Quick-acting multi-microorganism composite water flush fertilizer and preparation method thereof
A multi-strain and microbial technology, applied in the field of biological fertilizer, can solve problems such as breaking the soil micro-ecological balance, decreasing soil fertility, and evaporating with surface water.
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Embodiment 1
[0059] A preparation method for quick-acting multi-strain microbial compound fertilization, characterized in that it comprises the following steps:
[0060] (1) Preparation of composite bacteria powder
[0061] ①Preparation of various bacterial strains
[0062] Rhodopseudomonas palustris ( Rhodopaeudomonas palustris ) Preparation of ACCC 10649 bacteria solution:
[0063] Under sterile conditions, Rhodopseudomonas palustris ( Rhodopaeudomonas palustris ) ACCC 10649 was inoculated into the liquid medium, and the liquid medium was: the content of each component in 1.0L distilled water: yeast powder 3.0g, peptone 3.0g, magnesium sulfate heptahydrate 0.5g, anhydrous chloride Calcium 0.3g, pH value adjusted to 6.8-7.0, placed in a conventional light incubator, 28°C-30°C conventional light culture for 6-7 days, the number of bacteria ≥ 5.0×10 9 cfu / ml seed culture solution, then inoculated into 50 liters of sterile water containing 5% (percentage by weight) brown sugar, the i...
Embodiment 2
[0092] A preparation method for quick-acting multi-strain microbial compound fertilization, characterized in that it comprises the following steps:
[0093] (1) Preparation of composite bacteria powder
[0094] ①Preparation of various bacterial strains
[0095] According to the method for embodiment 1, the bacterium liquid of each strain is obtained;
[0096] ②Preparation of bacteria powder of various strains
[0097] According to the method of Example 1, bacteria powder is obtained from the various bacterial strains prepared in step 1., wherein, fermenting Pichia pastoris ( Pichia fermentans lodder) ACCC 20319 T Bacterial count of bacterial powder ≥ 2.0×10 8 cfu / g, the number of bacteria in the other bacterial powders is ≥2.0×10 9 cfu / g;
[0098] ② Mixing of bacteria powder of various strains
[0099] The prepared bacterial powders of various strains are by weight percentage: 12% of Rhodopseudomonas palustris bacterial powder, 12% of Pichia fermentum bacterial powde...
Embodiment 3
[0103] A preparation method for quick-acting multi-strain microbial compound fertilization, characterized in that it comprises the following steps:
[0104] (1) Preparation of composite bacteria powder
[0105] ①Preparation of various bacterial strains
[0106] According to the method for embodiment 1, the bacterium liquid of each strain is obtained;
[0107] ②Preparation of bacteria powder of various strains
[0108] According to the method of Example 1, bacteria powder is obtained from the various bacterial strains prepared in step 1., wherein, fermenting Pichia pastoris ( Pichia fermentans lodder) ACCC 20319 T Bacterial count of bacterial powder ≥ 2.0×10 8 cfu / g, the number of bacteria in the other bacterial powders is ≥2.0×10 9 cfu / g;
[0109] ② Mixing of bacteria powder of various strains
[0110] The prepared bacterial powders of various strains are by weight percentage: 11% of Rhodopseudomonas palustris bacterial powder, 11% of Pichia fermentum bacterial powder,...
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