33 results about "Penicillium funiculosum" patented technology

The present invention relates to novel micro-organism, Penicillium funiculosum, to new enzymes mixture obtained from it and nucleic acid sequences thereto.

The invention discloses a method for preparing a bioflocculant and use thereof. The method comprises the following steps of: (a) performing fermentation culture on penicillium funiculosum to obtain fermentation liquor and centrifuging the fermentation liquor to remove mycelium to obtain supernate; and (b), mixing the supernate with ethanol and centrifuging the mixture to obtain the bioflocculant. The bioflocculant can be used for treating activated sludge, fly ash, charcoal, ink, muddy water, river sediment suspension, kaoline suspension, manure water, dyeing and printing wastewater and / or fruit juice.

The invention aims at providing an acidic xylanase mutant and an application thereof. Through screening of a plurality of mutants on xylanase of penicillium funiculosum, three mutation sites N104D, T128G and Y129Q can cause improvement of the heat resistance of the xylanase. The optimal reaction temperature of wild xylanase XynH2 provided by the invention is 50 DEG C; the optimal reaction temperatures of mutants XynH2-1 and XynH2-2 are 55 DEG C; the optimal reaction temperature of a mutant XynH2-3 can be 60 DEG C; under the condition of over 53 DEG C, the relative enzyme activity levels of the three mutants are significantly higher than those of the wild mutant; treatment is carried out at 65 DEG C for 5 minutes; the remaining enzymatic activity of the wild xylanase XynH2 only is 5%; and the remaining enzymatic activities of the xylanase mutants XynH2-1, XynH2-2 and XynH2-3 are respectively 20%, 35% and 46%, so that the heat resistance of the screened xylanase mutant is significantly improved.

The invention provides a method for detecting characteristic fungus Amorphotheca resinae in a jet fuel. According to the method, a set of LAMP primers is designed, a detection system with a reaction temperature of 63 DEG C and a time of 35min and directly observed by an LFD test strip is established, and then specificity and sensitivity evaluations are carried out. Results show that the lowest detection lines of detecting A.resinae genome DNA and a pure culture by applying an LAMP-LFD method are 26fg / muL and 8chu / mL respectively; in specificity confirmatory experiments, four fungi namely trichoderma viride, penicillium funiculosum, aspergillus niger and aureobasidium pullulans found in the jet fuel, and A.resinae are used as specificity test strains, and the LAMP-LFD method detection only shows a positive reaction for A.resinae. The detection method disclosed by the invention is capable of rapidly detecting A.resinae in the jet fuel, and visualized in result. The method is short in time consumption, simple and convenient to operate, high in specificity, high in sensitivity and independent of special detection equipment, as well as expected to be used for on-site field detection for characteristic fungus A.resinae in the jet fuel as a new detection method.

The present invention relates to a mutant fungal strain of Penicillium funiculosum ‘MRJ-16’ characterized by the ability to produce high titer of enzyme mixture comprising FPase, CMCase, Cellobiase, β-glucosidase, endoglucanase, α-L arabinofuranosidase, β-xylosidase, xylanase, pectinase, cellobiohydrase and oxidases and produce enzymes in the presence of a catabolite repressor molecule like glucose and / or xylose. The titer of enzyme mixture produced using mutant fungal strain MRJ-16 is at least two fold higher than naive Penicillium funiculosum strain NCIM 1228, when used in a fermentation process. The mutant strain ‘MRJ-16’ with high hydrolytic activity and catabolite derepressed character is having application in the method of degrading or saccharifying biomass to produce valuable products for example-bioethanol.