114 results about "Bactericin" patented technology

Provided herein are fusion polypeptides comprising an amino acid sequence of a bacteriocin, the amino acid sequence of the bacteriocin being a cleavage intein both on both the N-terminus and on both the C-terminus that cyclizes the bacteriocin. Also provided are nucleic acids and genetic vectors encoding the fusion polypeptides, as well as microbial cells genetically engineered with the nucleic acids or genetic vectors. Also provided are methods of making the cyclic bacteriocin, methods of screening using a library of nucleic acids or genetic vectors encoding the fusion polypeptides, and methods of controlling growth of organisms using the cyclic bacteriocin made by the methods provided herein.

The invention belongs to the technical field of biology, and provides enterococcus faecalis, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.29087. The enterococcus faecalis is characterized in that the enterococcus faecalis can secrete bacteriocin for inhibiting vibrio parahaemolyticus, staphylococcus aureus and escherichia coli. The invention also provides the enterococcus faecalis and application thereof in an aquatic product fermented feed and a laying hen premix, and the enterococcus faecalis can effectively improve the utilization rate of the feed and the growth and production performance of animals.

The invention relates to the technical field of microbial fermentation, in particular to a preparation method of a glucose fermentation product with bacteriostatic activity, which comprises the following steps: (1) inoculating a lactococcus lactis subsp. Lactis seed solution into a glucose-containing fermentation culture solution, and fermenting to obtain a first fermentation solution; (2) inoculating a pediococcus acidilactici seed solution into the first fermentation liquor, supplementing glucose, and fermenting to obtain second fermentation liquor; and (3) adjusting the pH value of the second fermentation liquor to be acidic, filtering, concentrating, and spray-drying to obtain the glucose fermentation product. According to the invention, the characteristic that organic acids and bacteriocin generated by lactococcus lactis subsp. Lactis and pediococcus acidilactici are different in types is fully utilized, metabolites of the two bacteria are obtained at the same time by adopting a sequential sequence type fermentation mode, and the metabolites of the lactococcus lactis subsp. Lactis are converted by pediococcus acidilactici, so that a new substance with bacteriostatic activity is obtained; the antibacterial activity is improved. Meanwhile, the problem of nutrition competition caused by mixed culture of the two bacteria is avoided, and the fermentation efficiency is improved.

The invention discloses a bacterial inhibitor for sea bass preservation, the bacterial inhibitor is bacteriocin generated by fermentation of lactobacillus plantarum L13 (the preservation number is CGMCC NO.27398), the bacteriocin comprises active polypeptide xylAB, the amino acid sequence of the xylAB is LLSSLATTGAIALGATAAKA, the preservation number of the bacterial inhibitor is CGMCC NO.27398, the preservation number of the bacterial inhibitor is CGMCC NO.27398, and the preservation number of the bacterial inhibitor is CGMCC NO.27398. The fresh-keeping agent prepared by the invention has a good fresh-keeping effect, can prolong the fresh-keeping period, and has huge potential as a natural food preservative in the food industry.

The invention provides a lactobacillus pentosus and a preparation method and application of bacteriocin of the lactobacillus pentosus. The name of the strain is lactobacillus pentosus YCL31, and the strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 25, 2025, the preservation address is the 5th floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 66589. The bacteriocin separated and purified from the metabolite of the bacterium can destroy the morphological structure of the vibrio parahemolyticus and inhibit the toxicity of the vibrio parahemolyticus, can also achieve the effect of remarkably inactivating the vibrio parahemolyticus in a metapenaeus ensis system, can effectively delay the spoilage of the metapenaeus ensis, and has a good application prospect as a bacteriostatic agent or preservative. Therefore, development and application of the novel bacteriocin have important reference value in related fields.

The present invention provides a Pediococcus pentosaceus ( Pediococcus pentosus ) strain ZPP083, which is deposited with the General Microbiology Center of the China National Center for Microbiological Culture Collection under the CGMCC No. 31944. The Pediococcus pentosaceus provided by the present invention has a strong antibacterial effect and produces a novel Class IIa bacteriocin with acid, heat, and protease resistance. The present invention also constructs a genetically engineered bacterium using this bacteriocin, increasing its expression.

This application relates to the field of food microbiology, and specifically discloses a Lactobacillus plantarum capable of producing 5-methyltetrahydrofolic acid, its fermentation method and application. A Lactobacillus plantarum inm30-LP capable of producing 5-methyltetrahydrofolic acid, with a preservation date of December 26, 2022, and the preservation unit is the General Microbiology Center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No. 26278. This application is based on the milk curd in the Gannan Tibetan area as the food source, isolates probiotics, and screens out Lactobacillus plantarum with high folic acid production from them. Moreover, the strain has obvious advantages over other lactic acid bacteria in terms of acid resistance and bile salt resistance, is suitable for growing in the gastrointestinal environment, has the ability to proliferate, has no antibiotic resistance, has antibacterial activity, and can produce bacteriocin.

The invention provides a Lactobacillus casei GG002, a bacteriocin solution of the Lactobacillus casei GG002, freeze-dried powder of the Lactobacillus casei GG002 and application of the bacteriocin solution and the freeze-dried powder of the Lactobacillus casei GG002 to treatment of oral ulcer, and belongs to the technical field of microorganisms. The invention finds that the lactobacillus casei GG002 can inhibit streptococcus mutans, fusobacterium nucleatum and porphyromonas gingivalis, and has the effect of treating oral ulcer. The invention further provides a bacteriocin solution, freeze-dried powder and metagen of the casei casei GG002, and the bacteriocin solution, the freeze-dried powder and the metagen can be used for effectively treating the oral ulcer.

The invention discloses a bacterial co-culture metabolite for improving the yield of bacteriocin and application thereof, and is characterized in that the bacterial co-culture metabolite comprises at least one of L-chorolactone, N-acetyl aspartic acid, butyryl trihexyl citrate, N-acetyl-L-glutamic acid and ascorbic acid; the invention also provides an application of the bacterial co-culture metabolite in preparation of a fermentation medium for improving the yield of bacteriocin, and the fermentation medium is prepared by adding 0.3% by mass of L-chorolactone, 0.1% by mass of N-acetylaspartic acid, 0.1% by mass of butyryl trihexyl citrate, 0.1% by mass of L-aspartic acid, 0.1% by mass of N-acetylaspartic acid, 0.1% by mass of citric acid butyryl trihexyl ester, 0.1% by The culture medium is an MRS liquid culture medium containing 0.2% of N-acetyl-L-glutamic acid and 0.2% of ascorbic acid or an MRS liquid culture medium containing 0.1% of N-acetyl aspartic acid, 0.1% of L-chorolactone, 0.3% of ascorbic acid and 0.3% of butyryl trihexyl citrate in mass concentration. The culture medium has the advantages that the yield of bacteriocin of co-culture and single bacteria is increased.

The present invention provides Lactobacillus casei ( Lacticaseibacillus casei ) Application of GG002 and its bacteriocin solution, lyophilized powder and postbiotics in treating oral ulcers, belonging to the field of microbial technology. The present invention finds that Lactobacillus casei GG002 can inhibit Streptococcus mutans, Fusobacterium nucleatum and Porphyromonas gingivalis, and has the effect of treating oral ulcers. The present invention further provides a bacteriocin solution, lyophilized powder and postbiotics of Lactobacillus casei GG002, which can effectively treat oral ulcers.

The invention relates to a lactobacillus plantarum LAS3 strain and an application of the lactobacillus plantarum LAS3 strain, and belongs to the technical field of microorganisms. The lactobacillus plantarum LAS3 disclosed by the invention is preserved in Guangdong Microbial Culture Collection Center, and the preservation number of the lactobacillus plantarum LAS3 is GDMCC NO.66440. The strain is separated, purified and screened from Guizhou sour fish, and experiments show that the strain has excellent pH tolerance and sodium chloride tolerance; fermentation liquor of the strain has a good antibacterial effect on multiple common pathogenic bacteria such as pseudomonas aeruginosa, burkholderia cepacia, pseudomonas putida, staphylococcus aureus, bacillus subtilis and candida albicans, and bacteriocin generated by the strain has excellent antibacterial and preservative effects and also has excellent thermal stability and pH stability. The antibacterial preservative can be used as an antibacterial preservative raw material, is applied to food, health care products or daily chemical products, and has wide application prospects and important conversion research values.

The present invention relates to the technical field of antiseptic and fresh-keeping, and in particular to a novel biological preservative and its application. Specifically, the present invention mixes pectin and extracellular vesicles of Lactobacillus plantarum LPL-1 in proportion to prepare a biological preservative and fresh-keeping agent. The biological preservative and fresh-keeping agent provided by the present invention can effectively inhibit Gram-positive pathogens such as Listeria monocytogenes and Staphylococcus aureus and Gram-negative pathogens such as Pseudomonas; it can be used for food preservation and antiseptic, is a natural biological preservative, and can effectively extend the shelf life. Compared with traditional lactic acid bacteria bacteriocins, the extracellular vesicles produced by Lactobacillus plantarum can be used as a natural carrier of bacteriocin substances, and more bacteriocin substances can be enriched in the extracellular vesicles. The structure and function of the extracellular vesicles are more stable, not affected by external factors, and easier to carry out large-scale industrial production.

The invention discloses antibacterial bone cement and application thereof, and belongs to the technical field of medical materials. The antibacterial bone cement comprises a solid phase agent and a liquid phase agent, raw materials of the solid phase agent and / or the liquid phase agent comprise bacteriocin containing an olefin structure. The antibacterial peptide with antibacterial activity is added to obtain the novel bone cement, the novel bone cement has good antibacterial property, mechanical property and biocompatibility, and the problem of explosive release of antibacterial drugs in existing PMMA bone cement is solved.

The invention relates to the technical field of microorganism application, and particularly discloses lactobacillus plantarum SY-25 and bacteriocin and application of the lactobacillus plantarum SY-25, the lactobacillus plantarum SY-25 is preserved in China Center for Type Culture Collection on March 31, 2025, the preservation number is CCTCC NO: M 2025635, and the lactobacillus plantarum SY-25 is classified and named as lactobacillus plantarum SY-25. The lactobacillus plantarum SY-25 provided by the invention has an antibacterial broad spectrum property.

The invention belongs to the technical field of food processing, and particularly discloses a preparation process of natural preservative chicken soup based on lactobacillus fermentation. The process mainly comprises the following steps: decocting chicken and water according to a specific ratio to obtain basic chicken soup; the preparation method comprises the following steps: adding lactose, a yeast extract and the like as fermentation substrates, sterilizing, and inoculating a compound lactic acid bacteria starter consisting of lactobacillus plantarum and pediococcus pentosaceus to carry out anaerobic directional fermentation until the pH value is reduced to 4.0-4.6; and after fermentation is completed, adding natural preservative enhancers such as lactoferrin, and finally, homogenizing and filling to obtain the product. Natural preservation and flavor improvement of the chicken soup are synchronously achieved through a biological fermentation method, the obtained product is high in bacteriocin yield and remarkable in pathogenic bacterium inhibition effect, long-acting preservation can be achieved without adding chemical preservatives, meanwhile, the content of free amino acid and umami substances is greatly increased, and the fresh-keeping effect is good. The method is suitable for industrial production of high-quality concentrated chicken soup seasoning and liquid soup base.

It can be useful to regulate the growth of microbial cells. Some embodiments herein provide genetically engineered microbial cells that can produce bacteriocins to control the growth of microbial cells. In some embodiments, microbial cells are contained within a desired environment. In some embodiments, contaminating microbial cells are neutralized. In some embodiments, a first microbial cell type regulates the growth of a second microbial cell type so as to maintain a desired ratio of the two cell types.

The invention discloses a lactobacillus paracasei bacteriocin for inhibiting multi-drug-resistant pseudomonas aeruginosa and a preparation method of the lactobacillus paracasei bacteriocin, and belongs to the field of fermentation engineering. According to the invention, a multi-drug-resistant pseudomonas aeruginosa inhibition zone and lactic acid bacteria growth and acid production are taken as indexes, the casei paracasei KD10 capable of remarkably inhibiting multi-drug-resistant pseudomonas aeruginosa is screened and separated, fermentation conditions are optimized, bacteriocin is obtained by separation and purification from supernate of fermentation liquor, and the amino acid sequence of the bacteriocin is identified. Bacteriostatic experiments show that the lactobacillus paracasei KD10 bacteriocin has broad-spectrum bacteriostasis, and has a certain bacteriostatic effect on various clinically common gram-positive bacteria and gram-negative bacteria. In addition, bacteriocin has good stability to temperature, pH, non-digestive enzymes, storage temperature and ultraviolet rays, and is sensitive to digestive enzymes. The invention provides theoretical and technical references for the development of bacteriocin required for treating and preventing multi-drug-resistant pseudomonas aeruginosa infection.

Described herein are bacteriocin peptides and peptidomimetics as well as compositions comprising same. Aspects and embodiments described herein may be used in medical treatment and disinfection of surfaces.

The invention relates to the technical field of microorganisms, in particular to Weissella fusiformis JWC120 with a function of inhibiting common harmful bacteria in animal breeding and application of the Weissella fusiformis JWC120. The Weissella fusiformis JWC120 with the function of inhibiting common harmful bacteria in animal breeding, provided by the invention, has the preservation number of CGMCC No.31945, and can effectively inhibit common escherichia coli, salmonella, staphylococcus aureus and shigella in animal breeding; a bacteriocin crude extract of the strain has a remarkable inhibition effect on common staphylococcus aureus in animal breeding, and has a good application prospect in animal disease prevention and treatment and development of livestock and poultry breeding health-care products.

In some embodiments herein, methods and compositions for preparing bacteriocins are described. In some embodiments, the propolypeptide can be produced by a microbial cell comprising a nucleic acid encoding the propolypeptide, which comprises a bacteriocin separated by a cleavage site and cis of a desired ratio. In some embodiments, microfluidic devices and methods for preparing a specified mixture of antimicrobial peptides and / or bacteriocins are described.

The present disclosure relates to pharmaceutical compositions comprising live bacteria strains, and / or bacteriocin molecules which can inhibit the growth and persistence of a pathogen such as S. pneumoniae. Further aspects of the present disclosure relate to the use of the pharmaceutical populations for preventing or treating disease caused by S. pneumoniae, or use of compositions comprising live bacteria as probiotics.

The invention discloses a lactobacillus plantarum LF001, the preservation number of the lactobacillus plantarum LF001 is GDMCC No.67171, and the lactobacillus plantarum LF001 integrates the functions of a high-efficiency fermentation agent and a high-efficiency biological preservative into a whole. According to the method, conventional lactic acid fermentation can be completed, the novel performance of high-yield antibacterial peptide can be achieved, broad-spectrum pathogenic bacteria and putrefying bacteria can be actively and effectively inhibited, the preservative and fresh-keeping effect which can only be achieved by common lactic acid bacteria and chemical preservatives / exogenous bacteriocin in the past can be achieved by using a single LF001 strain, the process is simplified, the cost is reduced, and the method is suitable for industrial production. And real'no chemical addition 'is realized.

The present invention belongs to the field of microbial technology, and discloses a strain of Lactobacillus brevis and its antibacterial optimization culture method and application. The strain is Lactobacillus brevis (Lactobacillus brevis) L1612, which has been deposited in Guangdong Provincial Microbial Culture Collection Center on August 4, 2022, with a deposit number of GDMCC 62642. The strain of the present invention has broad-spectrum, efficient antibacterial properties, has the potential to produce new bacteriocins, and is safe and non-resistant. It can be used in the preparation of intestinal antibacterial drugs and in functional foods containing lactic acid bacteria. The present invention also provides an antibacterial optimization culture method for the bacteria, which uses MRS culture medium as a basal culture medium, optimizes the composition ratio and culture conditions in the basal culture medium, and makes the diameter of the inhibition zone of the bacterial fermentation broth increase significantly, which is 25.21% higher than before optimization, thereby facilitating the bacteria to be widely used in the fields of antibacterial drugs and functional food production.

The application discloses a composite biological deodorant, which comprises the following components in parts by weight: 1-3 parts of Bacillus coagulans slurry; 1-3 parts of Saccharomyces cerevisiae slurry; 1-3 parts of Lactobacillus plantarum slurry; 10-30 parts of nutrient solution A; and 20-60 parts of nutrient solution B. The composite biological deodorant is prepared by using the composite system of the Bacillus coagulans slurry, the Saccharomyces cerevisiae slurry and the Lactobacillus plantarum slurry, and combining with a specific nutrient solution carrier. The Bacillus coagulans provides environmental tolerance guarantee for the composite system due to strong environmental resistance. Metabolic products secreted by the Saccharomyces cerevisiae help to promote the proliferation of the Lactobacillus plantarum. Bacteriocins produced by the Lactobacillus plantarum selectively inhibit staphylococcus aureus, escherichia coli and other foul-smelling pathogenic bacteria, and degrade small-molecule foul-smelling substances such as ammonia nitrogen and hydrogen sulfide. The three components are synergistic, so that the deodorization and bacteriostasis effects are achieved. The product has no strong peculiar smell, and the risk of contamination by miscellaneous bacteria is effectively avoided.

This application provides a *Lactobacillus curvatureii* bacteriocin, its preparation method, and its application, relating to the field of biotechnology. The *Lactobacillus curvatureii* bacteriocin provided in this application is obtained through gene recombination expression and is a natural antibacterial ingredient with a broader antibacterial spectrum, low irritation, and long-lasting effect. Applying this *Lactobacillus curvatureii* bacteriocin to feminine hygiene products not only enhances the product's antibacterial effect but also helps repair and maintain the balance of the microecology, preventing disruption of normal flora, effectively maintaining the acidic environment of the intimate area, inhibiting the growth of pathogenic bacteria, and reducing the risk of infection. The feminine hygiene product of this application combines gentleness, high efficacy, and multifunctionality, while also focusing on microecological balance and mucosal repair to meet the higher needs of women's intimate care.

Embodiments herein relate to methods, systems and kits for engineering antimicrobial peptides such as bacteriocins, for example to have a desired range of activity in a desired range of culture conditions. The antimicrobial peptides may be engineered to have a particular activity for a particular culture, environmental conditions or a range of conditions. Some embodiments include screening an antimicrobial peptides or several candidate antimicrobial peptides for a desired activity. Some embodiments include an iterative process for engineering antimicrobial peptides such as bacteriocins. In some embodiments, the process is performed by automated machine learning.

The present invention discloses an enteric Weizmannella coagulans strain RS804 and its application. The present invention provides an enteric Weizmannella coagulans strain RS804 that can efficiently utilize sugar beet sucrose. The strain has certain heat and acid resistance, good polysaccharide hydrolase activities such as cellulase, xylanase and amylase, and can produce L-lactic acid by saccharification and fermentation. In addition, the enteric Weizmannella coagulans strain RS804 can also metabolize and produce a certain amount of bacteriocin, which can effectively inhibit strains with certain competitiveness such as Priesteria gigantea and Bacillus subtilis, and help prevent and control fermentation contamination. The enteric Weizmannella coagulans strain RS804 disclosed in the present invention is of great significance for reducing the production cost of L-lactic acid fermentation and increasing the yield of L-lactic acid.

The present invention belongs to the field of biotechnology and provides a strain of Enterococcus faecalis with a preservation number of CGMCC No. 29087, which is characterized in that the Enterococcus faecalis can secrete bacteriocins that inhibit Vibrio parahaemolyticus, Staphylococcus aureus and Escherichia coli. The present invention also provides the application of the Enterococcus faecalis and the same in aquatic fermented feed and laying hen premix, which can effectively improve the utilization rate of feed and the growth and production performance of animals.

The invention relates to the field of microbiology, in particular to lactobacillus plantarum A3 and application thereof. The lactobacillus plantarum is named as lactobacillus plantarum A3, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 2026320, and the preservation unit is China Center for Type Culture Collection. According to the invention, a safe lactobacillus plantarum A3 is successfully separated and obtained, and a novel bacteriocin Plantaricin A3 is identified from the safe lactobacillus plantarum A3; the bacteriocin Plantaricin A3 has excellent thermal stability, pH stability and protease hydrolysis resistance, is wide in antibacterial spectrum, particularly has a remarkable effect on aquatic pathogenic vibrio, and has a wide application prospect in the fields of food, feed and aquaculture.

The application discloses a bacteriostatic type of liquor dreg containing Zhangzhou Bacillus GBW-NX and a preparation method and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Zhangzhou Bacillus GBW-NX is CGMCC No. 27402. It is proved through experiments that the Zhangzhou Bacillus GBW-NX has the bacteriostatic effect on inhibiting Escherichia coli, Salmonella and Staphylococcus aureus. The Zhangzhou Bacillus GBW-NX is placed in a solid culture medium containing the liquor dreg to be fermented and enzymolyzed to prepare the bacteriostatic type of liquor dreg. The bacteriostatic substance of the bacteriostatic type of liquor dreg is a bacteriocin. It is also proved that the bacteriostatic type of liquor dreg can improve the intestinal function of ruminants including lactating cows, and can also improve the milk yield of the cows, thereby widening the application range of the Zhangzhou Bacillus GBW-NX, providing a new direction for the feeding of ruminants, and having important significance for the breeding of ruminants.