Method for extracting fresh pupa oil of domestic silkworms by using carbon dioxide supercritical technology

A carbon dioxide and supercritical technology, applied in the direction of fat oil/fat production, fat production, etc., can solve the problems of deep processing of silkworm chrysalis, improve the yield and quality of chrysalis oil, improve labor efficiency, and shorten the production cycle.

Active Publication Date: 2013-02-13
广东省蚕业技术推广中心 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As silkworm cocoons are baked at high temperature, sericin denatures, protein and fat of silkworm chrysalis are denatured, oxidized, decomposed and produce odor, which leads to difficulties in deep processing of silkworm chrysalis

Method used

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  • Method for extracting fresh pupa oil of domestic silkworms by using carbon dioxide supercritical technology
  • Method for extracting fresh pupa oil of domestic silkworms by using carbon dioxide supercritical technology
  • Method for extracting fresh pupa oil of domestic silkworms by using carbon dioxide supercritical technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the obtaining method of fresh pupa, see figure 1 .

[0024] 1. Quick freezing with dry ice to kill pupae: take 10 kg of fresh cocoons of Liangguang No. 2 silkworm variety, and remove secondary cocoons. In the dry ice quick-freezing pupa killing machine, the silkworm cocoons were run at -40°C for 20 minutes to achieve the purpose of pupa killing. From the outlet of the pupa killing machine, the residual dry ice is screened out and put into the cocoon bag.

[0025] 2. Storage of fresh cocoons: put the fresh cocoons after pupa killing into cocoon bags (2.5 kg per bag), use an air pump to remove the air in the bags, and seal them. The dry ice remaining in the cocoon is gasified into carbon dioxide at room temperature, making the cocoon bag in an anaerobic state and avoiding the growth of microorganisms. It can be stored for 1 to 2 months at room temperature.

[0026] 3. Fresh cocoon cooking and silk reeling: The cocoon cooking process is different from dryi...

Embodiment 2

[0029] Embodiment 2: carbon dioxide supercritical extraction fresh pupa oil, see figure 2 .

[0030] 1. Prepare homogenate from fresh pupae: add 10 kg of fresh pupae to 10 kg of 95% alcohol, stir and mash with a homogenizer, 3000 rpm, and the homogenization time depends on the size of the equipment.

[0031] 2. Dehydration of fresh pupae: Soak the homogenate in alcohol for 1 hour to achieve the purpose of dehydration. Centrifuge with a centrifuge at 3000 rpm, and the remaining pupa pulp is precipitated for extraction of pupa oil. The supernatant is alcohol, which is recovered by the alcohol distillation tower, and the residue contains a small amount of pupa oil, which is added to the pupa oil product for purification.

[0032] 3. Carbon dioxide extraction of pupal oil: about 8 kg (fresh weight) of dehydrated pupa pulp is added to the steel cylinder of the extractor.

[0033] Carbon dioxide is stored in steel cylinders with a normal temperature and pressure of 20MPa. Use a...

Embodiment 3

[0039] Example 3: Preparation of linoleic acid and linolenic acid from pupa oil.

[0040] 1. Pretreatment of pupa oil: 5kg of pupa oil, add 0.25kg of acid clay, stir vigorously at 100°C for 30min, inject it into the oil felt filter while it is hot, and use 1.25kg / cm 2 Compressed air pressure filtration, obtain clarified pupa oil 4.5kg.

[0041] 2. Vacuum distillation: add the purified pupa oil into a vacuum distiller, heat with No. 54 spindle oil, rectification conditions: vacuum degree 0.09MPa (equivalent to 0.2mmHg), collect fractions at 196°C to 205°C, and cool to obtain The mixture of linoleic acid (ω-3) and linoleic acid (ω-6) is about 2.5kg, which can be used as a pharmaceutical raw material.

[0042] 3. The quality index of unsaturated fatty acid prepared from pupa oil: Refractive index (η): 1.4550; Acid value: 160; Iodine value: greater than 160; Water content: less than 0.15%; Linoleic acid: linoleic acid is 3: 1 .

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Abstract

The invention discloses a method for extracting fresh pupa oil of domestic silkworms by using a carbon dioxide supercritical technology, which is used for treating silkworm cocoon by using a dry ice (-79 DEG C) quick-freezing method to enable silkworm pupa to keep a natural state. The fresh pupa oil is extracted by using the carbon dioxide supercritical technology and replaces solvents of conventional gasoline and the like, and pupa slag which is formed by extracting the pupa oil is used as raw material for deep processing. The traditional cocoon reeling technology comprises the following steps of: killing the silkworm pupa through baking for 6h-8h at 100-105 DEG C by using a coal stove and drying the silkworm pupa to be a dry cocoon, and storing raw materials for reeling under the normal temperature; because silkworm cocoon silk glue is modified due to high-temperature baking, the proteins and the fats of the silkworm pupa are decomposed and oxidized to generate stink so as to difficultly carry out deep processing. In the invention, fresh pupa is obtained by using a new method for extracting fresh pupa oil of domestic silkworms by using the carbon dioxide supercritical technology based on a storage fresh-keeping method of fresh cocoons of domestic silkworms in a patent number of 20081002877.6, a method for killing pupa by quick-freezing fresh cocoons of domestic silkworms in a patent number of 200810029512.5 and an apparatus for killing pupa by quick-freezing fresh cocoons of domestic silkworms in a patent number of ZL200820050763.7 of the utility model.

Description

technical field [0001] The invention relates to the fields of silkworm cocoon reeling engineering and silkworm chrysalis deep processing, and specifically illustrates a method for supercritically extracting silkworm fresh chrysalis oil by using carbon dioxide. technical background [0002] After the silkworm fresh cocoons are purchased, they are dried in a coal stove (100°C-105°C, 6h-8h) to kill the silkworm chrysalis and dry them into dry cocoons. Stored at room temperature, it is used as raw material for reeling raw silk. Because silkworm cocoons are baked at high temperature, sericin denatures, protein and fat of silkworm chrysalis are denatured, oxidized, decomposed by heat and produce odor, which leads to difficulties in deep processing of silkworm chrysalis. [0003] In the present invention, fresh cocoons are killed by dry ice (-79 DEG C), and carbon dioxide is used to store the fresh cocoons under anaerobic conditions. Fresh pupae are obtained by boiling cocoons an...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C11B1/10C11B1/02
Inventor黄自然邱国祥王先燕胡智明林忠芬李惠华陈芳艳袁永杨庞华兴
Owner广东省蚕业技术推广中心