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TT16 gene families of brassica napus as well as application thereof

A technology of Brassica napus and BTT16I, which is applied in the field of genetic engineering and achieves the effect of good application prospect

Inactive Publication Date: 2013-07-24
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few research reports on TT16 gene, but the number of members, protein characteristics, evolutionary relationship, tissue specificity of expression and relationship with yellow seed traits of TT16 gene in Brassica species such as Brassica napus, Chinese cabbage, and Brassica oleracea, etc. None reported

Method used

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  • TT16 gene families of brassica napus as well as application thereof
  • TT16 gene families of brassica napus as well as application thereof
  • TT16 gene families of brassica napus as well as application thereof

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Embodiment Construction

[0039] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0040] The plant materials used in the preferred embodiment: the cabbage materials are all from the cabbage-type rape subspecies (B.rapa ssp.oleifera), including the black seed line 09L597 and the yellow seed line 09L600; the cabbage materials are all from the kale variety (B.oleracea var.acephala), including black seed line 09L598 and yellow seed line 09L599; Brassica napus materials include black seed line 5B and seed color near-isogenic lines (black seed line 09L588, yellow seed line 09L587), a...

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Abstract

The invention discloses TT16 gene families of brassica napus and parental species celery cabbage and cabbage of the same as well as application thereof, wherein the TT16 gene family of the celery cabbage comprises a BrTT16-1 gene, a BrTT16-2 gene and a BrTT16-3 gene; the TT16 gene family of the cabbage comprises a BoTT16-1 gene, a BoTT16-2 gene and a BoTT16-3 gene; and the TT16 gene family of thebrassica napus comprises a BnTT16-1 gene, a BnTT16-2 gene, a BnTT16-3 gene, a BnTT16-4 gene, a BnTT16-5 gene and a BnTT16-6 gene. The TT16 gene families can be used for the molecular breeding of seedcharacters of brassica crops.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to Brassica napus and its parent species Chinese cabbage (Brassica rapa) and cabbage (Brassica oleracea) TT16 (TRANSPARENT TESTA 16, transparent seed coat 16; also known as ABS, ARABIDOPSIS BSISTER ; or AGL32, AGAMOUS-LIKE 32) gene family and applications thereof. Background technique [0002] The genus Brassica (Brassica) of the Brassicaceae (Brassicaceae) includes many oil crops, vegetables and ornamental plant species. It provides humans with nutritious edible oils, vegetables and ornamental plants, and provides feed for animal husbandry. It has an important economic value. Among the Brassica species, the allotetraploid species Brassica napus was formed from two diploid species Brassica oleracea and Brassica oleracea through interspecific hybridization followed by remultiplication. Brassica napus is the second largest oil crop in the world, widely planted all over ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C12N5/10A01H5/00
Inventor 柴友荣马丽娟闫楠雷波谌利吕俊李加纳马赑周清元
Owner SOUTHWEST UNIV