Protein crystallization screening 96 condition kit using polyethylene glycol as precipitator

Abstract

The invention discloses a protein crystallization screening 96 condition kit using polyethylene glycol as a precipitator and aims to solve the technical problem existing in the conventional 96 condition screening kit that the success rate of protein crystallization condition screening is low. The PEG adopted in the technical scheme is the top eight types PEG which are most frequently used according to the statistical data of a BMCD database rather than selected randomly, so that the crystallization success rate of biological macromolecules is obviously improved. For example, the success rate of canavaline is increased from 4.2 percent in background technology to 81.2 percent, the success rate of lysozyme is increased from 29.2 percent in background technology to 46.9 percent, the success rate of protease K is increased from 58.3 percent in background technology to 66.7 percent, and the success rate of catalase is increased from 15.6 percent in background technology to 30.2 percent. In addition, the kit uses the PEG as the precipitator, a few types of reagents are used, and compared with the background technology kit, the kit of the invention is lower in cost.

Description

technical field

[0001] The invention relates to a 96-condition kit for protein crystallization screening, in particular to a 96-condition kit for protein crystallization screening using polyethylene glycol (PEG) with different molecular weights as precipitation agents. Background technique

[0002] Hampton Research's public name is Index TM The specific composition of the 96-condition screening kit is as follows, including three parts, precipitant, salt, and buffer. The precipitant mainly includes ammonium salt, sodium salt, magnesium salt, phosphate, formate, organic matter, polymer, etc., and its concentration is relatively high, about 2M; the salt mainly includes sodium salt, magnesium salt, calcium salt, sulfate , acetate, tartrate, etc., the concentration is low, below 1M; the buffer solution includes 6 kinds, the concentration is 0.1M, and the pH value ranges from 3 to 9.

[0003] Table 1: Index TM 96 condition ingredient list

[0004] serial number ...