146 results about "Bactericin" patented technology

This invention refers to biocompatible carbohydrate polymers such as modified polysaccharides (e.g. chitosan, alginate), associated with milk protein (e.g. caseinate and/or whey proteins) designed to carry bioactive agents. The formulations may be used in various delivery systems including beads, tablets, microencapsulating agents and coatings for oral dosage forms, implants for subcutaneous devices and films for topic administration and food protection. These formulations present improved chemical resistance and exert their activity for prolonged time into gastrointestinal tract (GIT) and blood circulation as well as for preserving food qualities over long period. The association of modified chitosan, modified alginate with milk proteins results in a stabilized structure able to control the release of drugs, bacteria, bacteriocines, enzymes, nutraceutics, etc. into enteric, topic or systemic route.

The present invention discloses a method for producing a fermented dairy product, preferably cheese, comprising inoculating milk with lactic acid bacteria capable of producing a Class IIa type bacteriocin; and lactic acid bacteria capable of producing a Class I type bacteriocin.

The invention discloses lactobacillus casei bacteriocin and use thereof in feed. The lactobacillus casei provided by the invention is separated from Tibet plateau traditional fermented yak yogurt, and the produced bacteriocin is obtained by ammonium sulfate precipitation, Sephadex G-100 gel filtration and reversed-phase high-performance liquid chromatography (RP-HPLC). The bacteriocin has thermal and acid stability, can be degraded by protease and has a wide antibacterial spectrum. When the bacteriocin is added into feed, and the growth of bacteria can be inhibited. The bacteriocin has a bright prospect in use as a feed additive.

The invention discloses a bacteriocin and superhigh pressure joint antiseptic preservation technology and an application thereof. The invention provides a bacteriostasis and/or sterilization method for food. The bacteriostasis and/or sterilization method for the food provided by the invention comprises the following steps: adding the bacteriocin enterocin P into the food, and performing superhighpressure treatment on the food added with the bacteriocin enterocin P, wherein the pressure for the superhigh pressure treatment is 100-600 MPa. According to the invention, under the condition of notadding any chemical preservative, the defects of relatively poor bacteriostasis activity of nisin against Gram-negative bacteria and influence of 600 MPa superhigh pressure treatment on the sensory quality of food are overcome by combining the bacteriocin enterocin P with the superhigh pressure technology, and an efficient, environmentally-friendly and safe antiseptic preservation technology is provided for low-temperature meat products.

The invention discloses a bacteriocin crude extract with bacteriostatic action, and a preparation method and application thereof. The preparation method of the bacteriocin crude extract comprises the following steps: (1) inoculating Paenibacillus sp. into a culture medium for culturing Paenibacillus sp., and carrying out shake fermentation and culture to obtain a fermentation liquid; and (2) centrifuging the fermentation liquid obtained in the step (1), taking the precipitate, adding acetone, evenly mixing, leaching, centrifuging, taking the supernatant, and removing acetone. The method solves the problem of narrow bacteriocin sources at present. Compared with the traditional bacteriocin, the bacteriocin crude extract prepared by the method has stabler properties, and thus, has higher food safety. The bacteriocin crude extract can be directly used for food production, thereby achieving the goals of inhibiting putrefying bacteria and prolonging the shelf life of food, and further lowering the application cost.

The invention relates to a lactococcus garviea bacteriocin, a preparing method thereof and an application thereof. The high-yield actococcus garviea bacteriocin can be obtained in the mode that lactococcus garviea is subjected to fermented cultivation, and then the bacteriocin in the cultivating-fermenting liquid is extracted with a pH adsorption method; more than 800 mg of the lactococcus garviea bacteriocin can be prepared through 1 L of the fermenting liquid. The lactococcus garviea bacteriocin has quite high broad-spectrum bacteriostasis capacity and has a quite high inhibiting effect on some gram negative bacteria, some gram positive bacteria, yeast and mould causing human diseases and food spoilage. The lactococcus garviea bacteriocin has the effect of treating and preventing acute and chronic enteritis.

The invention provides multi-effect bacillus subtilis for high yield of immune polysaccharide and bacteriocin and application. The preservation number of the bacillus subtilis provided by the invention is CGMCC NO.20463. The strain has excellent extracellular immune polysaccharide production capability; after fermentation by a conventional method, fermentation liquor is purified, and the yield ofthe exopolysaccharide is as high as 8203.64 mg/L; and the strain has excellent bacteriocin production capacity and endotoxin digestion capacity, and can effectively inhibit pathogenic or harmful bacteria such as salmonella, escherichia coli, staphylococcus aureus, listeria monocytogenes, sarcina lutea, streptococcus, clostridium perfringens and the like. The bacillus subtilis disclosed by the invention can be used as a food additive or a feed additive, has good immunoregulation, antibacterial, antiviral, anti-inflammatory and antioxidant capacities, and has a wide application prospect in the aspects of disease resistance, disease prevention and livestock and poultry antibiotic substitution.

The invention discloses a mutation breeding method of lactobacillus plantarum and application of a strain of the lactobacillus plantarum. The collection number of the lactobacillus plantarum in China general microbiological culture collection center is CGMCC No 5297. The lactobacillus plantarum can generate bacteriocin which has a better inhibiting effect on multiple gram-positive bacteria and especially has great a high inhibiting effect on listeria monocytogenes, and the bacteriocin has the advantages of better heat stability, acid stability and the like and can be applied as a food additive and a feed preservative agent which have wide application prospect.

The invention discloses a natural composite preservative special for chilled fresh meat. The invention provides Lactobacillus plantarum b-2 collected under CGMCC No. 13413. The invention also claims bacteriocin produced by the Lactobacillus plantarum b-2. The bacteriocin produced by the Lactobacillus plantarum b-2 is blended with ginger juice to prepare the natural composite preservative; the natural composite preservative is good in safety and zero in toxicity, has antibacterial, anti-oxidization and health functions, can be added to prevent chilled fresh meat from spoiling, significantly extend the shelf life of chilled fresh meat and improve the safety of the chilled fresh meat, may extend the shelf of chilled fresh meat for 4 days, and has a promising application prospect.

The invention discloses a bacteriocin bacteriostatic fresh-keeping biological composite film. The biological composite film is prepared by preparing a film matrix from 94-97wt% of polylactic acid and 3-6wt% of sawdust particles and adsorbing a trace amount of bacteriocin on the film surface. A manufacturing method comprises the following steps of stirring and mixing the pretreated sawdust particles and polylactic acid, extruding and blowing the film; carrying out dry heat treatment on the biological composite film and implanting the bacteriocin into the film by virtue of a diffusion coating method. The manufacturing method is simple and low in production cost, the bacteriocin can be effectively adsorbed on the surface of the biological composite film and the industrial production can be realized. The prepared bacteriocin bacteriostatic fresh-keeping biological composite film has the advantages of stronger effects on inhibiting activities of multiple food-borne pathogenic bacteria and food spoilage bacteria, very low solubility in water and complete biodegradability and is especially suitable for fresh-keeping preservation of fresh meat and other high-moisture foods.

The invention discloses novel effective cerecidin and an application thereof. The cerecidin disclosed by the invention is a modified polypeptide. A synthetic method of the cerecidin disclosed by the invention is simpler compared with the conventional method. Meanwhile, the synthesized cerecidin has relatively high antibacterial activity, the antibacterial effect of the cerecidin for resisting vancomycin enterococcus faecalis V583(VRE) is better than that of nisin, and under a relatively wide pH condition and a high temperature, the cerecidin is relatively stable.

The invention provides a lactobacillus paracasei producing bacteriocin and an application thereof. The present invention relates to a Lactobacillus paracasei S20 strain with a preservation number of CGMCC NO. 17120, The strain is isolated from a Tibetan traditional fermented food-milk block. A crude bacteriocin extract is obtained by extracting from a MRS fermentation supernatant of the strain byusing ammonium sulfate grading deposition. The experiment proves that the bacterial extract produced by the strain has a strong inhibitory effect against Staphylococcus aureus and Escherichia coli, and the minimum inhibitory concentration measured by a double dilution method is 312 and 625 [mu]g/mL, respectively. The bacteriocin produced by the strain can be hydrolyzed by proteases such as neutralprotease and trypsin, and does not accumulate in the body. The Lactobacillus paracasei and its bacteriocin screened by the invention have broad application prospects in fermented foods.

The invention relates to a composite lactobacillus preparation capable of resisting helicobacter pylori, reducing blood sugar, conditioning intestines and stomach and enhancing immunity, a preparation method and application thereof. Lactobacillus plantarum MiuCure-01, lactobacillus acidophilus MiuLac-01, lactobacillus casei MiaoTech-01, lactobacillus rhamnosus MiaoTech-11 and streptococcus thermophilus MiaoPro-01 are adopted as raw materials. the invention is prepared by a novel process combining mixed bacteria inoculation, liquid culture, solid-liquid separation, solid culture and the like, the compound lactobacillus with higher activity is obtained, antibacterial substances such as bacteriocin and organic acid generated in the solid culture process are reserved, and the invention has the functions of resisting helicobacter pylori and coordinating intestines and stomach; and various metabolites capable of reducing blood sugar and enhancing immunity can be produced. The solid culture is low in moisture content and easy to dry, stress death of the compound lactobacillus in the drying period can be reduced, and the activity of the compound lactobacillus in the product can be greatly improved by combining proper granulation and coating procedures. The produced composite lactobacillus probiotic preparation has the effects of resisting helicobacter pylori, reducing blood sugar, coordinating intestines and stomach, improving immunity and the like.