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Method for separating proteins by capillary electrophoresis and buffer compositions for capillary electrophoresis

A technology of capillary electrophoresis and protein, applied in the direction of material analysis by electromagnetic means, peptide preparation methods, chemical instruments and methods, etc., can solve the unsatisfactory problems of globulin

Inactive Publication Date: 2010-08-11
SEBIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, to date, separation of albumin and alpha by capillary electrophoresis 1 Globulin is not quite satisfactory

Method used

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  • Method for separating proteins by capillary electrophoresis and buffer compositions for capillary electrophoresis
  • Method for separating proteins by capillary electrophoresis and buffer compositions for capillary electrophoresis
  • Method for separating proteins by capillary electrophoresis and buffer compositions for capillary electrophoresis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1 (comparison)

[0063] The glycine buffer system was prepared as described above. Normal serum was analyzed.

[0064] Electrophoresis was performed using Method A above.

[0065] from figure 1 It can be seen that the obtained electrophoretic spectrum presents 5 continuous peaks, which are read from left to right and belong to γ, β, α respectively. 2 、α 1 Globulin and albumin components.

Embodiment 2

[0067] Octylate was added to the buffer system of Example 1 at a concentration of 2.5 mM.

[0068] Electrophoresis was performed as described in Example 1.

[0069] from figure 2 It can be seen that the obtained electrophoretic spectrum presents five continuous peaks, belonging to γ, β, α 2 、α 1 Globulin and albumin components. After comparing with the result of Example 1, it is shown that in two components-α 1 Separation between globulin and albumin was greatly improved and return to baseline was improved.

Embodiment 3

[0070] Embodiment 3 (comparison)

[0071] According to the steps of Example 1, the buffer system is the borate buffer solution prepared as described above.

[0072] Electrophoresis was performed as described in Example 1.

[0073] from image 3 It can be seen that the obtained electrophoretic spectrum presents 6 continuous peaks, which belong to γ ​​and β respectively. 2 , β 1 、α 2 、α 1 Globulin and albumin components.

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Abstract

The invention relates to a method for capillary electrophoresis in free solution with an alkaline pH, for analysing biological samples comprising protein constituents. The method is characterized in that it comprises at least one step whereby the sample is introduced into a capillary tube containing an analysis buffer. The analysis buffer also comprises at least one additive capable of hydrophobic interaction with one or more protein constituents and capable of providing the protein constituent(s) with one or more negative charges and of altering the electrophoretic mobility thereof. The invention also relates to analysis buffer compositions for carrying out the method.

Description

[0001] This application is a divisional application filed on January 18, 2002, with application number 02800134.6, and the title of the invention is "Method for Separating Proteins by Capillary Electrophoresis and Buffer Composition for Capillary Electrophoresis". field of invention [0002] The present invention relates to a method for separating proteins and peptides by capillary electrophoresis and a buffer composition containing an additive for such separation. Background of the invention [0003] In the analysis of proteins in biological fluids such as serum for diagnostic purposes, in particular the proteins are often separated by electrophoresis, or gel electrophoresis or capillary electrophoresis. One advantage of capillary electrophoresis is that only minute amounts of biological fluid are required for analysis. Also, since high voltages can be used and the sample is not heated to high temperatures during separation, separations can be performed very quickly using t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N33/483C07K1/26
CPCG01N27/44747
Inventor G·诺瓦德耶F·罗伯特
Owner SEBIA