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A molecular marker that co-segregates with aroma traits

A molecular marker and co-separation technology, applied in the field of molecular genetics, can solve the problems such as the unreported rice aroma gene, and achieve the effects of speeding up assisted breeding, saving costs and stabilizing markers.

Inactive Publication Date: 2011-12-07
SHANDONG RICE RES INST
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AI Technical Summary

Problems solved by technology

[0007] The technology of molecular marker-assisted selection of rice aroma genes using molecular markers has been reported at home and abroad, but so far, there has been no report on the co-segregation markers of rice aroma genes, and the use of TPS solution to identify aroma traits is also the first report.

Method used

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  • A molecular marker that co-segregates with aroma traits
  • A molecular marker that co-segregates with aroma traits
  • A molecular marker that co-segregates with aroma traits

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Experimental program
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Embodiment Construction

[0031] 1. F 2 Population creation and phenotyping

[0032]In 2005, Qufu Fragrant Rice, Mingshui Fragrant Rice, and Xiangjing 9407 were used to cross with IRBB60 respectively. At the end of the year, F 1 The seeds were sent to Sanya, Hainan Island for further generation, and self-bred to produce F 2 seed. 2006 will be F 2 The isolated populations were sowed in the Experimental Farm of Shandong Rice Research Institute, sowed on May 1, transplanted on June 20, and transplanted per plant. Transplanting 20 days to extract F 2 Isolate the individual plant DNA of the population, and use the method for identifying rice aroma of the present invention (TPS method) to identify the aroma traits of the individual rice plant, which can be divided into two phenotypes with aroma and no aroma. Plant 3,000 plants in each isolated population, a total of 9,000 plants .

[0033] 2. F 2 Molecular marker analysis of segregated populations

[0034] (1) Extract F from Qufu fragrant rice / IRBB60...

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Abstract

The invention relates to a molecular marker co-separated with aroma traits, belonging to the field of molecular genetics. At the tillering stage, 1-2 young leaves from the upper part of a single rice plant were taken and stored in a -80°C refrigerator for later use. Put 2-4cm long rice leaves into a 1.5ml centrifuge tube, put in liquid nitrogen, grind, add 900 μl of TPS solution, and bathe in 75°C water bath for 20 minutes. Open the cover and smell it with your nose to accurately determine the presence or absence of fragrance. The molecular marker can be obtained by PCR amplification with specific PCR primer L01 or L02. The invention is specially used for the breeding and genetic research of rice aroma varieties, and can be used for cloning aroma genes.

Description

[0001] This application is a divisional application with the original application number 200810015264.9, the filing date is April 18, 2008, and the title of the invention is "a method for identifying the aroma of rice and a molecular marker co-separated with aroma traits". technical field [0002] The invention relates to a molecular marker co-separated with aroma traits, belongs to the field of molecular genetics, is specially used for breeding and genetic research of rice aroma varieties, and can be used for cloning aroma genes. Background technique [0003] Aroma is an important eating quality trait of rice. Many excellent rice varieties have a pleasant aroma and are very popular with consumers. Every rice-producing country or region in the world has fragrant rice planted. [0004] At present, there are mainly methods for the determination of aroma, such as chewing grain method, potassium hydroxide method, and high performance liquid chromatography. The first two methods ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 姚方印李广贤姜明松李润芳张晓东王文英
Owner SHANDONG RICE RES INST
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