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Method for producing gluconic acid by intensifying fermentation of glucose via penicillium funiculosum

A technology of Penicillium funicillium and gluconic acid, which is applied in the field of microbial fermentation, can solve the problems of strong inhibition of final products, low fermentation efficiency, and difficulty in increasing the control of culture conditions, so as to improve yield, expand germplasm resources, and realize self-defense Effect

Active Publication Date: 2020-06-26
EZHOU VOCATIONAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above method needs to control the length of Aspergillus niger mycelium, and there are multiple culture conditions to be replaced, which increases the difficulty of controlling the culture conditions.
Moreover, the fermentation process is strongly inhibited by the end product, resulting in low fermentation efficiency
It has been reported that Penicillium genus fungus (Glucose Oxidase Collaborative Group. Penicillium notatum AS3.3871 glucose oxidase research [J]. Microbiology Bulletin (1): 3-7.) high-yield glucose oxidase , but there is no research to apply it to the production of gluconic acid; secondly, it also has the problem of strong inhibition of the end product in the application

Method used

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  • Method for producing gluconic acid by intensifying fermentation of glucose via penicillium funiculosum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for strengthening penicillium fungus to ferment glucose to produce gluconic acid, comprising the following steps:

[0029] S1. Preparation of seed solution: pick the purified Penicillium fungus slant to the beef extract peptone medium, and cultivate it at 30°C and 180rpm for 20h. The bacteria are in the middle of the logarithmic growth phase at this time, with high activity and suitable for fermentation culture. , and then centrifuged to remove the supernatant (seed medium) to prevent the seed medium from interfering with the nutrient content of the fermentation medium, then wash the thalline pellet with sterile saline for 2 to 3 times and then resuspend the thalline to obtain the seed liquid. The concentration of the seed liquid is 1~5×10 9 cfu / mL;

[0030] S2. Fermentation: Inoculate the seed liquid into the fermentation medium according to the inoculation amount of 6%, and cultivate it to the bacterial concentration OD under the conditions of pH 6-8, temper...

Embodiment 2

[0033] Embodiment 2 is basically the same as Embodiment 1, and its difference is:

[0034] S2. Fermentation: Inoculate the seed liquid into the fermentation medium according to the inoculation amount of 6%, and cultivate it to the bacterial concentration OD under the conditions of pH 6-8, temperature 37°C, and rotation speed 230rpm 600nm 2.0 to 2.5, then supplemented with 80g / L glucose and 0.2g / L inducer menadione, and fermented and cultivated for 72 hours under the conditions of pH 6-8, temperature 15°C, and rotation speed 330rpm to obtain gluconic acid.

Embodiment 3

[0036] Embodiment 3 is basically the same as Embodiment 1, and its difference is:

[0037] S2. Fermentation: Inoculate the seed liquid into the fermentation medium according to the inoculation amount of 6%, and cultivate it to the bacterial concentration OD under the conditions of pH 6-8, temperature 37°C, and rotation speed 230rpm 600nm 2.0 to 2.5, then supplemented with 100g / L glucose and 0.2g / L inducer menadione, and fermented and cultivated for 72 hours under the conditions of pH 6-8, temperature 15°C, and rotation speed 330rpm to obtain gluconic acid.

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Abstract

The invention belongs to the technical field of microbial fermentation, and particularly relates to a method for producing gluconic acid by intensifying fermentation of glucose via penicillium funiculosum. The method comprises the following steps: S1, preparation of a seed solution; and S2, fermentation: inoculating a fermentation culture medium with the seed solution according to an inoculum sizeof 4-8%, carrying out culturing under the conditions that a temperature is 35-40 DEG C and a rotating speed is 200-250 rpm until a bacterial concentration OD<600nm> is 2.0-2.5, supplementing glucosewith a concentration of 80-120 g / L and an inducer with a concentration of 0.05-0.5 g / L, and carrying out fermentation culture for 72-96 hours under the conditions that the temperature is 10-20 DEG C and the rotating speed is 300-350 rpm to obtain gluconic acid. By adding the inducer capable of causing oxidative stress damage in the fermentation process, conversion of glucose into gluconic acid ispromoted to consume oxygen free radicals, so oxidative stress damage is relieved, self-defense is achieved, the negative feedback effect of a product is weakened, and finally, the yield of gluconic acid is increased.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for strengthening Penicillium fungus to ferment glucose to produce gluconic acid. Background technique [0002] Gluconic acid can be produced by oxidizing the 1-position aldehyde group of glucose to carboxyl group. It is a colorless to light yellow slurry liquid, easily soluble in water and slightly soluble in alcohol. Usually, a 50% gluconic acid solution is used. Gluconic acid is an important intermediate in chemical, pharmaceutical and food products. It can be used to produce derivatives of gluconic acid, and can also be used directly as a product in the dairy industry to prevent milk stone precipitation. It is used in food formula as a Sour agent, also used to prepare household or factory cleaning agents, textile processing and metal processing additives, leather tanning agents, algae removers, metal rust removers, plasticizers for concrete in the cons...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12R1/80
CPCC12P19/02Y02P20/584
Inventor 周先云杨家林潘朝旺
Owner EZHOU VOCATIONAL UNIV
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