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46 results about "Metallo enzyme" patented technology

Metalloenzymes are enzymes that contain a tightly bound metal ion. This metal ion forms coordinate covalent bonds with the amino acids of the enzyme or with a prosthetic group. Further, it acts as a coenzyme and imparts the activity of the enzyme. When considering the location of the metal ion in the enzyme,...

Heavy metal enzyme label and application thereof

The invention discloses an enzyme label and application thereof. The heavy metal enzyme label is a conjugate consisting of a complex and an enzyme, wherein the complex is a complex consisting of a conjugate of a chelant and a carrier protein and heavy metal. A method for preparing a copper-ion enzyme label has the advantages of simpleness, low synthesis cost and low HRP activity loss. The ELISA detection method constructed by the enzyme label has extremely high sensitivity. The method for preparing the enzyme label for copper ion ELISA detection has important reference value in successfully preparing an enzyme label for ELISA detection of other heavy metals, and the copper-ion rapid immunological detection method constructed by the method has wide application prospect.
Owner:CHINA AGRI UNIV

Methods of generating antibodies to metalloenzymes

A method of generating an antibody which inhibits a metalloenzyme is disclosed. The method comprises immunizing a subject with:(i) a synthetic zinc mimicry compound having structural and electronic properties similar to a catalytic domain of the metalloenzyme; and(ii) the metalloenzyme,Antibodies generated by this method are also disclosed as well as uses thereof.
Owner:YEDA RES & DEV CO LTD

A kind of decolorizing peroxidase and its preparation method and application

The invention belongs to the field of manual metalloenzyme and discloses high-efficiency decolorizing peroxidase and a preparation method and application thereof. According to the decolorizing peroxidase disclosed by the invention, myohemoglobin with an oxygen carrying function is utilized as protein molecules to design a framework, tyrosine is introduced to a 43 site near the heme active center of the myohemoglobin, and tryptophan is introduced to a 138 site; meanwhile, tryptophan is introduced to an 88 site at the periphery of the heme active center to obtain three-point mutant protein, andan amino acid sequence of the three-point mutant protein is shown as SEQ ID NO.1. According to the decolorizing peroxidase disclosed by the invention, a catalytic conversion number is improved, and dependence on hydrogen peroxide is reduced; the preparation method of the decolorizing peroxidase has the advantages of simpleness in operation and suitability for large-scale industrial production. Experiments show that the decolorizing peroxidase disclosed by the invention can degrade dye molecules under low-concentration hydrogen peroxide and within several seconds to achieve a decolorizing effect, a catalytic efficiency is increased by 130% compared with that of two-point mutant protein, and the decolorizing peroxidase can be widely applied to pollution treatment of industrial dye wastewater.
Owner:NANHUA UNIV

Millimeter ball catalyst for one-step synthesis of chiral alcohol and chiral ester from acetophenone

The invention belongs to the field of catalytic materials, and particularly relates to a millimeter ball catalyst for one-step synthesis of chiral alcohol and chiral ester from acetophenone. Metal andenzyme are assembled in the catalyst, the particle size is 0.9-3.0 mm, and the mechanical strength is as high as 3.0-15.0 N. A preparation method comprises the following steps of adding silicon oxidenanoparticles loaded with metal nanometer Pd and silicon oxide nanoparticles loaded with candida antarctica lipase B into deionized water, and performing uniform stirring and mixing to obtain a two-component silicon oxide mixed solution; continuously dripping the double-component silicon oxide mixed solution on tiled super-hydrophobic silicon oxide powder, and performing slow rolling to form a droplet millimeter ball which has a silicon oxide hydrophobic shell; collecting the droplet millimeter ball, and slowly drying the droplet millimeter ball; and finally, performing drip washing with acetone, and performing vacuum drying to obtain the millimeter ball for encapsulating the metal and enzyme dual-catalyst. The catalyst can be directly loaded into a fixed bed reactor to synthesize the chiral alcohol and the chiral ester from the acetophenone in one step.
Owner:SHANXI UNIV
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