Method for measuring phosphorus content of polysaccharide phosphate ester

A technology of phosphorus content and phosphate ester, which is applied in the field of determination of phosphorus content, can solve the problems of undefined detection limit and linear range, inaccurate measurement results, long processing time, etc., and achieve accurate and reliable detection results and good compatibility , the effect of measuring low cost

Inactive Publication Date: 2010-08-11
TIANSHUI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Chinese patent CN 1635366A disclosed on July 6, 2005 "a method for detecting phosphorus using a low-resolution inductively coupled plasma mass spectrometer". This method indirectly measures the concentration of phosphorus by measuring the concentration of phosphorus oxides, but Its testing costs are too high to be widely used
Chinese patent CN 1296173A disclosed on May 23, 2001 "the determination method of the content of inorganic phosphorus in the phosphorus-containing antistatic agent", the inorganic phosphorus in the antistatic agent is extracted with saturated sodium nitrate, after digestion, use phosphomolybdenum yellow at 420nm colorimetric determination, but

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  • Method for measuring phosphorus content of polysaccharide phosphate ester
  • Method for measuring phosphorus content of polysaccharide phosphate ester
  • Method for measuring phosphorus content of polysaccharide phosphate ester

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[0036] Example 1

[0037] The method of the present invention for determining the phosphorus content in polysaccharide phosphate comprises the following steps:

[0038] (1) Draw the standard curve and establish the regression equation:

[0039] Weigh 14.32g KH 2 PO 4 Prepare phosphate (PO) in a 100ml volumetric flask. 4 3- ) The mother liquor with a concentration of 100 mg / ml is then sequentially diluted into a phosphate gradient standard solution of 0.05, 0.1, 0.5, 1, 5, and 10 mg / ml. Take this gradient standard solution and inject it into high performance liquid chromatography for detection. Each injection volume is 20ul, and each sample is measured three times. The average value of the peak area is the ordinate (Y) and the concentration of the phosphate gradient standard solution is the abscissa (X) Draw a standard curve ( figure 1 ) And establish the regression equation Y=2.2246X+0.0888, R 2 =0.9992;

[0040] (2) Sample processing:

[0041] Accurately weigh 2 mg of the polysaccha...

Example Embodiment

[0044] Example 2

[0045] The method of the present invention for determining the phosphorus content in polysaccharide phosphate comprises the following steps:

[0046] (1) Draw a standard curve and establish a regression equation:

[0047] Weigh 14.32g KH 2 PO 4 Prepare phosphate (PO) in a 100ml volumetric flask. 4 3- ) The mother liquor with a concentration of 100 mg / ml is then sequentially diluted into a phosphate gradient standard solution of 0.05, 0.1, 0.5, 1, 5, and 10 mg / ml. Take this gradient standard solution and inject it into high performance liquid chromatography for detection. Each injection volume is 20ul, and each sample is measured three times. The average peak area is the ordinate (Y) and the phosphate gradient standard solution is the abscissa (X) concentration. Draw a standard curve ( figure 2 ) And establish the regression equation Y=2.2207X+0.0821, R 2 =0.9994;

[0048] (2) Sample processing:

[0049] Accurately weigh 2mg of the dry matter sample to be tested, ad...

Example Embodiment

[0052] Example 3

[0053] The method of the present invention for determining the phosphorus content in polysaccharide phosphate comprises the following steps:

[0054] (1) Draw a standard curve and establish a regression equation:

[0055] Weigh 14.32g KH 2 PO 4 Prepare phosphate (PO) in a 100ml volumetric flask. 4 3- ) The mother liquor with a concentration of 100 mg / ml is then sequentially diluted into a phosphate gradient standard solution of 0.05, 0.1, 0.5, 1, 5, and 10 mg / ml. Take this gradient standard solution and inject it into high performance liquid chromatography for detection. Each injection volume is 20ul, and each sample is measured three times. The average value of the peak area is the ordinate (Y) and the concentration of the phosphate gradient standard solution is the abscissa (X) Draw a standard curve ( image 3 ) And establish the regression equation Y=2.2223X+0.0925, R 2 =0.9991;

[0056] (2) Sample processing:

[0057] Accurately weigh 2mg of the dry matter sampl...

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Abstract

The invention discloses a method for measuring the phosphorus content of polysaccharide phosphate ester. In the method, sample solution which is processed is detected by the high-efficiency liquid phase chromatography, and the phosphorus content of the detected polysaccharide sample is calculated by preparing gradient standard solution by using through detection, making a standard curve and establishing a regression equation. The method has the advantages of good compatibility of the detected polysaccharide solution and mobile phase mixed liquid, wide linear range of the standard curve, accurate and reliable detection result, simplification of detection steps, measurement time conservation, low measurement cost and easy popularization and implementation. The method overcomes the defects of complicated operation, serious impurity interference, unsatisfactory accuracy and sensitivity, narrow linear range and the like in the conventional process of measuring the phosphorus content.

Description

technical field [0001] The invention relates to a method for determining phosphorus content, in particular to a method for determining phosphorus content in polysaccharide phosphate. Background technique [0002] Polysaccharide phosphate is an important class of polysaccharide derivatives. It introduces phosphate groups into sugar groups, which makes some inactive sugar compounds active, and can improve the biological activity of some polysaccharides. Polysaccharide phosphate includes various polysaccharide phosphate derivatives isolated from nature, such as a polysaccharide phosphate composed of glucose, glycerol and phosphate isolated from Enterococcus faecalis, and mannan isolated from Pichia pastoris Phosphate esters; and various synthetic and semi-synthetic polysaccharide phosphate esters, such as konjac glucomannan phosphate, dextran phosphate, etc. Polysaccharide phosphates have a wide range of biological properties, including antiviral, antitumor, antibacterial, imm...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N1/28
Inventor 张继杨小舟朱银荣彭小凤付丽娟
Owner TIANSHUI NORMAL UNIV
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