Unlock instant, AI-driven research and patent intelligence for your innovation.
avrxa23 protein activating disease-resistant reaction of rice and coding gene thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A coding gene, rice bacterial blight technology, applied in the field of non-toxic genes interacting with rice host disease resistance genes, can solve the problem that no natural strains have been found
Active Publication Date: 2013-06-26
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
For more than 20 strains from Vietnam, South Korea, Bangladesh and other countries that can cause Xa4 and Xa21 to cause disease, Xa23 is highly resistant, and no natural strains that can overcome Xa23 resistance have been found so far
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0042] Embodiment 1, the discovery of AvrXa23 gene
[0043] 1. Xanthomonas oryzae PXO99 A Construction of the mutant library
[0044] Using the Tn5 transposition system [EZ-Tn5 TM TnpTransposome TM Kit (Epicentre Technologies, Madison, WI)] Build PXO99 A mutant library.
[0045] 1. Competent cell preparation
[0046] PXO99 A Bacteria were streaked on XBZ medium (XBZ) and cultured at 28°C for activation. Pick a single clone colony and plate it on XBZ solid medium, and culture it for 2 days. Take a small amount of bacterial cells with an inoculation loop, inoculate them into liquid medium, and cultivate them with shaking at 28°C and 220rpm. When OD 600 When = 0.6, stop shaking immediately, take it out, put it in ice water and shake it quickly until it is completely cooled, then divide it into 50ml centrifuge tubes, and centrifuge at 2200g (g=rcf) at 4°C. Pour off the supernatant, add 30ml of 10% sterilized glycerol, and shake in ice water until the precipitat...
Embodiment 2
[0081] Example 2, Verification of functional complementation of AvrXa23 gene
[0082] 1. Preparation of recombinant bacteria XM81 / L-2
[0083] 1. Competent cell preparation
[0084] Streak the mutant strain XM81 on the XBZ solid medium and culture it at 28°C for activation. Pick a single clone colony and plate it on XBZ solid medium, and culture it for 2 days. Take a small amount of bacterial cells with an inoculation loop, inoculate them into XBZ liquid medium, and cultivate them with shaking at 28°C and 220rpm. When OD 600 When = 0.6, stop shaking immediately, take it out, put it in ice water and shake it quickly until it cools down completely, divide it into 50ml centrifuge tubes, and centrifuge at 2200g, 4°C. Pour off the supernatant, add 30ml 10% sterilized glycerol, shake in ice water until the precipitate is completely dissolved, and centrifuge at 2400g, 4°C. Pour off the supernatant, mix thoroughly when the remaining 0.5ml is left, put 60μl / tube into 1.5m...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses an avrXa23 protein activating disease-resistant reaction of rice and coding gene thereof. The protein provided by the invention is (a) or (b), wherein (a) is protein composed of amino acid sequence shown as sequence 1 in sequence list, (b) is protein formed by the way that substitution and / or deletion and / or addition of one or more than one amino acid residues are carried out on amino acid sequence shown as the sequence 1 and derivation related to activating of disease-resistant reaction of rice is carried out on the sequence 1. Coding gene of avrXa23 protein obtained by the invention can be taken as gene resource of rice bacterial leaf blight resistant disease-resistant breeding, gene is introduced into rice xanthomonas oryzae pv. oryzae XM81 strain, and rice containing Xa23 disease-resistant gene can produce disease resistance, so that xanthomonas oryzae pv. oryzae is difficult to effectively infect rice. The invention is conductive to researching typical specificity interaction between plant and pathogenic bacteria and is conductive to clarifying broad spectrum pathogenicity mechanism of xanthomonas oryzae pv. oryzae.
Description
technical field [0001] The invention belongs to the field of genetic engineering, and relates to avrXa23 protein and its coding gene (pathogen avirulent gene) that stimulate rice to produce disease resistance response, in particular to a disease-resistant gene that is isolated from rice bacterial blight pathogen and is compatible with rice host Interacting avirulent genes. Background technique [0002] Rice is an important food crop in my country, and about 60% of the population takes rice as a staple food. The sustainable development of rice production is directly related to the food security of our country. Rice bacterial blight (Bacterial blight, BB) is one of the main diseases in rice production in the world. It was first discovered in Fukuoka, Japan in 1884. Since the 1950s, the incidence range has been expanding. It has spread all over the rice producing areas of the world. Rice bacterial blight is a vascular disease caused by the gram-negative bacterium Xanthomonas ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More