Method for measuring phosphorus content of polysaccharide phosphate ester

Abstract

The invention discloses a method for measuring the phosphorus content of polysaccharide phosphate ester. In the method, sample solution which is processed is detected by the high-efficiency liquid phase chromatography, and the phosphorus content of the detected polysaccharide sample is calculated by preparing gradient standard solution by using through detection, making a standard curve and establishing a regression equation. The method has the advantages of good compatibility of the detected polysaccharide solution and mobile phase mixed liquid, wide linear range of the standard curve, accurate and reliable detection result, simplification of detection steps, measurement time conservation, low measurement cost and easy popularization and implementation. The method overcomes the defects of complicated operation, serious impurity interference, unsatisfactory accuracy and sensitivity, narrow linear range and the like in the conventional process of measuring the phosphorus content.

Description

technical field [0001] The invention relates to a method for determining phosphorus content, in particular to a method for determining phosphorus content in polysaccharide phosphate. Background technique [0002] Polysaccharide phosphate is an important class of polysaccharide derivatives. It introduces phosphate groups into sugar groups, which makes some inactive sugar compounds active, and can improve the biological activity of some polysaccharides. Polysaccharide phosphate includes various polysaccharide phosphate derivatives isolated from nature, such as a polysaccharide phosphate composed of glucose, glycerol and phosphate isolated from Enterococcus faecalis, and mannan isolated from Pichia pastoris Phosphate esters; and various synthetic and semi-synthetic polysaccharide phosphate esters, such as konjac glucomannan phosphate, dextran phosphate, etc. Polysaccharide phosphates have a wide range of biological properties, including antiviral, antitumor, antibacterial, imm...

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Problems solved by technology

Chinese patent CN 1635366A disclosed on July 6, 2005 "a method for detecting phosphorus using a low-resolution inductively coupled plasma mass spectrometer". This method indirectly measures the concentration of phosphorus by measuring the concentration of phosphorus oxides, but Its testing costs are too high to be widely used

Chinese patent CN 1296173A disclosed on May 23, 2001 "the determination method of the content of inorganic phosphorus in the phosphorus-containing antistatic agent", the inorganic phosphorus in the antistatic agent is extracted with saturated sodium nitrate, after digestion, use phosphomolybdenum yellow at 420nm colorimetric determination, but in this method, the processing method is only suitable for the target sample, the applicable range is small, and the detection limit and linear range of the determination are not clearly defined, resulting in a narrow application range

The literature "Research on the Process Conditions of Lentinan Phosphorylation Modification" ("Food and Fermentation Industry", 2007, 33 (12) 64-67) reported the determination method of phosphorus content in lentinan phosphate, and the sample was processed by ashing method. The processing time is long, the process is cumbersome, and the whole process is mainly based on subjective judgment, which leads to inaccurate measurement results

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