Method for enhancing tulip tissue culture seedling

A technique for tissue culture seedlings and tulips, which is applied to the field of plant tissue culture, can solve the problems of small bulbs, low survival rate of tissue culture seedlings, and hinder the rapid propagation of tulips by tissue culture, and achieves the effects of saving agar, reducing pollution rates and reducing costs.

Active Publication Date: 2011-06-15
DASHUN INT FLOWER
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AI Technical Summary

Problems solved by technology

However, the small bulbs and low survival rate of tissue culture seedlings have become a problem hindering the rapid propagation of tulip tissue culture

Method used

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  • Method for enhancing tulip tissue culture seedling
  • Method for enhancing tulip tissue culture seedling

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Experimental program
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Embodiment Construction

[0007] Test method: select tulip small bulbs with a diameter of 0.4 to 0.6 cm and rich root tissue cultured seedlings as test materials. The culture conditions are temperature of 23±2°C, light of 1500-2000Lx, and humidity of 75%-80%. 60 tissue cultured seedlings were treated for each treatment, and the results were counted after 40 days.

[0008] Evaluation indicators and measurement methods:

[0009] The electronic balance measures the total mass of bulb seedlings and the mass of roots, bulbs and leaves;

[0010] Vernier calipers measure the diameter of the bulb;

[0011] Measure the length of leaves and roots with a ruler;

[0012] Count the number of roots, bulbs, and leaves.

[0013] Test group 1

[0014] The tulip tissue culture seedlings were transferred to the MS liquid medium supplemented with 2 mg paclobutrazol per liter for culture by the above method.

[0015] Test group 2

[0016] The tulip tissue culture seedlings were transferred to the MS liquid medium su...

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Abstract

The invention discloses a method for enhancing a tulip tissue culture seedling, which belongs to the field of plant tissue culture methods, in particular to the field of tissue culture rapid propagation of flowering bulbs. The method comprises the following step of: transferring the tulip tissue culture seedling into an MS (Multi Spore) liquid culture medium into which 2-8 mg/L of paclobutrazol is added for culturing, wherein the culture temperature is 23+/-2 DEG C, the illumination is between 1,500 Lx and 2,000 Lx and the humidity is between 75 percent and 80 percent. The invention has the advantages that: liquid culture is adopted, and paclobutrazol is added instead of the conventional method in which a bulb expands due to the increment in sugar concentration, so that the pollution rateis lowered, the agar is saved simultaneously, and the cost is lowered. During transplanting, the root of the tissue culture seedling is easy to clean. By adopting the method, a robust tissue culture seedling with a large bulb and a dwarf plant is obtained, the diameter of the bulb is up to 1.32 centimeters, and the mass is up to 0.93 gram.

Description

technical field [0001] The invention relates to the field of plant tissue culture methods; in particular to the field of tissue culture and rapid propagation of bulbous flowers. Background technique [0002] Tulip (Tulipa gesneriana) Liliaceae, tulip genus. Perennial bulbous flowers, with various flower shapes, rich flower colors, and tall and straight plant shapes, are deeply loved by people. The natural reproduction speed of tulip is slow, the reproduction coefficient is low, and the bulb virus is easy to accumulate and cause the bulb to degenerate or die gradually. In order to solve this problem, the tissue culture technology of tulip plant has developed rapidly. However, the small bulbs and low survival rate of tissue cultured seedlings have become a difficult problem hindering the rapid propagation of tulip tissue culture. Contents of the invention [0003] In order to solve the technical problems of small tulip tissue culture bulbs, weak seedling growth and low tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨铁顺
Owner DASHUN INT FLOWER
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