49 results about "Metallo enzyme" patented technology

A stable composition preferably a refreshing drink for accelerating degradation of alcohol and acetaldehyde in the body, comprises synergistic combination of caffeine, herbs and fructose. Preferably, the composition comprises, Caffeine, Guaranà caffeine, Yerba Matè, Eleutherococcus senticous, Panax ginseng, ginger, Glycyrrhiza glabra (Liquorice Root), fructose and additional ingredients (sugars, flavourings, colourings, vitamins, stabilisers, whole fruit powder and the like), and optionally ginkgo biloba.

The present invention is a method for identifying a compound as a competitive, noncompetitive, or uncompetitive inhibitor of an enzyme having a functional metal cation, the method comprising assaying the compound for inhibition of the enzyme in the presence of a ligand to the functional metal cation.

There are provided inter alia metalloenzyme inhibitors, such as inhibitors of influenza A RNA dependent RNA polymerase PA subunit endonuclease, and methods of synthesis and use of the same.

The invention discloses an enzyme label and application thereof. The heavy metal enzyme label is a conjugate consisting of a complex and an enzyme, wherein the complex is a complex consisting of a conjugate of a chelant and a carrier protein and heavy metal. A method for preparing a copper-ion enzyme label has the advantages of simpleness, low synthesis cost and low HRP activity loss. The ELISA detection method constructed by the enzyme label has extremely high sensitivity. The method for preparing the enzyme label for copper ion ELISA detection has important reference value in successfully preparing an enzyme label for ELISA detection of other heavy metals, and the copper-ion rapid immunological detection method constructed by the method has wide application prospect.

The invention discloses a method for detecting propyl gallate and formaldehyde, which is characterized in that a zinc-based monatomic nano-enzyme is synthesized by a salt template method, based on the characteristic that Zn-N-C zinc has electron and geometric structures similar to those of the active center of natural metalloenzyme, and the strong simulated peroxidase activity is shown. The substrate propyl gallate can be rapidly oxidized into a yellow oxidized quinones substance, and formaldehyde can inhibit the oxidation reaction, so that the rapid detection method of PG and formaldehyde is established, and the method is applied to detection and analysis of PG and formaldehyde in food. The result of the method is consistent with a method for determining nine antioxidants in food in GB5009.32-2016 food safety national standard and a method for determining formaldehyde in food in GB / T5009.49; the method can only selectively and rapidly oxidize PG, the reaction speed of other antioxidants is low, and the method has the advantages of being high in sensitivity, high in specificity, easy to operate, rapid and the like.

The invention relates to a preparation method of metalloenzyme widely existing in organisms, in particular to a preparation method of superoxide dismutase. The method is characterized by comprising seed culture, fermentation of monascus purpureus went., thalli disruption, crude enzyme acquisition and enzyme scouring and is specifically characterized by culturing the thalli, then using high pass oxygen to induce enzyme to express, using a homogenizer to disrupt the thalli, using 70% of ammonium sulfate for precipitation and using SephdexG-25 gel salt, thus obtaining the superoxide dismutase product. Compared with the prior art, the product has high enzyme expression quantity and recovery rate, saves energy and lowers the cost.

The present invention is drawn to artificial metalloenzymes for use in cyclopropanation reactions, amination and C—H insertion.

The invention provides a hydrogen production nano-device, a production method thereof, and a hydrogen production method thereof. The hydrogen production nano-device comprises metal nano-particles, a photoinduced electron donor, alpha-helical peptides and organic metalloenzyme. The hydrogen production nano-device can use renewable solar energy to realize efficient and pollution-free hydrogen generation, all raw materials used in the invention have very good environment endophilicity and no pollution, and the macroscopic dipole moment characteristic of the alpha-helical peptides is used to improve the unidirectional electron delivery efficiency and improve the hydrogen yield, so the problems of environment pollution and energy consumption of present hydrogen production methods are effectively solved.

The invention belongs to the field of artificial metalloenzyme and discloses an efficient dehalogenated peroxidase and a preparation method and application thereof. The dehalogenated peroxidase utilizes myohemoglobin having an oxygen delivery function as a protein molecule design framework, the site 43 nearby the active center of myohemoglobin protoheme are mutated into tyrosine, meanwhile histidine at the site 64 is mutated into acidic aspartic acid, accordingly double-point mutant protein F43Y / H64DMb having a catalytic function is established, and its amino acid sequence is shown as SEQ ID NO.1. The artificial dehalogenated peroxidase can catalyze and oxidize halophenols into low-toxicity quinone products under the condition of low H2O2 concentration within several seconds, achieves a dehalogenation effect and greatly improves the affinity of the double-mutant protein to the halophenols under the situation that the catalytic conversion number is similar, its catalytic efficiency is obviously improved compared with a single-point mutant protein, and accordingly the dehalogenated peroxidase can be widely applied to treatment of halogenated phenol type organic pollutants.

An organic polyamine 1[bis(3-aminopropyl) amino]-2-propanol, that is, G9H23N3O, its preparing process and its application as chemical intermediate in preparing chelating agent, corrosion inhibitor, resin solidifying agent and catalyst and researching metallic enzyme are disclosed.

A method of generating an antibody which inhibits a metalloenzyme is disclosed. The method comprises immunizing a subject with:(i) a synthetic zinc mimicry compound having structural and electronic properties similar to a catalytic domain of the metalloenzyme; and(ii) the metalloenzyme,Antibodies generated by this method are also disclosed as well as uses thereof.

The invention discloses nucleoside triphosphate artificial metalloenzyme as well as preparation and application thereof. The metalloenzyme has a structural formula shown in the description, in the formula, n represents 1, 2 or 3; A represents any one of formulae shown in the description; B represents H or OH; and C represents any one of Cu(OTf)2, CuSO4, CuCl2, Cu(NO3)2 and formulae shown in the description. The nucleoside triphosphate artificial metalloenzyme is formed by nucleoside triphosphate as a host molecule together with metallic ions or metal complexes through supramolecular self-assembly, the preparation method is simple and convenient, the structure is novel, the metalloenzyme can be adopted to catalyze an asymmetric Diels-Alder reaction and an asymmetric Michael reaction in a water phase, the catalysis activity is high, and high enantio-selectivity can be achieved.

The invention belongs to the field of artificial metalloenzyme, and discloses an efficient azo red dye decolorized enzyme and a preparation method and application thereof. According to the azo red dyedecolorized enzyme, myoglobin having the function of transporting oxygen is used as a protein molecule design framework, tyrosine is introduced to the 43rd site near the active center of myoglobin hemachrome, tryptophan is introduced to the 138th site, arginine is introduced to the 67th site, three-point mutant protein F43Y / F138W / T67R Mb is obtained, and the amino acid sequence is as shown in SEQID NO.1. The azo red dye decolorized enzyme disclosed by the invention can degrade azo red dye molecules under low hydrogen peroxide concentration and neutral condition so that decolourizing effects can be achieved; and the azo red dye decolorized enzyme can be widely applied to pollution control of industrial dye wastewater, and a method which is easy to implement, efficient and harmless to environment is provided for degradation of azo type dyes. The preparation method of the azo red dye decolorized enzyme is simple to operate, and is suitable for large-scale industrialization production.

A method of generating an antibody which inhibits a metalloenzyme is disclosed. The method comprises immunizing a subject with:(i) a synthetic zinc mimicry compound having structural and electronic properties similar to a catalytic domain of the metalloenzyme; and(ii) the metalloenzyme,Antibodies generated by this method are also disclosed as well as uses thereof.

The invention discloses a coding gene sequence with adenosine triphosphate tunneling metalloenzyme activity, a gene for coding adenosine triphosphate tunneling metalloenzyme is ZjAC1, and the ZjAC1 has a DNA sequence as shown in SEQ ID NO.1 in a sequence table. According to the invention, the coding gene sequence with adenosine triphosphate tunnel metalloenzyme activity is adopted, and the coded protein has the activity of adenylate cyclase, NTP hydrolase activity, triphosphatase activity and relatively weak pyrophosphatase activity.

The invention discloses an artificial G-quadruplex DNA metalloenzyme and application thereof. The metalloenzyme consists of ferriporphyrin and artificial G-quadruplex DNA. In the presence of K < + >, the artificial G-quadruplex DNA and ferriporphyrin are mixed in a buffer solution, and the artificial G-quadruplex DNA metalloenzyme can be obtained through in-situ synthesis by stirring. When the artificial G-quadruplex metalloenzyme is applied to olefin asymmetric cyclopropanation reaction, the artificial G-quadruplex metalloenzyme shows relatively high catalytic activity and relatively high selectivity. The artificial G-quadruplex DNA metalloenzyme does not contain precious metal components, the reaction cost is remarkably reduced, and the artificial G-quadruplex DNA metalloenzyme is mild in use condition and is a novel artificial enzyme which is environmentally friendly, high in activity, high in selectivity and excellent in stability.

The present invention provides for a genetically modified host cell capable of producing an unnatural compound, such as terpenoid, comprising a P450 enzyme comprising an artificial cofactor, and the host cell is capable of uptaking the artificial cofactor by comprising a heterologous heme transport transmembrane protein, or homologue thereof.

The invention discloses an iris lacteal heavy metal ATP enzyme transport protein I1HMA2 as well as an encoding gene and application thereof. Cloning is carried out for the first time to obtain the encoding gene (SEQ ID No: 1) of the iris lacteal heavy metal ATP enzyme transport protein I1HMA2, the gene I1HMA2 can be used for strengthening the tolerance or the enrichment capacity of plants to cadmium or improving the transport capacity of the cadmium in the plants, and thus the remediation efficiency of the plants to soil cadmium pollution is improved. Transgenic plants with the cadmium resistance and strong enrichment capacity are prepared by utilizing a gene engineering technology to be capable of growing in the cadmium polluted environment, so that the iris lacteal heavy metal ATP enzyme transport protein I1HMA2 as well as the encoding gene and the application thereof provide a new thought of cultivating efficient cadmium enrichment transgenic plants, have an important application prospect in the aspects of improving the soil cadmium pollution remediation efficiency and the ecological environment and provide a new way and technological support for remediation of heavy metal polluted soil.

The invention belongs to the field of biotechnology comprehensive utilization, and discloses a cloning and functional expression method of a typha orientalis heavy metal ATPase gene TyHMA3. The typha orientalis heavy metal ATPase gene TyHMA3 is a nucleotide sequence shown as SEQ ID NO.1 in a sequence table. The amino acid sequence is shown as SEQ ID NO.2 in a sequence table. The invention relates to a PCR primer group capable of amplifying SEQID NO.1. The primer group is as follows: HMA-F (TCTCAGTCTACTATTTAG) and HMA-R (TCAATCATAAAGAAGAAA). The invention discloses a vector for cloning the typha orientalis heavy metal ATPase gene TyHMA3. The vector is a pMD19-T vector. The gene TyHMA3 provided has the highest expression quantity in typha orientalis roots, has tissue specificity, is induced by Cd2+ and Pb2+, and is rapidly and strongly expressed. The gene participates in regulating and controlling the tolerance of typha orientalis to heavy metals. A theoretical basis is provided for a typha orientalis heavy metal strong tolerance mechanism, and a promotion effect is provided for utilization of plant heavy metal tolerance.