA male mice, C57BL / 6 and ICR female mice and ICR male mice were purchased from Beijing Weitong Lihua Co., Ltd. B6D2F1 mice were bred in this laboratory, and all mice were kept in a clean environment.

[0018] 3 medium

[0019] Table 1 Components of each culture medium

[0020]

[0021]

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Abstract

The invention relates to a modified culture method for promoting cloned mouse embryonic development. The method is a continuous culture method-D culture method. The invention also relates to two culture systems capable of increasing the developmental rate of cloned mouse embryo, a method of using the culture systems for obtaining cloning animal and a method of using the culture systems for obtaining embryonic stem cells. By using the culture method of the invention, the developmental rate of cloned mouse embryo and the establishment efficiency of nuclear transfer of embryonic stem cells are increased, thus paving a new way for using clone technology in the production and facilitating the further development of animal cloning technology.

Description

technical field [0001] The present invention relates to a method for improving the developmental rate of mouse cloned embryos. The method is a continuous culture method-D culture method. The present invention also relates to two culture systems for improving the developmental rate of mouse cloned embryos. Using the culture system A method for obtaining somatic cell cloned animals, and a method for obtaining embryonic stem cells using the culture system. Background technique [0002] In recent years, the technique of somatic cell nuclear transfer (SCNT) has achieved success in many species, but the efficiency of SCNT is still relatively low. Studies suggest that the inefficiency of somatic cell nuclear transfer (SCNT) is largely caused by incomplete donor nuclear reprogramming (Jouneau et al., 2003; Latham 2004). In order to improve the efficiency of reprogramming, many methods have been tried, including the activation timing of reconstituted embryos and the influence of DMS...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/16C12N5/18A01K67/027
Inventor 周琪代相鹏郝捷王柳
Owner 赵巍
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