A kind of culture medium for bletilla striata tissue culture

A culture medium and technology of Bletilla striata group, applied in the fields of application, plant cells, horticulture, etc., can solve the problems of low transplanting survival rate, long domestication time, slow growth, etc., and achieve high transplanting survival rate, less variation, and seedlings root robust effect

Active Publication Date: 2017-07-14
康美药业(昆明)种质资源有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are problems in the cultivation of traditional medium, such as easy to produce mutant seedlings, weak bulbs, slow growth, long domestication time, and low survival rate of transplanting.

Method used

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  • A kind of culture medium for bletilla striata tissue culture

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Embodiment 1

[0024] An improved MS medium for tissue culture of Bletilla striata: the formula is shown in Table 1.

[0025] The improved MS culture medium of table 1 embodiment 1

[0026] name Dosagemg / L potassium nitrate 1900 ammonium nitrate 1650 Calcium Chloride Dihydrate 440 Magnesium Sulfate Heptahydrate 370 Potassium dihydrogen phosphate 170 potassium iodide 0.083 boric acid 6.2 Manganese Sulfate Tetrahydrate 22.3 Zinc sulfate heptahydrate 8.6 Sodium molybdate 0.25 Copper Sulfate Pentahydrate 0.025 Cobalt chloride 0.025 ferrous sulfate 27.8 Ethylenediaminetetraacetic acid 24.3 Inositol 100 niacin 0.8 Pyridoxine Hydrochloride 0.8 Thiamine hydrochloride 0.8 Glycine 3 pH 5.8

Embodiment 2

[0028] An improved MS medium for tissue culture of Bletilla striata: the formula is shown in Table 2.

[0029] The improved MS culture medium of table 2 embodiment 2

[0030] name Dosagemg / L potassium nitrate 1890 ammonium nitrate 1660 Calcium Chloride Dihydrate 445 Magnesium Sulfate Heptahydrate 375 Potassium dihydrogen phosphate 160 potassium iodide 0.75 boric acid 5 Manganese Sulfate Tetrahydrate 24 Zinc sulfate heptahydrate 10 Sodium molybdate 0.2 Copper Sulfate Pentahydrate 0.03 Cobalt chloride 0.03 ferrous sulfate 30 Ethylenediaminetetraacetic acid 26 Inositol 95 niacin 1 Pyridoxine Hydrochloride 1 Thiamine hydrochloride 1 Glycine 4 pH 6.0

Embodiment 3

[0032] An improved MS medium for tissue culture of Bletilla striata: the formula is shown in Table 3.

[0033] The improved MS culture medium of table 3 embodiment 3

[0034] name Dosagemg / L potassium nitrate 1895 ammonium nitrate 1655 Calcium Chloride Dihydrate 442 Magnesium Sulfate Heptahydrate 373 Potassium dihydrogen phosphate 165 potassium iodide 0.8 boric acid 5.5 Manganese Sulfate Tetrahydrate 23.6 Zinc sulfate heptahydrate 10 Sodium molybdate 0.2 Copper Sulfate Pentahydrate 0.03 Cobalt chloride 0.028 ferrous sulfate 29 Ethylenediaminetetraacetic acid 25 Inositol 98 niacin 0.9 Pyridoxine Hydrochloride 0.9 Thiamine hydrochloride 0.9 Glycine 3.5 pH 5.9

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Abstract

The invention provides a culture medium for bletilla striata tissue culture. The culture medium consists of improved MS culture medium: 1890mg / L to 1900mg / L of potassium nitrate, 1650mg / L to 1660mg / L of ammonium nitrate, 440mg / L to 445mg / L of CaCl2.2H2O, 370mg / L to 375mg / L of magnesium sulfate heptahydrate, 165mg / L to 170mg / L of monopotassium phosphate, 0.75mg / L to 0.83mg / L of KI, 0.025mg / L to 0.03mg / L of cobalt chloride, 27.8mg / L to 30mg / L of ferrous sulfate, 24.3mg / L to 26mg / L of ethylenediaminetetraacetic acid, 95mg / L to 100mg / L of inositol, 0.8mg / L to 1mg / L of niacin, 0.8mg / L to 1mg / L of pyridoxine hydrochloride, 0.8mg / L to 1mg / L of thiamine hydrochloride and 3mg / L to 4mg / L of glycine. Bletilla striata grown by the culture medium has the characteristics of strong roots, big corms, less variation phenomenon, high transplanting survival rate and fast growth.

Description

technical field [0001] The invention relates to the field of bletilla striata tissue culture, in particular to a culture medium for bletilla striata tissue culture. Background technique [0002] Bletilla striata is a perennial herbaceous bulbous plant with a plant height of 18-60 cm. It is mainly distributed in China, Japan and northern Myanmar. The main flowering period is in spring, but depending on the climate of each place, it may bloom from late winter to early summer. Bletilla striata has a wide range of medicinal value and garden value. Bletilla striata is mainly used to astringe and stop bleeding, reduce swelling and promote muscle growth. It can be used as a potted plant for indoor viewing, or as a decoration in a relatively shaded flower bed, flower border or corner of a courtyard. [0003] Utilizing tissue culture technology to quickly cultivate Bletilla striata seedlings is the most powerful means of artificially propagating Bletilla striata seedlings at present...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 许冬瑾
Owner 康美药业(昆明)种质资源有限公司
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