Chromatographic separation material based on copolymerization on silica gel surface and preparation thereof

Abstract

A separation material based on silica gel substrate and preparation method thereof are provided. The separation material based on silica gel substrate is obtained by copolymerizing two or more silane reagents on the silica gel surface to form "non-polar / polar copolymerized stationary phase" and has the following formula, wherein NP denotes non-polar groups, which comprise C1-30 n-alkane groups, phenyl, and so on; P denotes polar groups, which comprise C1-12 n-alkane groups jointing with Cl, Br, CN, amino group, benzenesulfonic group, sulfonic group, carboxyl group, quaternary ammonium group, alcohol group, and so on. The preparation method of the above mentioned separation material comprises the following steps: acidification preprocessing of silica gel, hydrating, copolymerizing a mixture of non-polar silane reagent and polar silane reagent on the hydrated silica gel surface, filtering, washing, drying, and so on. The separation material of the present invention has both non-polar groups and polar groups, which can provide hydrophobic applied force and several kinds of polar applied forces, and thereby improve greatly the selectivity of separation and enrichment. The separation material obtained through the preparation method of the present invention has merits such as uniform and stable bonding groups on the surface, larger bonding amount and so on, and can be applied to liquid chromatogram chromatography and solid phase extraction.

Description

technical field

[0001] The invention relates to a chromatographic separation material, specifically a silica gel substrate-bonded "nonpolar / polar copolymerization stationary phase" based on silica gel surface copolymerization reaction and a preparation method thereof. Background technique

[0002] Reversed-phase high-performance liquid chromatography is the most widely used separation and analysis technique. Silica-based octadecyl-bonded stationary phase is the most commonly used separation material for reversed-phase chromatography. However, the octadecyl stationary phase can only provide a single hydrophobic force, and has poor retention and separation selectivity for polar compounds. Therefore, on the basis of conventional reversed-phase stationary phases, polar groups are introduced, and bonded stationary phases that can provide both hydrophobic and polar interaction forces are developed to supplement the polar selectivity in the reversed-phase chromatography separation...

Images