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Method for preparing fucoidan

A fucoidan sulfate and microwave technology, which is applied in the field of efficient microwave or ultrasonic assisted extraction to prepare fucoidan sulfate, achieves the effect of simple process, low cost, and enhanced immune function of the body

Inactive Publication Date: 2011-03-30
ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no literature on the use of microwave extraction technology for the extraction of fucoidan sulfate

Method used

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  • Method for preparing fucoidan

Examples

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Embodiment 1

[0021] Add 1kg of dried kelp to 40L of water, and pour it into a microwave extractor after colloid mill treatment. The microwave extraction process is as follows: microwave time 40min, microwave extraction temperature 80°C, microwave power 450W. After extraction, centrifuge at 5000rpm in a disc centrifuge for 15min, add 400g CaCl to the supernatant 2 , stirred, and placed at room temperature for 24h. After centrifugation at 5000rpm for 15min, 55% ethanol was added until the ethanol concentration in the solution was 20%, suction filtered after stirring, and the suction filtrate was concentrated to 40% of the original volume by a spherical concentration tank. Add 95% ethanol to the concentrated solution until the concentration of ethanol in the solution is 60%, centrifuge at 6000 rpm for 15 minutes after stirring. The precipitate was vacuum-dried to obtain 142 g of yellow-white fucoidan sulfate ester powder with an organic sulfate content of 19.9%.

Embodiment 2

[0023] Add 1 kg of dried wakame seaweed into 50 L of clear water, and pour it into an ultrasonic extractor after colloid mill treatment. The microwave extraction process is as follows: ultrasonic power 1000W, ultrasonic time 40min, ultrasonic extraction temperature 90°C. After extraction, centrifuge at 5000rpm for 10min in a centrifuge, add 500g CaCl to the supernatant 2 , stirred, and placed at room temperature for 24h. After centrifugation at 5000rpm for 15min, 60% ethanol was added until the ethanol concentration in the solution was 20%. After stirring, it was suction filtered, and the suction filtrate was concentrated to 30% of the original volume by a spherical concentration tank. Add absolute ethanol to the concentrated solution until the ethanol concentration in the solution is 60%, centrifuge at 6000 rpm for 15 minutes after stirring. The precipitate was vacuum-dried to obtain 151 g of yellow-white fucoidan sulfate ester powder with an organic sulfate content of 21.4...

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Abstract

The invention provides a method for preparing fucoidan, relating to the field of production and application of food additives. The method comprises the following steps of: adding distilled water according to a material to liquid ratio of 1:40-4:80 g / mL with brown algae, such as kelps, undaria pinnatifida, and the like as raw materials; treating by a colloid mill and then carrying out microwave-assisted extraction or ultrasonic wave-assisted extraction, wherein, in the microwave-assisted extraction process, the microwave time lasts 20-40min, the microwave extraction temperature is 60-80 DEG C, and the microwave power is 400-500W; and in the ultrasonic wave-assisted extraction process, the ultrasonic power is 800-100W, the microwave time lasts 20-40min, and the extraction temperature is 60-90 DEG C; and centrifuging an extracting solution, adding a proper amount of 2% CaCl2 solution into a supernatant, stirring, keeping constant temperature for 20-30h at 35-40 DEG C, centrifuging, adding 50-100% ethanol into the supernatant until the ethanol concentration of the solution is 15-20%, stirring, filtering, concentrating, adding 90-100% ethanol into a concentrated solution until the ethanol concentration of the solution is 55-60%, centrifuging again and drying a precipitate in vacuum to obtain white or yellow-white fucoidan powder.

Description

Technical field: [0001] The invention relates to a method for preparing fucoidan sulfate, in particular to a method for preparing fucoidan sulfate through efficient microwave or ultrasonic assisted extraction. Background technique [0002] Brown algae (kelp, wakame, etc.) are widely distributed along the coast of my country. In addition to being rich in alginic acid and trace elements, brown algae also contains a large amount of fucoidan sulfate (Fucoidan, also known as fucoidan). Studies in recent years have shown that fucoidan sulfate, as a polyelectrolyte, has unique biological activities in anti-tumor, anti-virus, anti-coagulation, and promotion of immunity. Its research and development has attracted more and more people. s concern. [0003] At present, a lot of research has been done on the extraction and function of fucoidan sulfate at home and abroad, and a number of invention patents have been applied for. Patent ZL98110253.0 discloses a method for preparing varia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 毛建卫吴元锋杨志祥平丽娟
Owner ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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