Process for extracting pleocidin from fermentation liquor of saccharopolyspora spinosa

A technology for Saccharopolyspora spinosa and spinosyn, which is applied in the field of spinosyn extraction, can solve the problems of being difficult to adapt to large-scale industrial production, high equipment requirements and high cost, and achieves mature equipment development, low labor intensity and product quality. Good results

Inactive Publication Date: 2013-03-27
HUNAN NORMAL UNIVERSITY
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Problems solved by technology

[0006] Although the resin adsorption extraction method has a good effect to a certain extent, such as high purity, the macroporous resin adsorption extraction process currently has small adsorption capacity, difficult desorption, small processing capacity, high equipment requirements, and many processes. It is difficult to adapt to the requirements of large-scale industrial production due to defects such as long time consumption and high cost
[0007] At present, in the prior art, there is no spinosad extraction process with high yield, good purity, low cost, easy operation and convenient industrial production.

Method used

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  • Process for extracting pleocidin from fermentation liquor of saccharopolyspora spinosa
  • Process for extracting pleocidin from fermentation liquor of saccharopolyspora spinosa
  • Process for extracting pleocidin from fermentation liquor of saccharopolyspora spinosa

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Embodiment 1

[0022] (1) Add 5 mol / L NaOH solution to the fermentation broth in the fermenter to adjust the pH of the fermentation broth to 9.5, then heat the fermentation broth to 60°C, heat and stir for 35 minutes; (2) After cooling the fermentation broth to room temperature Add methanol, the volume ratio of fermentation broth to methanol is 1:1.5, stir and extract at 30°C for 8 hours, discharge the material to a plate and frame filter press, filter and collect the filtrate to obtain the extract clear liquid; (3) extract clear The liquid was transferred to a rotary evaporator for vacuum concentration and volatilization to completely remove methanol to obtain a spinosad concentrated solution with a yield of 98.3%; (4) add butyl acetate to the spinosad concentrated solution for extraction, and the extraction conditions were: two-phase volume ratio ( Extraction solvent / spinosyn concentrated solution) = 1:1, the temperature is room temperature 25°C, pH = 9.0, and the extraction time is 10 min;...

Embodiment 2

[0024](1) Add 3 mol / L NaOH solution to the fermentation broth in the fermenter, adjust the pH of the fermentation broth to 10.5, heat the fermentation broth to 65°C, and heat and stir for 45 minutes; (2) After cooling the fermentation broth to room temperature Add methanol, the volume ratio of fermentation broth to methanol is 1:1, stir and extract at 30°C for 12 hours, then discharge the material to a plate and frame filter press, filter and collect the filtrate to obtain the extract clear liquid; (3) extract The supernatant liquid was transferred to a vacuum concentration tank to volatilize and remove methanol to obtain a spinosad concentrated solution with a yield of 98.4%; (4) Add butyl acetate to extract the spinosad concentrated solution, using a CTL70-N centrifugal extractor with a drum diameter of 70 mm, rotation speed 2500 r / min, extraction conditions: volume ratio of two phases (extraction solvent / spinosyn concentrate) = 1:1, temperature 30°C, pH = 8.5; (5) The CTL70...

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Abstract

A process for extracting spinosads from fermentation broth of Saccharopolyspora spinosa, comprising the following steps: (1) pretreatment of fermentation broth; (2) soaking and extracting spinosads in high dielectric constant polar organic solvents, and carrying out Solid-liquid separation, collection of leaching clear liquid; (3) Vacuum concentration, volatilization and removal of high dielectric constant polar solvents to obtain spinosad concentrates; (4) Adding low dielectric constant or high-carbon alcohol extraction solvents for extraction , to obtain the loaded organic phase; (5) add acid water to the loaded organic phase for back extraction, and collect the back extraction phase; (6) volatilize to remove the residual extraction solvent in the back extraction phase, and adjust the pH value to 8.5~11.5 with NaOH solution, Precipitate the spinosyn, filter, wash the spinosyn precipitate with dilute lye for 1 to 3 times, and dry in vacuum to obtain the spinosyn powder. The total yield of the invention can reach more than 80%, and has the advantages of high yield, good quality, low cost, easy industrialization and the like.

Description

Technical field [0001] The present invention involves a process from extracting antibiotics from the fermentation solution, especially the process of extracting multi -bacterin from the extraction method from the extraction method from the spores of spores. Background technique [0002] Sipinosad (SPINOSYNS), also known as spinosyns, and polyromycin. SacCharopolyspora Spinosa ) The secondary metabolites produced by aerobic fermentation are large -ring endone antibiotics. The molecular structure contains a twenty -two yuan lactone ring, a rat lip sugar, and a Fullagamine.It is easy to dissolve in a variety of polar and non -polar organic solvents such as alcohol and ester.After the aerobic fermentation, the spines polymoglacular fermentation liquid contains two types of insecticidal activity, which mainly contain multiple bactericin A (85 ~ 90%) and D (10 ~ 15%), and also contain a small amount of B, C, E, E, F, H, J, K, L and other similar structures are similar.Multi -bactericin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/08C07H1/08
Inventor 夏立秋丁学知余子全杨尉伍维
Owner HUNAN NORMAL UNIVERSITY
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