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Stenotrophomonas capable of improving herbicide resistance in soybeans, method for preparing same and application thereof

A soybean and resistance technology, applied in the field of Stenotrophomonas and its preparation, can solve the problems of affecting the normal growth of sensitive crops, long persistence period, and great harm of leguminous crops, etc.

Inactive Publication Date: 2010-09-01
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Because atrazine is difficult to degrade and has a long persistence period in the soil, it is easy to affect the normal growth of sensitive crops, especially for leguminous crops.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Obtaining of Stenotrophomonas maltophilia Sneb 42

[0021] Take 1g of soybean field soil, add 100ml of sterile water, take 200μl, add 20ml of sterilized NA liquid medium cooled to below 35°C, cultivate at 25°C and wait for colonies to grow. Colonies of Stenotrophomonas maltophilia were preserved separately for multiplication and physiological and biochemical assays, and those whose culture properties conformed to the characteristics of Stenotrophomonas maltophilia were preserved for future use.

Embodiment 2

[0022] Example 2: Cultivation of Stenotrophomonas maltophilia Sneb 42

[0023] Firstly, the Stenotrophomonas maltophilia Sneb 42 strain was fermented and cultivated, and then the metabolites after fermentation were tested for soybean-inducing activity to determine their inducing effect on soybean.

[0024] Inoculate the bacteria of Stenotrophomonas maltophilia Sneb 42 on the test tube agar medium, the formula of the medium is NA medium, that is, the ingredients: 3g of beef extract, 10g of peptone, 1g of yeast extract, sucrose 10g, 20g agar, add water to 1000mL. Incubate at 25°C for 10 days to obtain test tube species.

[0025] Test tube seed is inoculated in the liquid culture medium of 250mL triangular flask (every bottle 100mL) again, and medium formula is: by weight percentage, containing beef extract 0.2%, peptone 1%, yeast extract 0.5%, sucrose 1%, The remainder was distilled water with a pH of 8. Cultivate at 25°C, shaker speed at 180r min -1 1. After 48 hours of fer...

Embodiment 3

[0026] Example 3: Fermentation of Stenotrophomonas maltophilia Sneb 42

[0027] Basically the same as Example 1, the difference is that the liquid-cultured strains were inoculated into a 1000L fermenter for fermentation, cultured at 25° C., pH 8, and fermented for 48 hours.

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Abstract

The invention relates to stenotrophomonas maltophilia sneb 42, which is capable of inducing soybeans to generate atrazine resistance when used for treating soybean seeds, a method for preparing the same and application thereof, and belongs to the technical field of microbial pesticides. The bacterial strain of sneb 42 was preserved in the General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on February 1st, 2010. The preservation number is CGMCC No. 3620. The stenotrophomonas is prepared by liquid fermentation culture, and when metabolites of the stenotrophomonas are used for treating soybean seeds, the soybean seeds can generate the atrazine resistance while germinating. After the soybeans are treated by the metabolites of the stenotrophomonas, the soybeans can be co-planted with gramineae plants such as corns, and all plants can be weeded by using the uniform atrazine herbicide, so that the problem that the weeding problem in the intercropping between corns and soybeans is solved. The stenotrophomonas and the method have promising application and development prospects.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a Stenotrophomonas bacterium which can improve the resistance of soybeans to soybean sensitive herbicides after treating soybean seeds, and its preparation method and application. Background technique: [0002] my country is the hometown of soybeans, with over 80 million mu of soybean planting area and over 16 million tons of soybean output per year. With the transfer of rural land and the expansion of farming scale, manual weeding has become more and more restricted. However, at present, chemical herbicides in soybean fields are more harmful to corn, and herbicides in corn fields are also more harmful to soybeans, resulting in Intercropping of corn and soybean is difficult to realize. Therefore, if the microbial treatment of soybean seeds can make soybeans resistant to soybean-sensitive cornfield herbicides, the problem of soybean and corn mixed planti...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/01
Inventor 段玉玺陈立杰朱晓峰王媛媛罗在全
Owner SHENYANG AGRI UNIV
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