Quick-acting multi-microorganism composite water flush fertilizer and preparation method thereof

Abstract

The invention relates to a quick-acting multi-microorganism composite water flush fertilizer and a preparation method thereof. The quick-acting multi-microorganism composite water flush fertilizer is characterized by being prepared by mixing bacterial powder which is prepared by fermenting and culturing photosynthetic bacteria, pichiafermentanslodder, lactic acid bacteria, bifidobacterium, bacillus subtilis, bacillus licheniformis, brevibacillus laterosporus, bacillus megaterium, bacillus mucilaginosus, azotobocter chroococcum and actinomycetes. The density of the pichiafermentanslodder ACCC20319T in the quick-acting multi-microorganism composite water flush fertilizer is more than or equal to 2.0*10<8>cfu / g, and the densities of other bacteria are more than or equal to 2.0*10<9>cfu / g. The quick-acting multi-microorganism composite water flush fertilizer can improve the quality and yield of crops and shorten growth period of crops.

Description

technical field

[0001] The invention belongs to the field of biological fertilizers, and in particular relates to a quick-acting multi-strain microbial compound fertilization and a preparation method thereof. Background technique

[0002] At present, fertilization is mainly used to supply nitrogen, phosphorus, potassium macronutrients and medium and micronutrients to plants. Traditional fertilization is mainly based on inorganic elements. Extensive use of this type of flushing fertilization can cause soil compaction, deterioration of soil quality, decline of soil fertility, and decline in the quality of agricultural products. At the same time, it seriously breaks the micro-ecological balance of the soil, aggravates the occurrence of crop diseases and insect pests, and also significantly increases the use of pesticides. , which further damages the micro-ecological environment of the soil, forming a vicious circle. When traditional flushing fertilization is used, due to its ...

Examples

Embodiment 1

[0059] A preparation method for quick-acting multi-strain microbial compound fertilization, characterized in that it comprises the following steps:

[0060] (1) Preparation of composite bacteria powder

[0061] ①Preparation of various bacterial strains

[0062] Rhodopseudomonas palustris ( Rhodopaeudomonas palustris ) Preparation of ACCC 10649 bacteria solution:

[0063] Under sterile conditions, Rhodopseudomonas palustris ( Rhodopaeudomonas palustris ) ACCC 10649 was inoculated into the liquid medium, and the liquid medium was: the content of each component in 1.0L distilled water: yeast powder 3.0g, peptone 3.0g, magnesium sulfate heptahydrate 0.5g, anhydrous chloride Calcium 0.3g, pH value adjusted to 6.8-7.0, placed in a conventional light incubator, 28°C-30°C conventional light culture for 6-7 days, the number of bacteria ≥ 5.0×10 9 cfu / ml seed culture solution, then inoculated into 50 liters of sterile water containing 5% (percentage by weight) brown sugar, the i...

Embodiment 2

[0092] A preparation method for quick-acting multi-strain microbial compound fertilization, characterized in that it comprises the following steps:

[0093] (1) Preparation of composite bacteria powder

[0094] ①Preparation of various bacterial strains

[0095] According to the method for embodiment 1, the bacterium liquid of each strain is obtained;

[0096] ②Preparation of bacteria powder of various strains

[0097] According to the method of Example 1, bacteria powder is obtained from the various bacterial strains prepared in step 1., wherein, fermenting Pichia pastoris ( Pichia fermentans lodder) ACCC 20319 T Bacterial count of bacterial powder ≥ 2.0×10 8 cfu / g, the number of bacteria in the other bacterial powders is ≥2.0×10 9 cfu / g;

[0098] ② Mixing of bacteria powder of various strains

[0099] The prepared bacterial powders of various strains are by weight percentage: 12% of Rhodopseudomonas palustris bacterial powder, 12% of Pichia fermentum bacterial powde...

Embodiment 3

[0103] A preparation method for quick-acting multi-strain microbial compound fertilization, characterized in that it comprises the following steps:

[0104] (1) Preparation of composite bacteria powder

[0105] ①Preparation of various bacterial strains

[0106] According to the method for embodiment 1, the bacterium liquid of each strain is obtained;

[0107] ②Preparation of bacteria powder of various strains

[0108] According to the method of Example 1, bacteria powder is obtained from the various bacterial strains prepared in step 1., wherein, fermenting Pichia pastoris ( Pichia fermentans lodder) ACCC 20319 T Bacterial count of bacterial powder ≥ 2.0×10 8 cfu / g, the number of bacteria in the other bacterial powders is ≥2.0×10 9 cfu / g;

[0109] ② Mixing of bacteria powder of various strains

[0110] The prepared bacterial powders of various strains are by weight percentage: 11% of Rhodopseudomonas palustris bacterial powder, 11% of Pichia fermentum bacterial powder,...

technical field

[0001] The invention belongs to the field of biological fertilizers, and in particular relates to a quick-acting multi-strain microbial compound fertilization and a preparation method thereof. Background technique

[0002] At present, fertilization is mainly used to supply nitrogen, phosphorus, potassium macronutrients and medium and micronutrients to plants. Traditional fertilization is mainly based on inorganic elements. Extensive use of this type of flushing fertilization can cause soil compaction, deterioration of soil quality, decline of soil fertility, and decline in the quality of agricultural products. At the same time, it seriously breaks the micro-ecological balance of the soil, aggravates the occurrence of crop diseases and insect pests, and also significantly increases the use of pesticides. , which further damages the micro-ecological environment of the soil, forming a vicious circle. When traditional flushing fertilization is used, due to its ...

Examples

Embodiment 1

[0059] A preparation method for quick-acting multi-strain microbial compound fertilization, characterized in that it comprises the following steps:

[0060] (1) Preparation of composite bacteria powder

[0061] ①Preparation of various bacterial strains

[0062] Rhodopseudomonas palustris ( Rhodopaeudomonas palustris ) Preparation of ACCC 10649 bacteria solution:

[0063] Under sterile conditions, Rhodopseudomonas palustris ( Rhodopaeudomonas palustris ) ACCC 10649 was inoculated into the liquid medium, and the liquid medium was: the content of each component in 1.0L distilled water: yeast powder 3.0g, peptone 3.0g, magnesium sulfate heptahydrate 0.5g, anhydrous chloride Calcium 0.3g, pH value adjusted to 6.8-7.0, placed in a conventional light incubator, 28°C-30°C conventional light culture for 6-7 days, the number of bacteria ≥ 5.0×10 9 cfu / ml seed culture solution, then inoculated into 50 liters of sterile water containing 5% (percentage by weight) brown sugar, the i...

Embodiment 2

[0092] A preparation method for quick-acting multi-strain microbial compound fertilization, characterized in that it comprises the following steps:

[0093] (1) Preparation of composite bacteria powder

[0094] ①Preparation of various bacterial strains

[0095] According to the method for embodiment 1, the bacterium liquid of each strain is obtained;

[0096] ②Preparation of bacteria powder of various strains

[0097] According to the method of Example 1, bacteria powder is obtained from the various bacterial strains prepared in step 1., wherein, fermenting Pichia pastoris ( Pichia fermentans lodder) ACCC 20319 T Bacterial count of bacterial powder ≥ 2.0×10 8 cfu / g, the number of bacteria in the other bacterial powders is ≥2.0×10 9 cfu / g;

[0098] ② Mixing of bacteria powder of various strains

[0099] The prepared bacterial powders of various strains are by weight percentage: 12% of Rhodopseudomonas palustris bacterial powder, 12% of Pichia fermentum bacterial powde...

Embodiment 3

[0103] A preparation method for quick-acting multi-strain microbial compound fertilization, characterized in that it comprises the following steps:

[0104] (1) Preparation of composite bacteria powder

[0105] ①Preparation of various bacterial strains

[0106] According to the method for embodiment 1, the bacterium liquid of each strain is obtained;

[0107] ②Preparation of bacteria powder of various strains

[0108] According to the method of Example 1, bacteria powder is obtained from the various bacterial strains prepared in step 1., wherein, fermenting Pichia pastoris ( Pichia fermentans lodder) ACCC 20319 T Bacterial count of bacterial powder ≥ 2.0×10 8 cfu / g, the number of bacteria in the other bacterial powders is ≥2.0×10 9 cfu / g;

[0109] ② Mixing of bacteria powder of various strains

[0110] The prepared bacterial powders of various strains are by weight percentage: 11% of Rhodopseudomonas palustris bacterial powder, 11% of Pichia fermentum bacterial powder,...