Quality control method of pulean preparation

A detection method and Pulean technology, applied in the field of quality control of Pulean preparations, can solve the problems of poor quantification and lack of α-linolenic acid, and achieve control of internal quality, good reliability, and precise testing. high degree of effect

Active Publication Date: 2013-04-24
浙江现代中药与天然药物研究院有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, there is no content determination of α-linolenic acid in the quality standard of Pulean preparations at present, and the determination of unsaturated fatty acid content in pollen only measures the relative content of each fatty acid in fatty oil, which cannot fully clarify the problem
However, due to the fact that the unsaturated fatty acids in pollen are easy to interact with each other in actual detection, it cannot be quantified well.

Method used

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  • Quality control method of pulean preparation
  • Quality control method of pulean preparation
  • Quality control method of pulean preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1: Assay

[0037] 1. Method

[0038] (1) Preparation of test solution: Take 20 Pulean capsules (batch number: 20090402), grind finely, weigh 0.5g, accurately weigh, place in a stoppered conical flask, add 50ml petroleum ether (60-90 ℃) Ultrasonic extraction for 1 hour, suction filter with triangular suction filter, wash the funnel and filter paper with a small amount of petroleum ether, put the filter residue and filter paper in an Erlenmeyer flask, continue to extract 2 times, and use 50ml petroleum ether for the second ultrasonic extraction for 1 hour. The third ultrasonic extraction with 30ml petroleum ether for 0.5 hours, suction filtration after each extraction, wash the funnel and filter paper with a small amount of petroleum ether, combine the filtrate, recover the solvent under reduced pressure at 60℃ to dryness, add sulfuric acid methanol-benzene to the residue (weight ratio 1:1) 10ml, reflux in a water bath at 60°C for 30 minutes, let cool, add 10ml of n-h...

Embodiment 2

[0045] Example 2: Assay

[0046] 1. Method

[0047] (1) Preparation of the test solution: Take 20 tablets of Pro'an (batch number: 20090422), grind them into fine pieces, weigh 0.5g, accurately weigh, and place in a stoppered conical flask, add 50ml petroleum ether (60- 90℃) Ultrasonic extraction for 1 hour, then filter with triangular suction filter, wash the funnel and filter paper with a small amount of petroleum ether, put the filter residue and filter paper in an Erlenmeyer flask, continue the extraction 2 times, and use 50ml petroleum ether for the second ultrasonic extraction for 1 hour For the third time, use 30ml petroleum ether for ultrasonic extraction for 0.5 hours. After each extraction, filter with suction. Wash the funnel and filter paper with a small amount of petroleum ether. Combine the filtrate. Recover the solvent under reduced pressure at 60℃ to dryness. Add sulfuric acid methanol-benzene to the residue. Ratio 1:1) 10ml, reflux in a water bath at 60℃ for 30 mi...

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Abstract

The invention provides a quality control method of a pulean preparation. The quality of the pulean preparation is controlled through measuring the content of alpha-linolenic acid in the pulean preparation; the quantity of needed pulean preparation is less; the content of the alpha-linolenic acid can be detected after the primary treatment of the pulean preparation without the purification of alpha-linolenic acid; and as the quantitative measurement is performed on the alpha-linolenic acid through gas chromatography, the test accuracy is high, and the reliability is good. The quality control method is very suitable for industrial application, and can be used for controlling the quality of a drug; and the inner quality of the product can be effectively controlled through the monitoring the alpha-linolenic acid in the pulean preparation.

Description

Technical field [0001] The invention relates to a method for quality control of Pulean preparations, more specifically a method for determining the content of alpha-linolenic acid in Pulean preparations. Background technique [0002] Pulean preparation is a tablet made from dried pollen of Brassica campestis Linn., a cruciferous plant rapeseed, collected by worker bees such as Apis cerana Fabricius and other worker bees. [0003] Pollen is the small granular material in the microsporangium on the angiosperm stamen anther and the microspore leaf of the spore plant, and is the male germ cell of the plant. Pollen contains a variety of rich nutrients, has a variety of biological activities, and has unique effects in treating prostate diseases. At present, there are pollen products such as "Qianliekang" and "Shenitong" on the market, which show good curative effects. These drugs have been used in the clinic for a long time, and the preparations have been proved to be effective in impr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 吴键叶剑锋王如伟胡江宁姚建标
Owner 浙江现代中药与天然药物研究院有限公司
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