High performance liquid chromatography method of oxytetracycline residues in soils

Abstract

The invention discloses a high performance liquid chromatography method of oxytetracycline residues in soils, comprising the following steps: (1) preparing oxytetracycline standards solution and extracting buffer solution Na2EDTA-Mcllvaine; (2) extracting oxytetracycline in soil samples; (3) purifying and eluting oxytetracycline solution to be tested to obtain eluent; (4) adding mobile phase after drying the eluent by blowing and carrying out filtration after ultrasonic extraction to obtain filtrate; and (5) determining the content of oxytetracycline in the filtrate by high performance liquid chromatography method. The method is simple and convenient in operation and high in accuracy. The content of oxytetracycline has good linear relation with peak area. The recovery rate, sensitivity, detection limit and relative standard deviation can meet the requirements of residue analysis. Experiments prove that the method can be used for various soils with bigger property differences and the recovery rate, sensitivity and detection limit have better accuracy and precision in various soils.

Description

technical field [0001] The invention belongs to the detection technology of chemical substance residues, in particular to a high performance liquid chromatography detection method of oxytetracycline residues in soil. Background technique [0002] In recent years, with the development of intensive animal husbandry and compound feed industry, tetracyclines, macrolides, penicillins, amino sugars and sulfonamide / trimethoprim antibiotics have been widely used as feed additives. Used in livestock and poultry farming and aquaculture. According to statistics, the annual consumption of antibiotics in the breeding industry in the European Community has reached more than 5,000 tons; the annual consumption of antibiotics in the animal breeding industry in the United States is about 11,000 tons, accounting for about 70% of the total amount of antibiotics; the consumption of antibiotics in my country is about About 600 times that of the United States. Among the antibiotics used, tetracyc...

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Problems solved by technology

There are many types of soil in my country, and its properties are quite different from agricultural products such as livestock, poultry, eggs, meat, et

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