Three-stage trapping method of cigarette mainstream smoke for smoke toxicity detection

A technology for the detection of mainstream smoke and toxicity of cigarettes, applied in sampling devices and other directions, can solve problems such as undiscovered public reports, and achieve the effect of improving accuracy and scientificity, improving comprehensiveness and capture efficiency, and increasing capture level.

Active Publication Date: 2010-09-08
YUNNAN RES INST OF TOBACCO SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Three-stage trapping method of cigarette mainstream smoke for smoke toxicity detection
  • Three-stage trapping method of cigarette mainstream smoke for smoke toxicity detection
  • Three-stage trapping method of cigarette mainstream smoke for smoke toxicity detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Three-stage capture method for mainstream cigarette smoke

[0021] Trap attachment was performed conventionally on a semi-automatic rotary smoking machine (Borgwaldt RM20 / cs). The first stage of Cambridge filter trapping is carried out with a 92mm filter; after the first stage of Cambridge filter trapping, connect a glass solution trap placed in an ice-water bath, which contains 15mL of PBS solution, pH The value is 7.2; a glass solution trap placed in an ice-water bath is connected in series with the second-stage PBS solution trap, and 15mL of ethyl acetate is contained in it as the third-stage trap. After the state detection of the instrument is completed, 20 cigarettes are sucked and the mainstream smoke of the cigarettes is collected according to the conventional method.

[0022] After the flue gas is collected, the Cambridge filter is soaked in dimethyl sulfoxide (DMSO), ultrasonicated for 20 minutes, and the total particulate matter (TPM) is extracted,...

Embodiment 2

[0026] Example 2: Optimization of the pH value of the PBS solution used in the second-stage capture

[0027] According to the method of Example 1, PBS solutions with three pH values ​​(pH 7.0, 7.2, 7.4) were selected in the second-stage capture to capture the water-soluble components of the gaseous phase of mainstream cigarette smoke.

[0028] the result shows( figure 2 ), the smoke sample captured by the PBS solution of pH 7.4 among the three pH values ​​has the strongest toxic effect on CHO cells, therefore, the PBS solution of pH 7.4 has the highest capture efficiency for the water-soluble components of the mainstream cigarette smoke gas phase .

Embodiment 3

[0029] Embodiment three: the selection of the organic solvent used in the 3rd capture

[0030] According to the method of Example 1, three kinds of organic solvents were selected in the third-level capture: ethyl acetate, 70% glycerin and acetone to capture the organic phase components of the mainstream cigarette smoke gas phase.

[0031] the result shows( image 3 ), the smoke samples captured by 70% glycerol in the three organic solvents had the greatest toxic effect on CHO cells. Within the volume range used for cytotoxicity testing, 70% glycerol has weaker toxicity to CHO cells among the three organic solvents, and acetone has the greatest toxicity to CHO cells (Table 1). Therefore, 70% glycerol is suitable for capturing the organic phase of the gaseous phase of mainstream cigarette smoke.

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Abstract

The invention relates to a three-stage trapping method of cigarette mainstream smoke for smoke toxicity detection, which belongs to the technical field of cigarette smoke safety evaluation. The three-stage trapping method comprises the following steps: first-stage trapping: Cambridge filter trapping, second-stage trapping: PBS (polybutadiene styrene) solution trapping and third-stage trapping: organic solvent trapping, wherein in the second-stage trapping of the cigarette mainstream smoke, the PBS solution the pH value of which is 7.4 is adopted to trap the water-soluble components of the gas phase matter of the mainstream smoke, and in the third-stage trapping of the cigarette mainstream smoke, 70 percent of glycerol is adopted to trap the organic phase components of the gas phase matter of the mainstream smoke. The invention has the advantages of improving the trapping grade of the cigarette mainstream smoke, increasing the trapping comprehensiveness and the trapping efficiency of the smoke, promoting the accuracy and the scientificity of the smoke toxicity detection and the like.

Description

Technical field: [0001] The invention relates to a three-stage trapping method for cigarette mainstream smoke used for smoke toxicity detection, and belongs to the technical field of cigarette smoke safety evaluation. Background technique: [0002] The safety evaluation of cigarettes and tobacco additives aims to improve the quality and level of cigarettes and be responsible to consumers. Cigarette mainstream smoke capture methods include Cambridge filter, electrostatic deposition, jet impingement, cold trap, solid adsorption and solvent capture. Among them, Cambridge filter trapping and solvent trapping are widely used, and the trapping effect is ideal. Cambridge filters are usually used to capture the particulate phase of mainstream cigarette smoke; solution traps are often used to capture the gaseous phase of mainstream smoke. [0003] At present, the capture of cigarette mainstream smoke in international practice usually adopts the second-level capture method, that is,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/22G01N1/24
Inventor 夭建华米其利李雪梅倪红梅缪明明黄海涛
Owner YUNNAN RES INST OF TOBACCO SCI
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