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Detecting test paper

A technology for detecting test strips and reaction layers, which is applied in the field of clinical testing, can solve problems such as contact with testers, pollute testing instruments, and pollute the environment, and achieve stable test results

Active Publication Date: 2011-07-20
ACON BIOTECH (HANGZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the amount of blood sample added is large, when the liquid-saturated reaction layer can no longer absorb the excess sample, the excess blood sample will overflow the test paper, pollute the environment or come into contact with the tester.
Excess blood will also gather on the reaction reagent layer to form droplets, which not only affects the interpretation of the results by optical and other detection instruments, but also seriously contaminates the detection instruments

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 A kind of dry chemical test paper of the present invention

[0046] Dry chemical detection test paper of the present invention, as Figure 4 As shown, the reaction layer 12 is adhered and supported on the bottom plate 11, and the other side of the reaction layer relative to the bottom plate is covered with a sample application layer 13, and a sample application port 14 is provided on the sample application layer. Both ends of the sample application layer 13 partially cover the liquid-locking component 15 . There is a detection window 16 at the bottom plate opposite to the reaction layer 12 . The adhesive sheet 17 is continuously adhered and pressed against the sample application layer, the liquid-locking part and the bottom plate. The bottom plate 11 is made of PET material with a thickness of 0.35 mm. The reaction layer 12 is made of nylon membrane material, with a thickness of 0.05mm. The sample loading layer 13 is made of glass fiber polymer material...

Embodiment 2

[0047] Embodiment 2 Different sample additions detect cholesterol on the dry chemical test paper of the present invention

[0048] A whole blood solution was prepared with a cholesterol concentration of 150 mg / dl, wherein the sample had a hematocrit (HCT) concentration of 42%. 15 μl, 20 μl, 30 μl and 40 μl of the above whole blood solution were respectively taken and dropped onto the dry chemical test paper described in Example 1 of the present invention for detection. When the amount of the sample reaches 20 μl, the absorbance of the reaction layer test paper of the dry chemical test paper described in Example 1 of the present invention reaches saturation. Sample volumes of 30 μl and 40 μl were considered excessive. The test results show that the dry chemical test paper of the present invention is not affected by the amount of sample added, and can well absorb excess samples. The test results are stable and accurate. The test results are shown in Table 1.

[0049] Table 1...

Embodiment 3

[0051] Example 3 Different sample volumes are affected by the HCT concentration experiment

[0052] Whole blood solutions with hematocrit (HCT) of 30%, 42%, and 55% were prepared respectively, and were dropped onto the dry chemical test paper described in Example 1 of the present invention for detection. The sample volumes were 15ul, 20ul, 30ul and 40ul respectively. When the amount of the sample reaches 20 μl, the absorbance of the reaction layer test paper of the dry chemical test paper described in Example 1 of the present invention reaches saturation. Sample volumes of 30 μl and 40 μl were considered excessive. The results show that the use of the dry chemical test paper of the invention is not affected by the hematocrit or the amount of sample added, and the detection results are stable and consistent. The results are shown in Table 2 to Table 4.

[0053] Table 2

[0054]

[0055] table 3

[0056]

[0057] Table 4

[0058]

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Abstract

The invention relates to a detecting test paper, in particular to a dry chemical test paper for detecting the content of the analyzed object in the blood sample. The detecting test paper comprises a sample adding layer, a reacting layer, a base plate and a liquid-locking component, wherein the sample adding layer, the reacting layer and the base plate are vertically arranged from the top to bottom; at least one side of the reacting layer is provided with the liquid-locking component; and the speed of the liquid-locking component for absorbing the liquid is less than or equal to the speed of the reacting layer for absorbing the liquid. As the liquid-locking component is capable of absorbing the redundant liquid sample, the redundant sample does not flow out of the range of the test paper, and the phenomenon of polluting the environment or the sample contacting to the detector is avoided.

Description

technical field [0001] The invention relates to the technical field of clinical detection, in particular to a dry chemical test paper for detecting biochemical indicators in blood samples. Background technique [0002] Currently, methods for detecting the presence or amount of a certain component in blood include liquid reagent testing and dry chemical strip testing. Dry chemical test paper has the characteristics of convenient use, simple operation and quick results. [0003] According to the flow path of the liquid sample on the dry chemical test paper, the dry chemical test paper can be divided into various types, and the vertical vertical detection test paper is a kind of dry chemical test paper. The vertical vertical detection test paper includes stacking the carriers with the reaction reagents on top of each other, the liquid sample flows through each reaction reagent layer from top to bottom, and finally displays the detection results. For example, U.S. Patent No. 4...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52
Inventor 商涛王春明
Owner ACON BIOTECH (HANGZHOU) CO LTD
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