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Method for rooting culture,seedling adaptation and transplantation of alpine rose tissue-cultured seedling in greenhouse

A technology of rooting culture and tissue culture seedlings, which is applied in the field of plant cultivation to achieve the effects of improving production efficiency, shortening time for seedling cultivation, and reducing production costs

Inactive Publication Date: 2011-07-13
宜昌市科力生实业有限公司
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

These methods have improved the transplanting survival rate and seedling quality of alpine rhododendron tissue culture seedlings to a certain extent, but have not fundamentally overcome these defects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The steps of this method are as follows:

[0065] (1) Emergence of bottle seedlings

[0066] The shoots of Nova zember, an excellent alpine rhododendron variety, were selected as the exosomes. Prepare medium, etc. according to normal procedures, and handle exosomes. The buds of Rhododendron alpine were induced through the primary culture, and then the aseptic buds with axillary buds obtained through the primary culture were transferred to the differentiation medium for proliferation and culture.

[0067] After the proliferation reaches a certain amount, the strong seedlings are cultivated, and then the strong seedlings with a length of 4 cm, more than 4 leaves, dark green leaves, and robust seedlings are ready to be moved into the greenhouse.

[0068] Before moving into the greenhouse, adjust the temperature control system and light control system in the greenhouse. When the seedlings are moved into the greenhouse, the temperature is controlled at 18-22°C during the ...

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PUM

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Abstract

The invention relates to a method for rooting culture and seedling adaptation and transplantation of alpine rose in greenhouse, comprising the following steps of: germinating bottled seedlings, rooting through water planting, transplanting rooted seedlings and taking management after transplantation, and the like. The method disclosed by the invention not only shortens the time of seedling culture, but also increases the transplantation survival rate to more than 92%, largely reduces the production cost, and improves the production benefits. Moreover, the method disclosed by the invention can promote early growing of new roots and form strong fibril root systems; moreover, culture mediums can be cleaned easily during transplantation, thereby overcoming the defect that the survival rate is affected because the root fibril is easy to break when solid culture mediums are cleaned.

Description

【Technical field】 [0001] The invention belongs to the technical field of plant cultivation. More specifically, the present invention relates to a method for rooting cultivation and hardening and transplanting of rhododendron alpine tissue culture seedlings in a greenhouse. 【Background technique】 [0002] Alpine rhododendron is a large category of rhododendron resources, belonging to evergreen rhododendron. It has beautiful tree shape, unique flower shape, bright colors, and very high ornamental value. It is recognized as a high-end potted flower. [0003] The emergence rate of Rhododendron alpine sexually propagates through seeds is low, mainly relying on cuttings, and cutting propagation is affected by the material of the mother plant and the breeding season, and often cannot meet the needs of the market. Therefore, except for a small part of the breeding of rhododendron alpine seedlings by grafting and cuttings, most of the seedlings are produced by tissue culture. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00A01G31/00
Inventor 邓国文徐年声
Owner 宜昌市科力生实业有限公司
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