Medicament composition containing tetrandrine, tetrandrine derivatives and histone deacetylase inhibitor and application thereof
A technology of sirtuin and tetrandrine, which is applied in the direction of drug combinations, medical preparations containing active ingredients, antineoplastic drugs, etc., can solve the problems of high toxicity and patient intolerance, so as to reduce the dosage of drugs and improve the curative effect, effect of reducing side effects
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Embodiment 1
[0051] Example 1 The combination of different proportions of Tetrandrine and SAHA synergistically promotes the death of HepG2 cells, see Table 2.
[0052] Table 2
[0053]
[0054]
[0055] In the experiment investigating the cell death of liver cancer cell line HepG2 caused by related compounds, it was found that only a small amount of cell death was observed when 5.0 μM tetrandrine or lower concentration and 1.5 μM SAHA or lower concentration were used alone; When the concentration reaches 10.0 μM tetrandrine and 2.0 μM SAHA, only about 10-20% of the cells die; and when the two are used together at a lower concentration (5.0 μM tetrandrine + 1.5 μM SAHA), there is an obvious synergy 32% of cancer cells died; when the two were combined at the ratio of 10.0 μM tetrandrine + 2.0 μM SAHA, a more significant synergistic effect was produced, resulting in about 85% of cancer cells died.
Embodiment 2
[0056] Example 2 The combination of different proportions of tetrandrine and LBH589 synergistically promotes the death of HepG2 cells, see Table 3.
[0057] table 3
[0058]
[0059]
[0060] In the experiment of investigating the cell death of the liver cancer cell line HepG2 caused by related compounds, it was found that when 5.0 μM tetrandrine, 0.015 μM LBH589 or lower concentrations were used alone, there was only a small amount of cell death; even if the concentration of the single drug was increased to 10.0 μM When tetrandrine and 0.025 μM LBH589, only about 20% of the cells died; and when the two were used together at a lower concentration (5.0 μM tetrandrine + 0.015 μM LBH589), a more obvious synergistic effect was produced, resulting in about 20 % of cancer cells died; when the two were combined at the ratio of 10.0 μM tetrandrine + 0.025 μM LBH589, a more significant synergistic effect was produced, resulting in the death of 91% of cancer cells.
Embodiment 3
[0061] Example 3 The combination of different proportions of tetrandrine and LBH589 synergistically promotes the death of PLC / PRF / 5 cells, see Table 4.
[0062] Table 4
[0063]
[0064] In the experiment of investigating the cell death of liver cancer cell line PLC / PRF / 5 caused by related compounds, it was found that only about 15% of the cells died when 5.0 μM tetrandrine, 0.015 μM LBH589 or lower concentrations were used alone; When the concentration reaches 7.5 μM tetrandrine and 0.025 μM LBH589, only about 20-25% of the cells die; and when the two are used together at a lower concentration (5.0 μM tetrandrine + 0.015 μM LBH589), there is a more obvious synergy 34% of cancer cells died; when the two were combined at the ratio of 7.5 μM tetrandrine + 0.025 μM LBH589, a more significant synergistic effect was produced, resulting in 95% cancer cell death.
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