High-efficiency binding peptide in DNA binding region protein of FoxM1c and method for acquiring polypeptide structure sequence

A technology of structural sequence, foxm1c, applied in the field of biotechnology pharmacy

Inactive Publication Date: 2011-02-16
SOUTHWEST JIAOTONG UNIV
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  • High-efficiency binding peptide in DNA binding region protein of FoxM1c and method for acquiring polypeptide structure sequence
  • High-efficiency binding peptide in DNA binding region protein of FoxM1c and method for acquiring polypeptide structure sequence
  • High-efficiency binding peptide in DNA binding region protein of FoxM1c and method for acquiring polypeptide structure sequence

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[0018] : 298-304); 6) Confirm the high affinity and specificity of the screened polypeptide for FoM1 by methods such as affinity reverse screening; 7) The binding peptide and the structural sequence of the polypeptide contained in it are used as lead molecules for tumors, etc. Drug development and diagnosis of related diseases and other uses: A: Like the current peptide drug, it is used for the treatment of tumors and other diseases; B: Like the existing commercial HA, 6×histidine and other molecular labeling methods, it is constructed In the protein expression vector, it is used for molecular detection of protein expression by immunodetection methods such as Western.

[0019] The present invention will be further described below in conjunction with the examples, but it does not represent the only embodiment of the present invention. In order to realize the above object, the following technical steps of the present invention. 1) Construction of prokaryotic recombination-induc...

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Abstract

The invention discloses a high-efficiency binding peptide in a DNA binding region protein of FoxM1c, a method for acquiring a polypeptide structure sequence and application of a target product. In the invention, protein obtained by inducing and expressing with a pronucleus recombinant plasmid in the DNA binding region of the FoxM1c is used as a target, and a set of targeted FoxM1c high-efficiency binding polypeptides are screened and obtained from a phage random peptide library. The method comprises the following steps of: constructing the pronucleus recombinant plasmid in the DNA binding region of the FoxM1c, carrying out induction expression and protein purified preparation; and screening and analyzing the phage random peptide library on the basis of the DNA binding region protein of the FoxM1c; and screening for four rounds to obtain the binding peptide phage and the polypeptide structure sequence of the enriched and targeted FoxM1c. The target product used as a precursor molecule is used for developing medicaments and diagnosing for treating related diseases of tutors, and the like.

Description

technical field [0001] The invention relates to the field of biotechnology and pharmacy. In particular, the preparation of FoxM1c's DNA binding region protein, the confirmation of the highly efficient binding peptide structural sequence, and its potential application as a lead molecule in the development and diagnosis of drugs for the treatment of tumors and other related diseases. [0002] Background technique [0003] Malignant tumors are major diseases that seriously endanger human life and health. Among the various transcription factors associated with tumors, there are many Fox family proteins. According to the homology of the DNA binding region, more than 100 Fox family members have been confirmed in different species, belonging to 17 subfamilies. Fox family proteins have a wide range of biological functions, involving various biological processes such as embryonic development, cell cycle regulation, sugar and lipid metabolism, aging, and immune regulation. Their mu...

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Application Information

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IPC IPC(8): A61P35/00A61K38/07A61K38/06A61K38/08C07K1/00C12N15/70C12N15/12C07K14/47C07K1/22
Inventor 茆灿泉崔健郭泰林
Owner SOUTHWEST JIAOTONG UNIV
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