Real-time fluorescence polymerase chain reaction (PCR) detection kit for screening listeria monocytogenes and detection method thereof

Abstract

The invention provides a real-time fluorescence polymerase chain reaction (PCR) detection kit for screening listeria monocytogenes. The kit mainly comprises a specific primer, an Eva Green fluorescent dye, PCR buffer solution, a deoxynucleotide triphosphate mixture and (deoxyribonucleic acid) DNA polymerases. The kit is characterized in that: the specific primer and a fluorescence probe have the following sequences: a forward primer: 5'-CGTGCATCGCCCATGTGC-3' and a reverse primer: 5'-ATCTACGAGCGTAGTCAC-3'. The kit has the main advantages that: six kinds of listeria monocytogenes can be easily screened with high sensitivity and high specificity; rapid, precise and specific detection and analysis of the six kinds of listeria monocytogenes can be realized; and standard products can be added for quantitative detection.

Description

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AI Technical Summary

Benefits of technology

This patented describes a new way to identify different types of listeriae from other organisms called Actinosynnema californicum (L.) or Leptospiraceae (Leuconostocaceae). These microorganism groups have similar DNA sequences but differ at certain positions on their chromophorins molecules. By comparing these two nucleus structures they may find ways to distinguish them based upon how well each structure looks under normal conditions. They found that there were 66 distinct forms within seven strain families - three yeast genera, five leukocytes, four tissue cells, eight neurons, and ten spongious creatures – all containing ribonucleides and amino acids associated with proteins involved in cellular metabolites production. Overall, this technique allows researchers to quickly and easily discover important biological materials like those related to neurology diseases such as Parkinson' disease and Alzheimer’s disease.

Problems solved by technology

This patents discuss different techniques used by laboratories during identifying specific types of bacterae called Listeriae or Microcystis aeruginosa. These traditional ways involve culturing them from their natural environments until reaching maturity, but these procedures take up too much space at room temperature. Therefore, new technologies like molecular biology analysis were developed recently to speed up the process while reducing costs associated with testing each type separately.

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