Yeast grease and preparation method and application thereof

A yeast and oil technology, applied in the fields of food, medicine and bioengineering, can solve the problem that the production technology stays in the laboratory stage.

Inactive Publication Date: 2013-07-31
BINZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although oleaginous yeast is a good source of new oils and fatty acids, its production technology is only at the laboratory stage, and a lot of research work is still needed to achieve the purpose of industrial production and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Extraction of brewer's yeast oil

[0096] 2000g of brewer's yeast mud was washed with water for 3 times by repeated suction, filtration, and dried at a low temperature of 60°C to obtain 178g of dry yeast. Pulverize, weigh 100g of yeast powder, add 500ml of n-hexane, extract under reflux for 1 hour, filter with suction, collect the filtrate, add 400ml of n-hexane to the filter residue and repeat the extraction once, and filter with suction. The two filtrates were combined, and n-hexane was recovered by distillation to obtain yeast oil 6.37, wherein the triterpene content (calculated as squalene) was 2.12 g. Redissolve grease with 200ml ether, suction filtration, collect the filtrate, distill and recover ether, and continue to heat in a water bath and evaporate the ether to obtain 5.72g of refined grease, wherein the triterpene content (calculated by squalene) is 2.01 g g.

Embodiment 2

[0098] Extraction of red yeast oil

[0099] (1) Yeast raw material pretreatment

[0100] The fermented Rhododendron glutinosa fermented liquid is pressed into a three-legged centrifuge, rinsed with water while centrifuging, and dried; or washed with a plate-and-frame filter press to remove excess water, collect the bacterial cells, and fluidize them by vibration. Dry in a bed dryer or a flat dryer to make the water content about 8%-10% (w / w), and enter the steaming and frying process of the yeast raw material;

[0101] (2) Steamed and fried mushrooms

[0102] Load the fungus material treated in step 1 with a charging coefficient of 0.8-0.9, spray water and direct steam into the fungus material, and make the fungus material fully contact with water and moisten evenly by stirring, and the stirring speed is 25- 40r / min, turn off the exhaust, heat the fungus material to a temperature of 95-100 °C, make the surface of the fungus material fully penetrate and steam, and the moisture ...

Embodiment 3

[0113] Supercritical CO Refining of Rhododendron viscosus oil 2 Extraction

[0114] Example 2 700g of red yeast oil and fat obtained, wherein 20mL of absolute ethanol was added and stirred, and the alcohol-oil mixture was added to the extraction kettle at one time, and the CO 2 The storage tank is cooled to 4°C, and the extraction kettle and the analysis kettle are heated respectively. When the temperature reaches 40°C and 32°C, and the pressure of the extraction kettle and the analysis kettle reaches 35MPa and 10MPa, respectively, keep the static pressure for 5-25min, and turn off the CO. 2 Cylinder, start circulating extraction, adjust CO 2 The flow rate reaches 20L / h, the extraction at constant temperature and pressure is performed for 1.5h, and the refined oil is obtained from the analytical kettle; the yield is 96.27%; the oil is subjected to supercritical CO 2 After extraction and purification, the content of unsaturated fatty acids is increased by 10-25%.

[0115] Af...

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Abstract

The invention relates to the technical field of biological engineering and the fields of food and medicaments, particularly provides a method for extracting refined yeast grease from yeast, particularly waste saccharomyces cerevisiae and oil production yeast, and further provides soft capsules prepared by compounding the yeast grease, vegetable grease rich in necessary fatty acid for a human body and anti-oxidation grease and a preparation method thereof. The obtained yeast grease composite soft capsules have the effects of reducing serum cholesterol and reducing blood fat; the method for producing the yeast grease by using the waste yeast has powerful results of improving the additional value of the waste yeast and generating higher economic benefit; and meanwhile, by comprehensive utilization of the waste yeast, the problem that wastes pollutes environment is solved, and the requirement of circular economy development is met.

Description

technical field [0001] The present invention relates to the technical field of bioengineering and the field of food and medicine, in particular to a kind of oil extracted by using yeast as a raw material, and a method for preparing soft capsules by using the oil, in particular to a soft capsule prepared by using oil extracted from beer waste yeast and its preparation method. Background technique [0002] Brewer's yeast is a certain species of Saccharomyces cevevisiae, which contains a variety of biologically active substances, mainly about 50% of protein, 6-8% of nucleic acid, about 10% of polysaccharide, about 6-9% of Fat, also rich in B vitamins, vitamin D 2 In addition, it also contains a variety of coenzymes and other physiologically active substances with high economic value. [0003] Oleaginous yeast is a yeast species with a cell oil accumulation of more than 20%, and some species such as Pulmonary yeast and oleaginous yeast have an oil content of more than 60%. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P5/02A23D7/00A23D7/02A23D7/04C11B1/06C11B1/10C11B3/12
CPCY02E50/343Y02P20/54
Inventor 王宝琴徐泽平
Owner BINZHOU UNIV
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