Method for extracting mulberry root ketone C

An extraction method and technology of sanggenone, applied in the field of extracting sanggenone C, can solve the problems of difficult large-scale preparation and high cost, and achieve the effects of low cost, easy scale-up production, and small processing capacity

Inactive Publication Date: 2011-05-04
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent is also difficult to prepare in large quantities, and the cost is also high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] After the Cortex Mori is dried, crush small particles, take 5kg, put it into a small ultrasonic extraction tank, configure a lime aqueous solution with a pH of 9, measure 75kg, 50kg and 30kg of liquid respectively, and add them to the extraction tank for ultrasonic extraction for 2 hours, 1 hour, and 1 hour. Turn on the power of 2kw, combine the three extracts, adjust the pH to 8 with hydrochloric acid and filter. Take 7LAB-8 resin and put it into a resin column (diameter-to-height ratio 1:15), sequentially treat it with acid and alkali, wash it with water and add the extract to the macroporous resin column at a flow rate of 5L / h. After the adsorption is completed, the water-based sugar reaction Negative, then take 56L of 60% ethanol for elution, collect the eluent to recover ethanol under reduced pressure, place the concentrated solution to precipitate at pH 1, and filter to obtain 50 g of precipitate. Reflux the precipitate with 500ml of acetone to dissolve, filter an...

Embodiment 2

[0019] After the Cortex Mori is dried, crush the small particles, take 5kg, put it into a small ultrasonic extraction tank, configure a lime aqueous solution with a pH of 10, measure 50kg, 40kg and 30kg of liquid respectively, and add them to the extraction tank for ultrasonic extraction for 1 hour, 1 hour, and 1 hour. Turn on the power of 2kw, combine the three extracts, adjust the pH to 7 with hydrochloric acid and filter. Take 6L of HPD100 resin and put it into a resin column (diameter-to-height ratio 1:20), sequentially treat it with acid and alkali, wash it with water and add the extract to the macroporous resin column at a flow rate of 8L / h. After the adsorption is completed, the water-based sugar reaction is negative , and then take 30L of 75% ethanol for elution, collect the eluent to recover ethanol under reduced pressure, place the concentrated solution to precipitate at pH 4, and filter to obtain 65g of precipitate. Dissolve the precipitate with 400ml of acetone und...

Embodiment 3

[0021] After the Cortex Mori is dried, crush the small particles, take 10kg, put it into a small ultrasonic extraction tank, configure a lime aqueous solution with pH 11, measure 100kg, 100kg and 60kg of liquid respectively, add it to the extraction tank for ultrasonic extraction for 1 hour, 1 hour, and 1 hour, Turn on the power of 4kw, combine the three extracts, adjust the pH to 7 with sulfuric acid and filter. Take 15L of HZ818 resin and put it into a resin column (diameter-to-height ratio 1:15), add the extract to the macroporous resin column at a flow rate of 15L / h. Ethanol was recovered by dehydration under reduced pressure, the concentrated solution was placed to precipitate at pH 3, and 96 g of precipitate was obtained by filtration. Dissolve the precipitate with 700ml of acetone under reflux, filter and add to a silica gel column (diameter-to-height ratio 1:15, silica gel is 200-300 mesh neutral silica gel, weight 2kg), take 10kg of 20% acetone solution for elution, a...

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Abstract

The invention relates to a method for extracting mulberry root ketone C. The process method provided by the invention comprises the following steps: crushing white mulberry root-bark into small grains; carrying out ultrasonic extraction for 2-3 times by utilizing an alkali aqueous solution the pH value of which is 9-11; regulating the pH value to 7-8 by the extraction; absorbing by adding macroporous resin; eluting by an ethanol solution; recycling ethanol after eluting; regulating the acidity, and precipitating; dissolving the precipitates in acetone, and then, adding a silicagel column for separating; carrying out gradient elution by an acetone aqueous solution; concentrating and crystallizing; recrystallizing by a methanol chloroform solution; and drying to obtain a product. The method provided by the invention has the advantage of simple operation and solves the difficulty that the conventional silicagel column separation can not expand the production, and the content of the obtained product is relatively high.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, in particular to a method for extracting mulberry ketone C from Cortex Morus alba. Background technique: [0002] Morus alba L. is the dry root bark of the Moraceae plant Morus alba L. Excavate the root when the leaves fall in late autumn and before germination in the next spring, scrape off the yellowish-brown rough skin, cut it longitudinally, peel off the root bark, and dry it in the sun. Lung heat cough and asthma. Sweet and cold in nature, descending in nature, mainly enters the lung meridian, can clear lung fire and relieve water vapor in the lungs to relieve asthma. Treat cough and asthma due to lung heat, body edema, edema of the face and skin, fullness and shortness of breath, difficulty urinating. In addition, it also has the function of clearing the liver, lowering blood pressure and stopping bleeding. . [0003] According to modern medical research, Cortex Morus alba contains a varie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04
Inventor 刘东锋吴艳波杨成东
Owner NANJING ZELANG MEDICAL TECH
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