Process for degrading zearalenone in a feed product employing laccase

A technology of zearalenone and products, applied in the field of feed products detoxified by mycotoxin zearalenone

Inactive Publication Date: 2010-12-01
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Production of zearalenone does not appear to occur in significant amounts prior to harvest, bu...

Method used

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  • Process for degrading zearalenone in a feed product employing laccase
  • Process for degrading zearalenone in a feed product employing laccase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Materials and methods

[0062] enzyme

[0063] Laccase from Myceliophthora thermophila (MtL) having the amino acid sequence set forth herein as SEQ ID NO:1.

[0064] Laccase from Polyporus pinsitus (PpL) having the amino acid sequence shown herein as SEQ ID NO:2.

[0065] Laccase from Streptomyces coelicolor (ScL) having the amino acid sequence set forth herein as SEQ ID NO:3.

[0066] mediator

[0067] Methyl syringate (MeS)

[0068] Phenothiazine-10-Propionic Acid (PPT)

[0069] Assay : Reactions were performed in Eppendorf tubes in a volume of 300 μl containing zearalenone 30 μM, mediator 0.2 mM, sodium acetate 100 mM and enzyme 0.1 mg EP / mL. In a control reaction, the enzyme-containing volume was replaced with an equal amount of H 2 O substitute. The reaction was incubated at 37°C for 24 hours, after which the reaction was stopped by adding 600 μL of 100 μM acetonitrile stop solution. The reaction was stored at -20°C until chromatographic analysis.

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PUM

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Abstract

The present invention relates to a method for detoxification of feed products contaminated by the mycotoxin zearalenone.

Description

[0001] related to the sequence listing [0002] This application contains a Sequence Listing in computer readable form. The computer readable form is incorporated herein by reference. Field of Invention [0003] The present invention relates to a method of detoxifying feed products contaminated with the mycotoxin zearalenone. Background of the Invention [0004] Several phytopathogenic and / or post-harvest Fusarium species on cereals produce toxicants of considerable concern to livestock and poultry producers, eg, deoxynivalenol, T-2 toxin, HT- 2 toxins, diacetoxyscirpenol and zearalenone. [0005] Zearalenone is present in a variety of cereal crops worldwide, such as corn, barley, oats, wheat, rye, rice, millet and sorghum. The production of zearalenone does not appear to occur in significant amounts before harvest, but under suitable environmental conditions it is readily produced on corn and small grains in storage. [0006] When grain particles are used in ethanol pro...

Claims

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Application Information

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IPC IPC(8): C12P1/00A23L1/03A23K1/00A23K10/38A23L5/20A23L29/00
CPCA23K1/184A23K1/06C12P1/00A23L1/0153A23K1/1656A23K10/38A23K10/14A23K50/30A23L5/25C12N9/0061Y02P60/87
Inventor 安德斯·维克索-尼尔森伯思·H·索伦森
Owner NOVOZYMES AS
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