Feed additive rich in lysozyme

A feed additive, lysozyme technology, applied in the fields of food safety, enzyme preparations, and feed additives, can solve problems such as residues, drug-resistant drugs, and dysbiosis of animal gastrointestinal tract, so as to reduce water absorption and enhance immunity. strength, and the effect of promoting body tissue repair

Inactive Publication Date: 2011-04-27
杭州普爱生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the widespread use of antibiotics in feed additives, their disadvantages have become increasingly prominent, such as causing animal gastrointestinal flora imbala

Method used

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  • Feed additive rich in lysozyme
  • Feed additive rich in lysozyme
  • Feed additive rich in lysozyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0029] Implementation Case 1 Preparation of Lysozyme Feed Additive

[0030] Based on the enzyme activity content in the additive, lysozyme 200000IU / g, cellulose CMCase 5500IU / g, xylanase 145000IU / g, acid protease 5000IU / g, β-glucanase 25000IU / g, pectinase 8000IU / g combination, prepared as a compound enzyme feed additive.

[0031] Its preparation method is:

[0032] 1) Compound enzyme preparation: according to the enzyme activity content of the enzyme, the enzyme activity is converted into the required mass, and the corresponding mass of lysozyme, cellulase, xylanase, etc. is weighed.

[0033] 2) Mixed packaging: mix the weighed compound enzyme evenly, and seal the package.

Embodiment example 2

[0034] Example 2 Inhibitory effect of lysozyme-rich feed additive on Escherichia coli

[0035] Detection by drug-sensitive disc method:

[0036] (1) Production of drug-sensitive paper sheets

[0037] Take a qualitative filter paper with good quality, and punch it into a small circular piece of paper with a diameter of 6mm with an ordinary puncher. Take 50 pieces of round paper and put them into a clean and dry vial, and wrap them with a single layer of kraft paper. After autoclaving at 15 pounds for 15-20 minutes, place in a 37°C incubator for several days to completely dry. Based on the saturated water absorption of each piece of paper is 0.01ml, add 0.5ml of liquid medicine to the vial containing 50 pieces of paper, and stir it from time to time to make the filter paper evenly absorb the liquid medicine. incubator overnight. After drying, it should be sealed and stored in a dark and dry place.

[0038] (2) Preparation of liquid medicine

[0039] Dissolve and dilute the...

Embodiment example 3

[0048] Example 3 Inhibitory effect of lysozyme-rich feed additive on Staphylococcus aureus

[0049] The test method is the same as above (note: the test bacteria liquid is Staphylococcus aureus).

[0050] Test results: see Table 4

[0051] Table 4: Antimicrobial susceptibility test results of Staphylococcus aureus

[0052]

[0053] Conclusion: It can be seen that the feed additive is highly sensitive to Staphylococcus aureus.

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PUM

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Abstract

The invention aims to provide a feed additive rich in lysozyme. Each gram of feed additive comprises the following substances: 200,000IU of lysozyme, 5,500IU of cellulose CMC enzyme, 145,000IU of xylanase, 5,000IU of acid protease, 25,000IU of beta-glucanase, and 8,000IU of pectinase. The feed additive has the effects of killing bacteria, protecting environment, enhancing immunity of animals, reducing medicine residue, improving production performance and the like.

Description

technical field [0001] A feed additive rich in lysozyme, the invention relates to the fields of feed additives, food safety and enzyme preparations, specifically a novel green feed additive, which has the characteristics of sterilization and improved production performance, can replace or synergize antibiotics, reduce Drug residues in animal products. Background technique [0002] Antibiotics have been used as feed additives in the aquaculture industry for more than 60 years, and they have played a positive role in preventing animal diseases and improving the yield and quality of the aquaculture industry. However, with the widespread use of antibiotics in feed additives, their disadvantages have become increasingly prominent, such as causing animal gastrointestinal flora imbalance, drug resistance and drug residues, etc., causing a series of public safety, environmental protection and quality of livestock and poultry products. aspects of the problem. In view of this, the v...

Claims

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Application Information

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IPC IPC(8): A23K1/165A23K20/189
Inventor 李龙
Owner 杭州普爱生物科技有限公司
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