Method for extracting giantarum polysaccharides

A konjac polysaccharide and extraction method technology, which is applied in the field of konjac polysaccharide extraction, can solve the problems of low purity of finished products, many impurities, high price, etc., and achieve the effects of simple and fast extraction process, high product yield and simple equipment

Inactive Publication Date: 2010-12-15
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is a technology that uses cyclone separation after crushing to remove impurities, and sieves to obtain gray and white fine granular products. This method only removes part of the starch and alkaloids, and the product has low purity and many impurities.
Chinese Patent Application No. 200910090261.6 discloses "a method for preparing konjac oligosaccharides", which uses low-concentration konjac powder as a medium to prepare seed liquid or prepare β-glucomannan crude enzyme liquid, direct fermentation or indirectly use crude enzyme Hydrolysis of high-concen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Wash the purchased konjac, take 20kg and freeze it at -30°C, slice the frozen konjac, add 20L of water to make a slurry, centrifuge at a speed of 4000r / min, concentrate the supernatant, add amylase to remove starch (starch The dosage of the enzyme is based on the fact that adding iodine to the concentrated solution does not change the blue color), press filtration, add ethanol until the alcohol concentration reaches 80%, let it stand for precipitation, dissolve the precipitate with 10% ethanol, precipitate with alcohol twice, and freeze the precipitate After drying, 12.6 kg of the product is obtained, and the konjac polysaccharide content is 78%.

Embodiment 2

[0012] Wash the purchased konjac, take 50kg and freeze it at -40°C, slice the frozen konjac, add 100L of water to make a slurry, centrifuge at a speed of 4000r / min, concentrate the supernatant, and add amylase to remove starch (starch The dosage of the enzyme is based on the fact that adding iodine to the concentrated solution does not change the blue color), press filtration, add ethanol until the alcohol concentration reaches 85%, let it stand for precipitation, and then dissolve the precipitate with 10% ethanol, precipitate with alcohol once, and freeze the precipitate After drying, 30kg of the product is obtained, and the konjac polysaccharide content is 82%.

Embodiment 3

[0014] Wash the purchased konjac, take 100kg and freeze it at -30°C, slice the frozen konjac, add 250L of water to make a slurry, centrifuge at a speed of 4000r / min, concentrate the supernatant, add amylase to remove starch (starch The amount of enzyme is based on the fact that adding iodine to the concentrated solution does not change the blue color), press filtration, add ethanol until the alcohol concentration reaches 80%, let it stand for precipitation, and then dissolve the precipitate with 15% ethanol, precipitate with alcohol twice, and freeze the precipitate After drying, 66 kg of product is obtained, and the konjac polysaccharide content is 73%.

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Abstract

The invention relates to a method for extracting giantarum polysaccharides, which comprises the following steps of: cleaning giantarum used as raw material, and then freezing; slicing the frozen giantarum, adding a right amount of water for pulping, centrifuging, and concentrating the supernate; adding diastase to remove starch, filter-pressing, and adding absolute alcohol till the alcohol concentration reaches 80-85%; dissolving the precipitate with water, precipitating with alcohol 1-3 times, and freezing and drying the precipitate to obtain the giantarum polysaccharides. The extraction method is simple, rapid, safe and free of pollution and has high yield and low cost. The content of giantarum polysaccharides in the product is not less than 70%.

Description

Technical field: [0001] The invention belongs to the technical field of natural medicine extraction, and relates to a konjac polysaccharide extraction method. Background technique: [0002] Konjac, also known as konjac, konjac, and ghost head, is the tuber of konjac, konjac, wild konjac, and Dongchuan konjac. "Dictionary of Traditional Chinese Medicine": "Resolving phlegm and eliminating stagnation, detoxifying and dispelling stagnation, promoting blood stasis and relieving pain. Indications for phlegm cough, stagnation, malaria, scrofula, lumps, bruises, carbuncle, furuncle, erysipelas, burns, Snake bite." Konjac contains polysaccharides (such as glucomannan, mannan), organic acids (such as citric acid, ferulic acid, cinnamic acid, etc.). Pharmacological studies have shown that 10% konjac fine powder feed can inhibit the incidence of intestinal cancer and colon cancer in rats induced by 1,2-dimethylhydrazine, and the fine powder can reduce the incidence of spontaneous live...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12S3/02
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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