Preparation method of superoxide dismutase

A technology of superoxide and dismutase, which is applied in the field of superoxide dismutase preparation, can solve the problems of lack of eukaryotic modification steps, etc., and achieve the effects of energy saving, high enzyme expression and high recovery rate

Inactive Publication Date: 2011-02-16
苏州泰利三佳纳米科技有限公司
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, bacterial enzymes lack eukaryotic modification steps after gene expression, so the application of SOD without eukaryotic sources has obvious effects on human health and life in practice

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of superoxide dismutase
  • Preparation method of superoxide dismutase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Seed culture:

[0024] Prepare seed medium (glucose 30 g, soybean flour 20 g, glycerin 70 g, protein 2 g, NaNO 3 2 g, MgSO 4 1 g, water 1000 mL) 300 mL, placed in a 1000 mL Erlenmeyer flask, at 1.1kg / cm 2 Sterilize under pressure for 30 minutes, then inoculate purple red yeast rice ( Monascus purpureus Went., the strain preservation number is: CGMCC 3.5833) culture suspension of slant. Cultivate for 3-4 days in 28° shake flask at 200 rpm.

[0025] (2) Fermentation of purple red yeast rice:

[0026] The fermentation medium is composed of the following ingredients: 1000 mL of potato extract, 20 g of glucose, and natural pH. Take 200 grams of potatoes, cut them into small pieces, add 1.0 liter of water and boil for 30 minutes, filter out the potato pieces, and make up the filtrate to 1.0 liters.

[0027] Take 3 L of the above-mentioned culture medium and put it into a 5 L fermenter, at 1.1kg / cm 2 Sterilize under pressure for 30 minutes. Inoculate the above c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method of metalloenzyme widely existing in organisms, in particular to a preparation method of superoxide dismutase. The method is characterized by comprising seed culture, fermentation of monascus purpureus went., thalli disruption, crude enzyme acquisition and enzyme scouring and is specifically characterized by culturing the thalli, then using high pass oxygen to induce enzyme to express, using a homogenizer to disrupt the thalli, using 70% of ammonium sulfate for precipitation and using SephdexG-25 gel salt, thus obtaining the superoxide dismutase product. Compared with the prior art, the product has high enzyme expression quantity and recovery rate, saves energy and lowers the cost.

Description

technical field [0001] The invention relates to a preparation method of a metalloenzyme widely present in organisms, especially a preparation method of superoxide dismutase. Background technique [0002] Superoxide dismutase (superoxide dismutase, referred to as SOD) is a metalloenzyme widely present in organisms, which can catalyze the dismutation reaction of superoxide anion free radicals and balance oxygen free radicals in the body. Popular research topics in the field of study. As a cleaning agent for superoxide anion free radicals in organisms, SOD has unique functions in radiation protection, anti-aging, anti-inflammation, tumor and cancer suppression, autoimmune therapy, etc. Many applications. Therefore, the demand for SOD is also increasing. [0003] So far, people have isolated SOD from organisms such as bacteria, protozoa, algae, mold, plants, insects, birds, fish, and mammals. Therefore, people in the industry now have a wide range of sources for extracting SO...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08C12R1/645
Inventor 周国富郭锋利许晓东
Owner 苏州泰利三佳纳米科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap