Method for preparing dual-regulated oncolytic adenovirus for expressed superantigen gene of targeted prostate tumor

Abstract

The invention relates to an expressed superantigen gene of targeted prostate tumor, in particular to a method for preparing dual-regulated oncolytic adenovirus for the expressed superantigen gene of the targeted prostate tumor, and belongs to the technical field of gene therapy. The preparation method comprises the following steps of: by applying the genetic engineering technology, cloning staphylococcus aureus enterotoxin A genes into an oncolytic adenovirus vector so as to limit the proliferation and expression of the genes in prostate tumor cells only and fulfill the aim of killing target prostate tumor cells; and regulating a tumor specific proliferous adenovirus vector by using a prostate specific antigen promoter and a telomerase reverse transcriptase promoter, carrying the staphylococcus aureus enterotoxin A gene fragment, and naming as SG504-SEA. The expressed superantigen gene has the advantages of specific proliferation in tumor cells and high cytotoxic T lymphocyte irritating capacity.

Description

Technical field [0001] The invention relates to a targeted anti-prostate tumor-expressing superantigen gene, in particular to a method for preparing a double-regulated oncolytic adenovirus targeting anti-prostate tumor expressing a superantigen gene, and belongs to the technical field of gene therapy. Background technique [0002] Prostate cancer is one of the most common malignant tumors of the male reproductive system, and its incidence has been increasing year by year in recent years. A large number of survey data show that the incidence of prostate cancer and related deaths in my country are also increasing year by year, and the diagnosis and treatment of prostate cancer has become an important research content of modern medicine. At present, there are many treatment strategies related to prostate cancer gene therapy. Because most treatment methods do not have good specificity and cannot directly target prostate cancer cells, their clinical applications are limited. As we a...

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Problems solved by technology

[0007] Secondly, since the adenovirus used for oncolysis is introduced into the body as an in vitro substance, the body will inevitably produce a defense against the virus, resulting in fever, local injection reactions, flu-like symptoms, leukopenia, thrombocytopenia, abnormal liver and kidney function, Adverse reactions such as hair loss, nausea and vomiting, the appearance of these adverse reactions indicates that the body's own immune response has an effect on the virus, which will reduce the virus titer, and the killing effect of the virus on tumor cells will naturally weaken

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