Method for obtaining eustoma regeneration plant by anther culture

A technology for regenerating plants and eustoma, applied in the biological field, can solve the problems of difficulty in producing eustoma cut flowers, and achieve the effect of reliable technical support and solving the difficulties of cut flowers

Inactive Publication Date: 2010-09-22
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems that F1 hybrids need to be imported and cut flowers of eustoma are difficu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0015] Example 1

[0016] A. The eustoma material used is'Ceremony Orange', take the flower buds with the same length as the calyx and the corolla; send the flower buds to the refrigerator for 4 days at 3℃; take out the flower buds and disinfect them with 75% alcohol for 30 seconds, and then use the quality Sterilize the seeds with 0.2% HgCl2 for 10 minutes, and finally rinse with sterile water 3 times. Use autoclaved filter paper to absorb the water on the flower buds; cut off the flower buds, peel off the anthers, and remove the filaments. : MS+0.5mg / L KT+0.5mg / L 2,4-D, pH 5.5 induction medium, the light intensity is: 1500lx, the light time is: 12 hours / day, the temperature is 20℃, Cultivate for 40 days, take it out, put it on the same induction medium as above until the anthers grow callus, cut the callus, and continue to cultivate the anthers without callus in the same induction medium. Carry out subculture under conditions until callus grows;

[0017] B. Place the callus cul...

Example Embodiment

[0020] Example 2

[0021] A. Choose the eustoma material'Art Peach', take the flower bud with the calyx slightly longer than the corolla; put the flower bud in the refrigerator at 6°C for 2 days; take out the flower bud and sterilize it with 75% alcohol for 60 seconds, and then use the mass concentration Disinfect the flower buds with 0.05% HgCl2 for 20 minutes, and finally rinse with sterile water twice, absorb the water on the flower buds with autoclaved filter paper; cut off the flower buds, peel off the anthers, remove the filaments, and inoculate the anthers : MS+3mg / L KT+3mg / L 2,4-D, pH 6.0 induction medium, light intensity: 2000lx, light time: 8 hours / day, temperature 28℃, culture 20 Day, take it out, put it on the above induction medium until the anthers grow callus, cut the callus, the anthers that have not grown callus continue to be subcultured in the same induction medium under the same culture conditions to Grow callus;

[0022] B. Place the callus subcultured in A o...

Example Embodiment

[0025] Example 3

[0026] A. Choose the eustoma material'Cessna White', take the flower buds whose calyx is slightly longer than the corolla; put the flower buds in the refrigerator at 4℃ for 3 days; the flower buds are disinfected with 75% alcohol by volume for 40 seconds, and then the mass concentration is Sterilize the seeds with 0.1% HgCl2 for 15 minutes, and finally rinse with sterile water 4 times, absorb the water on the flower buds with autoclaved filter paper; cut off the flower buds, peel off the anthers, remove the filaments, and inoculate the anthers in: MS+1mg / L KT+1mg / L 2,4-D, pH 5.8 induction medium, light intensity: 1800lx, light time: 10 hours / day, temperature 25℃, culture for 30 days , Take it out, put it on the above induction medium until the anthers grow callus, cut the callus, the anthers that have not grown callus continue to be subcultured in the same induction medium under the same culture conditions to grow Callus

[0027] B. Place the callus cultured in...

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PUM

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Abstract

The invention provides a method for obtaining eustoma regeneration plant by anther culture, comprising the following steps: selecting materials, carrying out flower bud sterilization, inducing callus, enabling the callus to differentiate and germinate, breeding the regeneration plant, etc. By using the method, the success rate of eustoma genetype induction reaches 100%, healing induction rate reaches 35-100%, callus differentiation rate is 8-23%, and differentiated plant number per callus can reach 28. The eustoma regeneration plant produced by the method can be used as a good parent material for F1 generation seed production.

Description

technical field [0001] The invention relates to a method for obtaining eustoma regenerated plants through anther culture, and belongs to the field of biotechnology. Background technique [0002] Eustoma grandiflorum (Eustoma grandiflorum) is an ornamental plant of Gentianaceae, native to North America. It has a light plant, elegant flower color, unique flower shape, smooth and attractive stem and leaves. It is a high-end cut flower that has become popular in the world in recent years. Its provenance is mainly F1 hybrids imported from abroad, which are expensive and bring many difficulties to the production of eustoma cut flowers. Contents of the invention [0003] In order to solve the problems that F1 hybrids need to be imported and cut flowers of eustoma are difficult to produce, the invention provides a method for obtaining regenerated plants of eustoma through anther culture. The invention can carry out anther culture on eustoma eustoma to produce haploid plants, and ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 周旭红莫锡君桂敏王继华张颢蒋亚莲李绅崇曹桦李金泽
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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