Multifunctional fusion enzyme XABT gene and construction method and application thereof

Abstract

The invention discloses a multifunctional fusion enzyme XABT gene and a construction method and application thereof; the multifunctional fusion enzyme XABT gene can simultaneously express xylanase, phytase, glucanase and cellulase and has high enzyme activity; the multifunctional fusion enzyme enhances glucanase activity and, therefore, allows for a greater ability to digest barley, oat, rye, wheat and their bran rations with higher glucan content. The multifunctional fusion enzyme XABT gene can also be used for producing grain-saving environment-friendly transgenic economic animals; main anti-nutritional factors in feed are eliminated, so that the purposes of increasing feed digestibility and reducing pollution emission are achieved.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a multifunctional fusion enzyme XABT gene and its construction method and application. Background technique

[0002] Feed contains a large amount of cellulose, hemicellulose (arabinoxylan, β-glucan, mannan, etc.), pectin and other non-starch polysaccharide (NSP) anti-nutritional factors, which affect the nitrogen and energy utilization of feed. major antinutritional factor. Phytic acid is the main form of phosphorus in plants. Pigs lack phytase and cannot digest and utilize phosphorus in it, resulting in waste of plant-sourced phosphorus and environmental pollution.

[0003] Although the application of non-starch multienzymes and phytases in the feed industry has achieved initial success, there are still a series of problems in their practical production and application. The enzyme preparation industry mainly relies on microbial fermentation to produce industrial enzymes. There are...

Images