Bacillus amyloliquefaciens and application thereof

A technology of amylolytic spores and bacilli, which is applied in the direction of bacteria, biological water/sewage treatment, biochemical equipment and methods, etc., to achieve high algae-dissolving effect

Inactive Publication Date: 2011-08-17
PEKING UNIV
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

At present, domestic research on it is limited to the optimization of fermentation conditions, the degradation of the herbicide butachlor (Tan Wenjie et al.; 2005), and the inhibition of plant pathogenic fungi and viruses (Shen Lili et al.; 2010) in biological control (Wang Yingying et al.; 2007 ), the research in the field of algae control is still blank

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  • Bacillus amyloliquefaciens and application thereof

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Embodiment Construction

[0014] The present invention will be further described below through a specific embodiment.

[0015] (1) Separation of Bacillus amyloliquefaciens CGMCC NO: 4516:

[0016] A water sample collector was used to collect water samples 1m below the surface of the eutrophic lake in Dianchi Lake, and the collected surface water samples were filtered through filter paper to eliminate the influence of protozoa and remove impurities in the water. 20 mL of filtered water samples were aseptically injected into 100 mL of Anabaena flos-aquae (Anabaena flos-aquae) pre-cultivated for 5 to 7 days and purchased from the Algae Bank of the Institute of Hydrobiology, Chinese Academy of Sciences (FACHB). )), the control group was injected with 20mL filtered autoclaved water samples, the algae were inoculated in a 150mL Erlenmeyer flask, sealed with a parafilm, and placed in a photosynthetic plant cultivation room for cultivation. The photosynthetic cultivation cycle was 14h:10h, The temperature is ...

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Abstract

The invention discloses a bacillus amyloliquefaciens and an application thereof. The bacillus amyloliquefaciens with the China general microbiological culture collection number 4516 (China general microbiological culture collection (CGMCC) NO: 4516) has an obvious inhibiting effect on the growth of algae, especially has a high-efficiency inhibiting effect on the growth of anabaena flos-aquae, and can be applied to algal bloom control or algal removal of water environments such as lakes, urban landscape water and the like which are in eutrophication state.

Description

technical field [0001] The invention relates to a bacterium, in particular to an algae-dissolving bacterium with high-efficiency algae-inhibiting effect. Background technique [0002] In the case of increasingly serious water eutrophication, economical, convenient and environmentally friendly biological methods ( et al., 1998; Carpenter et al., 1992) has become a research hotspot in the field of eutrophic water restoration. The research on algae control by algae-dissolving bacteria has been carried out gradually since the 1960s, and has been paid more and more attention. Domestic and foreign researchers have studied marine red tides (Imai et al.; 2001), eutrophic lakes (Rashidan et al.; 2001), reservoirs (S.W.Jung et al.; 2008), farmland drainage channels (Jeffrey; 1981), sewage (James et al.; 1969) , lake sediment (Choi Hee-jin et al.; 2005) and other environments isolated algicidal bacteria with anti-algae effect. [0003] Bacillus amyloliquefaciens is a non-pathogenic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07
Inventor 吴为中李超吴伟龙杨璐华安成才
Owner PEKING UNIV
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