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Method for preparing bioflocculant and use thereof

A technology of biological flocculants and strains, applied in the field of preparation of biological flocculants, can solve the problems of high production cost, low flocculation activity, long fermentation cycle, etc.

Inactive Publication Date: 2012-12-19
SHANGHAI INST OF PHARMA IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that microorganisms such as bacteria, actinomycetes, molds, and yeasts have the ability to synthesize bioflocculants, but their fermentation production generally suffers from high raw material costs, long fermentation cycles, and low flocculation activity. The combined use of inorganic flocculants can play a better effect. These shortcomings make few related products on the market at home and abroad.
[0004] Therefore, there is an urgent need in this field to overcome the shortcomings of high production cost, low flocculation activity, and certain biological flocculants that need to be used in conjunction with inorganic flocculants to achieve better results in the development of existing bioflocculants, and to provide a new bioflocculant Biological flocculant and its excellent preparation technology

Method used

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  • Method for preparing bioflocculant and use thereof
  • Method for preparing bioflocculant and use thereof
  • Method for preparing bioflocculant and use thereof

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Experimental program
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preparation example Construction

[0035] Preparation method of bioflocculant

[0036] The invention provides a preparation method of a biological flocculant, which comprises culturing the bacterial strain Penicillium funiculosum in a fermentation medium to obtain a fermented liquid; centrifuging the fermented liquid to remove mycelia to obtain a supernatant; the supernatant After mixing with ethanol, let it stand still, centrifuge, and take the precipitate to get the bioflocculant.

[0037] The fermentation medium is a liquid medium with neutral pH, preferably pH6.0-8.0; more preferably pH6.5-7.5. It contains nutrients necessary for the growth and metabolism of Penicillium funiculatus, such as but not limited to glucose, sodium nitrate, phosphate and the like.

[0038] The fermentation condition is the culture condition under the shake flask culture state, the pH is neutral, preferably pH6.0-8.0; the inoculum size is 1%, preferably 0.5%-2%; the culture temperature is 25 ℃-30℃, preferably 28℃; the rotation sp...

Embodiment 1

[0055] Preparation of bioflocculant (SIPI-PF)

[0056] 1. Strains

[0057] Penicillium funiculosum (Penicilllium funiculosum) can be purchased from China General Microorganism Culture Collection Center, and the strain preservation number is CGMCC 3.5199

[0058] 2. Fermentation process

[0059] Seed medium (gL -1 ) (purchased from Sinopharm Chemical Reagent Co., Ltd.): sucrose 30, NaNO 3 2, KCl 0.5, K 2 HPO 4 1. MgSO 4 .7H 2 O0.5, FeSO 4 0.01, pH7.2.

[0060] Liquid fermentation medium (gL -1 ) (purchased from Sinopharm Chemical Reagent Co., Ltd.): Glucose 10, NaNO 3 0.5,K 2 HPO 4 2.5, KH 2 PO 4 1. MgSO 4 .7H 2 O 0.2, NaCl 0.1, pH 7.0,

[0061] All the above media were sterilized at 121°C and 0.1MPa for 30min.

[0062] The fermentation conditions are: initial pH 7.0, inoculum size 1%, 28° C., 150 rpm, shaking culture for 60 h.

[0063] 3. Extraction of bioflocculant

[0064] The fermentation broth was centrifuged at 8000rpm for 10min to remove the myc...

Embodiment 2

[0087] Application of bioflocculant

[0088] The cultured fermentation broth was centrifuged to remove mycelium, and the supernatant was retained as flocculant SIPI-PF. Add 90ml of the solution to be flocculated (such as kaolin suspension, bacterial fermentation broth, activated sludge, etc.) into a 100ml graduated cylinder, 5mLw (CaCl 2 )=1% aqueous solution, add SIPI-PF liquid according to different dosage (%, v / v), add distilled water to 100mL, adjust pH to 8.0, pour into 150mL beaker, mix, stir for 3min, let stand for 5min , absorb the supernatant and measure the absorbance (A) at 550nm of the SP-756PC spectrophotometer, and use the absorbance (A) without flocculant 0 ) as a control to determine the flocculation activity of the flocculant, expressed in terms of flocculation rate:

[0089]

[0090] The results are shown in Table 2-4.

[0091] Table 2 The flocculation effect of SIPI-PF on kaolin suspension

[0092] SIPI-PF dosage

(%, v / v)

0.1

...

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Abstract

The invention discloses a method for preparing a bioflocculant and use thereof. The method comprises the following steps of: (a) performing fermentation culture on penicillium funiculosum to obtain fermentation liquor and centrifuging the fermentation liquor to remove mycelium to obtain supernate; and (b), mixing the supernate with ethanol and centrifuging the mixture to obtain the bioflocculant. The bioflocculant can be used for treating activated sludge, fly ash, charcoal, ink, muddy water, river sediment suspension, kaoline suspension, manure water, dyeing and printing wastewater and / or fruit juice.

Description

technical field [0001] The invention relates to a flocculant, in particular to a preparation method and application of a biological flocculant. Background technique [0002] The flocculants widely used in the field of water treatment are mainly inorganic flocculants represented by polyaluminum chloride (PAC) and polymer flocculants represented by polyacrylamide (PAM). These two types of flocculants often cause environmental pollution problems during use, such as Al in drinking water 3+ The increase is the main cause of clinical symptoms of aluminum poisoning, such as aluminum encephalopathy (such as Alzheimer's disease, etc.), aluminum bone disease and aluminum anemia; polyacrylamide monomers have strong neurotoxicity, and there are many Strong carcinogenicity. Therefore, it is imminent to develop efficient, safe, non-toxic and non-secondary pollution flocculants. [0003] Microbial flocculant is a polymer metabolite with flocculation activity produced by microorganisms. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C02F1/54A23L2/82C12R1/80
Inventor 胡海峰王学华朱宝泉
Owner SHANGHAI INST OF PHARMA IND CO LTD
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