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UPLC characteristic spectrum construction method and application of endothelium corneum gigeriae galli, fried endothelium corneum gigeriae galli and vinegar endothelium corneum

A characteristic map and technology of vinegar gallinaceous gold, applied in the field of analysis and detection, to achieve the effect of ensuring clinical curative effect

Active Publication Date: 2022-07-22
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing detection methods are difficult to effectively compare and analyze the differences and changes in the characteristic maps of gallinaceae and its processed products, decoction pieces, standard decoctions, and formula granules. The transmission of the technological process of the finished formula granules, it is difficult to overall evaluate and control the technological process from gallinaceae and its processed product decoction pieces to the finished formula granules

Method used

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  • UPLC characteristic spectrum construction method and application of endothelium corneum gigeriae galli, fried endothelium corneum gigeriae galli and vinegar endothelium corneum
  • UPLC characteristic spectrum construction method and application of endothelium corneum gigeriae galli, fried endothelium corneum gigeriae galli and vinegar endothelium corneum
  • UPLC characteristic spectrum construction method and application of endothelium corneum gigeriae galli, fried endothelium corneum gigeriae galli and vinegar endothelium corneum

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0089] The preparation of the test solution is to take the medicinal material of chicken Neijin, the decoction pieces of the chicken, the fried pieces of the chicken, and the decoction pieces of the chicken with vinegar, accurately weigh them, put them in a stoppered conical flask, add methanol, and reflux for 30 minutes. Allow to cool, supplement the lost weight with methanol, shake well, filter, and take the subsequent filtrate.

[0090] Wherein, the ratio of the mass g of the raw materials of the chicken Neijin medicinal material, the chicken Neijin decoction pieces, the fried chicken Neijin decoction pieces, and the vinegared chicken Neijin decoction pieces to the volume mL of the solvent is preferably 1:25.

[0091] Take chicken Neijin standard decoction, stir-fried chicken inner gold standard decoction, vinegar chicken inner gold standard decoction, chicken inner gold formula granules, fried chicken inner gold formula granules, vinegar chicken inner gold formula granules ...

Embodiment 1

[0155] Example 1 Screening of chromatographic conditions

[0156] 1.1 Chromatographic conditions

[0157] Use octadecylsilane-bonded silica gel as filler (column length is 150mm, inner diameter is 2.1mm, particle size is 1.6μm); methanol is used as mobile phase A, and 0.02% acetic acid is used as mobile phase B. Gradient elution was specified, and the detection wavelength was 260 nm; the flow rate was 0.3 ml / min, and the column temperature was 40 °C. The number of theoretical plates should not be less than 5000 according to the genistein peak. The mobile phase gradients are shown in Table 1.

[0158] Table 1

[0159]

[0160] 1.2.0 Mobile Phase Selection

[0161] On the basis of the experimental conditions proposed in 1.1 above, the separation effects of three different mobile phases were investigated: methanol-0.02% acetic acid, methanol-0.02% phosphoric acid, methanol-0.02% formic acid, see figure 1 , figure 1 Select Results Plot for Mobile Phase.

[0162] 1.2.1 Wa...

Embodiment 2

[0202] Example 2 Methodological investigation

[0203] 2.5.1 Chromatographic peak identification

[0204] Preparation of the test solution: According to the experimental conditions proposed above, prepare the chicken Neijin standard decoction for the test solution.

[0205] Preparation of control medicinal material solution: take about 1g of this product, accurately weigh it, add 25ml of water, weigh it, decoct for 30min, let it cool, make up the lost weight with water, shake well, filter, evaporate the filtrate to dryness, add 10ml of methanol , Dense plug, ultrasonic treatment (power 600W, frequency 40kHz) for 30 minutes, let cool, make up the lost weight with methanol, shake well, filter, and take the continuous filtrate.

[0206] Preparation of reference substance solution: Take appropriate amount of genistein, daidzein, genistein, and daidzin reference substance, accurately weigh, and add methanol to make a solution containing 8 μg per 1 ml.

[0207] Preparation of nega...

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Abstract

The invention provides a UPLC characteristic chromatogram construction method of endothelium corneum gigeriae galli decoction pieces, fried endothelium corneum gigeriae galli decoction pieces, vinegar endothelium corneum gigeriae galli decoction pieces, standard decoction of the endothelium corneum gigeriae galli decoction pieces and formula granules of the endothelium corneum gigeriae galli decoction Determining the liquid to be detected by adopting a high performance liquid chromatography to obtain UPLC characteristic chromatograms of the endothelium corneum gigeriae galli decoction pieces, the fried endothelium corneum gigeriae galli decoction pieces, the vinegar endothelium corneum gigeriae Chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 column; a mobile phase A is a methanol solution, a mobile phase B is a 0.02% acetic acid aqueous solution, and gradient elution is carried out. According to the method, the UPLC characteristic chromatogram for distinguishing the endothelium corneum gigeriae galli decoction pieces, the fried endothelium corneum gigeriae galli decoction pieces, the vinegar endothelium corneum gigeriae galli decoction pieces, the standard decoction and the formula granules of the endothelium corneum gigeriae galli decoction pieces, the fried endothelium corneum gigeriae galli decoction pieces and the vinegar endothelium corneum gigeriae galli decoction pieces is established,

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a method for constructing and application of UPLC characteristic maps of Chicken Neijin, fried Chicken Neijin, vinegared Chicken Neijin decoction pieces and their standard soups and granules. Background technique [0002] Chicken Neijin is the inner wall of the dry sandbag of Gallus gallus domesticus Brisson, a pheasant domesticated chicken. It is sweet and flat in nature and belongs to the spleen, stomach, small intestine and bladder meridians. It has the functions of invigorating the stomach and digesting food, astringent essence and relieving phlegm, and drenching fossils. For food accumulation, vomiting and diarrhea, infantile malnutrition, enuresis, nocturnal emission, astringent pain due to stone stranguria, gallbladder distention and hypochondrium pain. Chicken Neijin is clinically used raw, stir-fried, and vinegar-boiled. After processing, its efficacy an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N2030/065Y02A50/30
Inventor 周厚成周靖惟张晨胡昌江黄美华陈蓉胡庆张林黄宇梅国荣孙纪元邱亚磊
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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