Preparation method of superoxide dismutase
A technology of superoxide and dismutase, which is applied in the field of superoxide dismutase preparation, can solve the problems of lack of eukaryotic modification steps, etc., and achieve the effects of energy saving, high enzyme expression and high recovery rate
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[0023] (1) Seed culture:
[0024] Prepare seed medium (glucose 30 g, soybean flour 20 g, glycerin 70 g, protein 2 g, NaNO 3 2 g, MgSO 4 1 g, water 1000 mL) 300 mL, placed in a 1000 mL Erlenmeyer flask, at 1.1kg / cm 2 Sterilize under pressure for 30 minutes, then inoculate purple red yeast rice ( Monascus purpureus Went., the strain preservation number is: CGMCC 3.5833) culture suspension of slant. Cultivate for 3-4 days in 28° shake flask at 200 rpm.
[0025] (2) Fermentation of purple red yeast rice:
[0026] The fermentation medium is composed of the following ingredients: 1000 mL of potato extract, 20 g of glucose, and natural pH. Take 200 grams of potatoes, cut them into small pieces, add 1.0 liter of water and boil for 30 minutes, filter out the potato pieces, and make up the filtrate to 1.0 liters.
[0027] Take 3 L of the above-mentioned culture medium and put it into a 5 L fermenter, at 1.1kg / cm 2 Sterilize under pressure for 30 minutes. Inoculate the above c...
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