Probiotic bacterium food supplement, and preparation method and use thereof
A dietary supplement, a technology for children to take, applied in the field of probiotic dietary supplements and their preparation
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preparation example Construction
[0032] The preparation process of the probiotic dietary supplement of the present invention is carried out under the conditions that the room temperature is kept at 20±5° C., the indoor relative humidity is ≤50%, and the environmental cleanliness is at 100,000 grades. Too high indoor temperature and relative humidity will affect the viable bacteria content of the preparation.
[0033] The total content of probiotics in the probiotic dietary supplement finally obtained by the present invention is more than or equal to 10 9 pcs / g (production day).
[0034] According to the research of the inventors, the above-mentioned probiotic dietary supplements can be processed into probiotic powder or probiotic microcapsules, and then processed into capsules or powders, or granulated to make granules, or granulated and compressed to make tablets Ingredients and other dosage forms.
[0035] The water content of the probiotic dietary supplement of the present invention should be controlled ...
Embodiment 1
[0038] Preparation of Bifidobacterium infantis powder
[0039] 1. Take the Bifidobacterium infantis strains used for production, activate them with liquid MRS medium, and culture them at 37°C±1.0°C for 36-48h; use the solid MRS medium to streak the activated bacteria liquid, 37°C±1.0°C Cultivate for 48-72 hours; select a single colony on the plate to streak with solid MRS medium, culture at 37°C±1.0°C for 48-72h, transfer after cultivation, or store in a refrigerator at 4°C for later use. Select 1 ring of slant strains and insert them into the test tube of liquid MRS medium for 36-48 hours at 37°C±1.0°C; inoculate the above liquid culture into 500ml liquid MRS medium at 37°C±1.0°C for 36 hours according to the inoculum size of 2%. -48h, get first-grade Bifidobacterium infantis seeds for production.
[0040]2. Prepare MRS medium, sterilize at 121°C-132°C for 10-15 minutes, then cool down to the inoculation temperature of 36-40°C, inoculate the first-grade Bifidobacterium infan...
Embodiment 2
[0043] Preparation of Microcapsules of Bifidobacterium infantis
[0044] 1. Take the Bifidobacterium infantis strains used for production, activate them with liquid MRS medium, and culture them at 37°C±1.0°C for 36-48h; use the solid MRS medium to streak the activated bacteria liquid, 37°C±1.0°C Cultivate for 48-72 hours; select a single colony on the plate to streak with solid MRS medium, culture at 37°C±1.0°C for 48-72h, transfer after cultivation, or store in a refrigerator at 4°C for later use. Select 1 ring of slant strains and insert them into the test tube of liquid MRS medium for 36-48 hours at 37°C±1.0°C; inoculate the above liquid culture into 500ml liquid MRS medium at 37°C±1.0°C for 36 hours according to the inoculum size of 2%. -48h, get first-grade Bifidobacterium infantis seeds for production.
[0045] 2. Prepare MRS medium, sterilize at 121°C-132°C for 10-15 minutes, then cool down to the inoculation temperature of 36-40°C, inoculate the first-grade Bifidobact...
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