Sentinel lymph node rapid assay kit

A technology for rapid diagnosis of sentinel lymph nodes, applied in the field of medicine, can solve the problems of not being able to meet the needs of surgery, poor staining effect, long time, etc., and achieve the effect of less time-consuming, convenient operation and simple operation

Inactive Publication Date: 2011-02-16
常州微诊生物医药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Frozen HE staining and CK-IHC rapid pathological sections are thicker and the staining effect is not good, which cannot be completely consistent with conventional paraffin pathology; conventional IHC staining takes a long time and cannot meet the needs of surgery

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Embodiment Construction

[0060] Preferred embodiments of the present invention are described below, and it should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0061] raw material source

[0062] Tumor markers: moc-31, CD44, her-2, Mucin-1

[0063] Rat anti-human moc-31 antibody: DAKO company

[0064] Rat anti-human CD44v6-FITC antibody: Santa Cruz Company

[0065] Magnetic beads: Model Dynal beads M450, Invitrogen

[0066] Rat anti-human her-2-percp antibody (prepared using conventional antibody, labeling method)

[0067] Rat anti-human Mucin1-PE antibody (prepared using conventional antibody, labeling method)

[0068] Sentinel lymph node rapid diagnostic kit consists of four antibodies: 1. Rat anti-human moc-31 antibody, 2. Rat anti-human CD44v6-FITC antibody, 3. Rat anti-human HER-2-Percp antibody, 4. Large Mouse anti-human Mucin1-PE antibody.

[0069] Preparatio...

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Abstract

The invention discloses a sentinel lymph node rapid assay kit, which is prepared from four antibodies, namely a rat-human moc-31 antibody, a rat-human CD4-4v6-FITC antibody, a rat-human her-2-percp antibody and a rat-human Mucin1-PE antibody. The preparation and detection method comprises the following steps of: preparing sentinel lymph node single cell suspension, prewashing miltenyi; labeling the moc-31 antibody; performing cell separation; performing cell elution and performing flow cytometry. The sentinel lymph node rapid assay kit has the advantages that: the kit is convenient to operate, has short time and can finish the whole process within 60 to 70 minutes. Meanwhile, compared with intraoperational frozen HE dyeing and CK-IHC in the prior art, the sentinel lymph node rapid assay kit has obvious advantages in sensibility of diagnosing the sentinel lymph node transfer and false negative rate.

Description

technical field [0001] The invention relates to a rapid diagnosis kit for sentinel lymph nodes, which is used for the rapid diagnosis method of sentinel lymph nodes and belongs to the field of medicine. Background technique [0002] Sentinel lymph node (SLN) refers to the first lymph node drained from the primary tumor area, and is an important indicator to predict the lymph node metastasis of the tumor area. The existence of SLN indicates that the metastasis of lymph nodes in the primary tumor region is first transferred to the sentinel lymph nodes through lymphatic vessels in a predictable order, and then further transferred to distant lymph nodes. As an effective barrier, SLN can temporarily prevent further spread of tumor cells in the lymphatic channel. If there is no tumor metastasis in the SLN, in theory, other lymph nodes in the drainage area of ​​the primary tumor will not have tumor metastasis. The development of breast cancer SLNB makes it possible for clinicians...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/532
Inventor 鞠景芳卢斌峰严俊
Owner 常州微诊生物医药科技有限公司
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