Human transient receptor potential channel protein fluorescence quantitative PCR detection kit and preparation method and application thereof

A technology of transient receptor potential and detection kits, applied in biochemical equipment and methods, fluorescence/phosphorescence, microbial measurement/inspection, etc., can solve the problems of low operability, time-consuming, cumbersome process, etc., and achieve operability Strong, good operability and repeatability, easy to save the effect

Inactive Publication Date: 2011-01-19
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT) +1
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  • Human transient receptor potential channel protein fluorescence quantitative PCR detection kit and preparation method and application thereof
  • Human transient receptor potential channel protein fluorescence quantitative PCR detection kit and preparation method and application thereof
  • Human transient receptor potential channel protein fluorescence quantitative PCR detection kit and preparation method and application thereof

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[0127] The following describes the kit in further detail with application examples:

[0088] Reference Figure 1a , Figure 1b , Figure 1c with Figure 1d This embodiment includes a box body, in which a reaction plate 1 and a positive control substance 2 are arranged, and six groups of reaction wells 3 are arranged in the reaction plate 1, and the reaction plate 1 includes a fluorescent quantitative PCR standard curve reaction plate 11 and a fluorescent quantitative PCR test sample reaction plate 12; the target gene TRPC1~TRPC7 reaction mixture and internal reference gene IP08 reaction mixture are set in the fluorescence quantitative PCR standard curve reaction plate 11 and the fluorescence quantitative PCR sample reaction plate 12, the target gene TRPC1~ TRPC7 reaction mixture includes TRPC1~TRPC7 gene primer sequence, SYBR fluorescent dye, DNA polymerase and reaction ion buffer, internal reference gene IP08 reaction mixture includes IP08 gene primer sequence, SYBR fluorescent dy...

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Abstract

The invention relates to a human transient receptor potential channel (TRPC) protein fluorescence quantitative PCR detection kit and a preparation method and application thereof. The detection kit comprises a box body, wherein reaction plates (1) and a positive reference substance (2) are arranged in the box body. The detection kit is characterized in that a plurality of reaction holes are arranged in the reaction plates (1); the reaction plates (1) contain a fluorescence quantitative PCR standard curve reaction plate (11) and a fluorescence quantitative PCR sample under test reaction plate (12); the target gene TRPC1-TRPC7 reaction mixed liquid and the reference gene IP08 reaction mixed liquid are placed in the fluorescence quantitative PCR standard curve reaction plate (11) and the fluorescence quantitative PCR sample under test reaction plate (12); and the target gene TRPC1-TRPC7 reaction mixed liquid and the reference gene IP08 reaction mixed liquid are placed in the reaction holes. The detection kit of the invention is characterized by convenient preparation and storage and good operability and repeatability, various detection kits can be designed according to different TRPC gene research emphases, and the TRPC relatively quantitative analysis of a plurality of samples can be completed at once.

Description

technical field [0001] The invention relates to a human transient receptor potential channel protein fluorescent quantitative PCR detection kit and a preparation method and application thereof. Belongs to the field of biotechnology. Background technique [0002] Transient receptor potential channel (abbreviation: TRPC) is a non-selective cation channel family (including TRPC1~TRPC7), which is permeable to calcium ions. This family protein has six highly conserved transmembrane regions. According to the structure and function The similarities can be divided into 4 subgroups: TRPC1; TRPC2; TRPC3, TRPC6 and TRPC7; TRPC4 and TRPC5. TRPC can form a functional SOCC channel through homologous or heterotetramer combination, which mediates the generation of SOCE and causes cellular calcium influx. Abnormal expression of TRPC can cause changes in SOCE to affect cell life activities and participate in various benign and malignant diseases. For example: respiratory system diseases: as...

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 卢文菊王健何建行张奇张雅洁张晨婷
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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