Preparation method of enteromorpha mushroom polysaccharide compound
The technology of lentinan and prolifera polysaccharide is applied in the field of health care medicines containing the prolifera lentinan polysaccharide compound, and achieves the effects of easy-to-obtain raw materials and reliable preparation process.
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Embodiment 1
[0065] Embodiment 1: Preparation of Enteromorpha polysaccharide
[0066] Enteromorpha samples were washed, air-dried, and chopped. Take 500g of Enteromorpha material, add 7.5L of distilled water, extract at 90°C for 2 hours, filter, and repeat the extraction of the filter residue three times. Combine the filtrates, concentrate to 500mL, add 3 times the volume of 95% ethanol, let it stand overnight at 4°C, and centrifuge (3000r / min) to collect the precipitate to obtain the crude polysaccharide of Enteromorpha enteromorpha. Freeze 2% crude polysaccharide solution of Enteromorpha enteromorpha overnight at -20°C, slowly thaw at room temperature, and centrifuge at a high speed (7000r / min) to remove the precipitate.
[0067] Make a 5% solution of the frozen-thawed Enteromorpha polysaccharide, add ethanol to make the final concentration reach 50%, stay overnight at 4°C, centrifuge (3000r / min) to remove the precipitate, continue to add ethanol to the supernatant to make the concentrat...
Embodiment 2
[0068] Embodiment 2: Preparation of Enteromorpha polysaccharide
[0069] Enteromorpha samples were washed, air-dried, and chopped. Take 5000g of Enteromorpha material, add 75L of distilled water, extract at 90°C for 2 hours, filter, and repeat the extraction three times from the filter residue. Combine the filtrates, concentrate to 1.5L, add 3 times the volume of 95% ethanol, let stand overnight at 4°C, and centrifuge (3000r / min) to collect the precipitate to obtain the crude polysaccharide of Enteromorpha enteromorpha. Freeze 2% crude polysaccharide solution of Enteromorpha enteromorpha overnight at -20°C, slowly thaw at room temperature, and centrifuge at a high speed (7000r / min) to remove the precipitate.
[0070] Make a 5% solution of the frozen-thawed Enteromorpha polysaccharide, add ethanol to make the final concentration reach 50%, stay overnight at 4°C, centrifuge (3000r / min) to remove the precipitate, continue to add ethanol to the supernatant to make the concentratio...
Embodiment 3
[0071] Embodiment 3: preparation of lentinan
[0072] After washing the fruiting bodies of shiitake mushrooms, air-dry and crush them, take 200g shiitake mushrooms and add 4L distilled water, extract at 95°C for 2 h, filter, and repeat the extraction three times from the filter residue. Combine the filtrates, concentrate to 500mL, add 3 times the volume of 95% ethanol, let stand overnight at 4°C, and centrifuge (3000r / min) to collect the precipitated mushroom crude polysaccharide. Prepare 10% Lentinus edodes crude polysaccharide solution, add ethanol to make the final concentration reach 30%, stay overnight at 4°C, centrifuge (3000r / min) to remove the precipitate, continue to add ethanol to the supernatant to make the concentration reach 70%, spend overnight at 4°C, centrifuge (3000r / min) take the precipitate and freeze-dry to obtain lentinan 20.8g.
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