Acetone-butanol in-situ extraction continuous fermentation device and technology

A technology of acetone butanol and fermentation process, applied in fermentation, biochemical cleaning device, enzymology/microbiology device and other directions, can solve the problems of difficulty in large-scale production, high equipment price, poor stability, etc., and achieves easy implementation, The effect of increasing yield and reducing dosage

Inactive Publication Date: 2011-01-19
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, due to the poor stability and high equipment prices of these technologies in

Method used

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  • Acetone-butanol in-situ extraction continuous fermentation device and technology
  • Acetone-butanol in-situ extraction continuous fermentation device and technology
  • Acetone-butanol in-situ extraction continuous fermentation device and technology

Examples

Experimental program
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Effect test

Example Embodiment

[0023] Example 1:

[0024] 1) Using corn steep liquor as raw material, its content in the culture medium is 20g / L, and additional nutrients are 0.3g / LCaCl 2 , 0.2g / L MgSO 4 ·7H 2 O, 0.5g / L KH 2 PO4, 0.5g / L urea; equipped with 0.5M NaOH injection lye tank; oleyl alcohol stock solution injected into the extractant storage tank for later use. The fermentation begins to steam sterilize the equipment in situ.

[0025] 2) Preparation of seed liquid: Clostridium acetobutylicun (CGMCCAS1.132) is used as the fermentation strain (purchased from the Common Microorganism Collection Center of the Chinese Microorganism Collection Management Committee), the composition of each liter of seed culture medium is: 40g glucose , 6g tryptone, 2g yeast extract, 2g beef extract, 3g (NH 4 ) 2 SO 4 , 0.2g MgSO 4 ·7H 2 O, 0.5g KH 2 PO 4 , 0.01g FeSO 4 ·7H 2 O, adjust pH=6.0~6.5. Inject the seed culture medium into the No. 1 seed fermentation tank, the injection volume is 2 / 3 of the volume of the fermentatio...

Example Embodiment

[0032] Example 2:

[0033] 1) Using cassava pulp as raw material, the content in the medium is 40g / L, and the additional nutrients are 0.3g / LCaCl 2 , 0.2g / L MgSO 4 ·7H 2 O, 0.5g / L KH 2 PO4, 0.5g / L urea; equipped with 0.5M NaOH injection lye tank; oleyl alcohol stock solution injected into the extractant storage tank for later use. The fermentation begins to steam sterilize the equipment in situ.

[0034] 2) Preparation of seed liquid: Clostridium acetobutylicunm (CGMCCAS1.244) was used as the fermentation strain (purchased from the General Microorganism Collection Center of the China Microorganism Collection Management Committee).

[0035] The steps are the same as in Example 1, and the culture temperature is set to 35°C.

[0036] 3) After 7 hours of seed solution cultivation, turn on the raw material circulation pump of the first-level fermentation tank to make the raw materials enter 2 / 3 of the volume of the fermentation tank, turn off the pump, and connect 8% of the seeds in the fi...

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Abstract

The invention provides an acetone-butanol in-situ extraction continuous fermentation device and technology. The device comprises an No.1 seeding tank and an No.2 seeding tank which are alternately inoculated. Acetone-butanol fermentation is divided into two stages to continuously operate, wherein the first stage is conventional acid production period fermentation, and the second stage is in-situ extraction product synthesis period fermentation; the second period adopts a method of coupling extraction and fermentation and is matched with low-speed stirring to furthest lower product inhibiting effect and greatly improve butanol fermentation efficiency; and finally, static phase processing realizes the initial separation of mash and fermentation products. The invention has the advantages that the device and the technology have good extraction effect in production, can obviously improve butanol ratio in the fermentation product, can greatly lower production cost, and have obvious economic benefit. The technology is scientific and reasonable. The flow device is simple and easy to realize, can realize the new fermentation technology provided by the invention by slightly reforming the traditional butanol fermentation flow, and has wide application prospect.

Description

technical field [0001] The invention relates to acetone-butanol fermentation technology, in particular to an acetone-butanol in-situ extraction continuous fermentation device and process. Background technique [0002] Butanol is a large-scale power fuel that can replace gasoline, and its physical and chemical properties are closer to gasoline than ethanol. At present, the industrial production methods of butanol mainly include propylene oxo synthesis method, aldol condensation method and fermentation method. The first two mainly use petroleum resources as raw materials, but with the increasing depletion of petroleum resources, this method is no longer suitable for large-scale industrial production. The production of butanol by fermentation method is mainly produced by anaerobic fermentation of Clostridium acetobutylicum, using corn (corn, corncob, rye, wheat) starch as raw material, adding water to mix into mash, cooking and sterilizing, adding pure acetone butanol Clostri...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/02C12P7/16C12R1/145
Inventor 应明
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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